Topoisomerase II alpha (TOP2A) Human siRNA Oligo Duplex (Locus ID 7153)
CAT#: SR322074
TOP2A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
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SR322074 is the updated version of SR304898.
CNY 4,090.00
货期*
4周
规格
Cited in 2 publications. |
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经常一起买 (3)
TOP2A mouse monoclonal antibody, clone OTI2A9 (formerly 2A9)
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Purity | HPLC purified |
Quality Control | Tested by ESI-MS |
Sequences | Available with shipment |
Stability | One year from date of shipment when stored at -20°C. |
# of transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Note | Single siRNA duplex (10nmol) can be ordered. |
Reference Data | |
RefSeq | NM_001067 |
Synonyms | TOP2; TOP2alpha; TOPIIA; TP2A |
Components | TOP2A (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 7153)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml |
Summary | This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010] |
Performance Guranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
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TOP2A Promotes Cell Migration, Invasion and Epithelial–Mesenchymal Transition in Cervical Cancer via Activating the PI3K/AKT Signaling
,Wang, B;Shen, Y;Zou, Y;Qi, Z;Huang, G;,
Cancer Management and Research
,PubMed ID 32547216
[TOP2A]
|
Cellular DNA topoisomerases are required for the synthesis of hepatitis B virus covalently closed circular DNA
,Sheraz, M;Cheng, J;Tang, L;Chang, J;Guo, JT;,
J. Virol.
,PubMed ID 30867306
[TOP2A]
|
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