WRN Human siRNA Oligo Duplex (Locus ID 7486)
CAT#: SR322215
WRN (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each
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SR322215 is the updated version of SR305118.
CNY 4,090.00
货期*
4周
规格
Cited in 2 publications. |
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经常一起买 (3)
Specifications
Product Data | |
Purity | HPLC purified |
Quality Control | Tested by ESI-MS |
Sequences | Available with shipment |
Stability | One year from date of shipment when stored at -20°C. |
# of transfections | Approximately 330 transfections/2nmol in 24-well plate under optimized conditions (final conc. 10 nM). |
Note | Single siRNA duplex (10nmol) can be ordered. |
Reference Data | |
RefSeq | NM_000553 |
Synonyms | RECQ3; RECQL2; RECQL3 |
Components | WRN (Human) - 3 unique 27mer siRNA duplexes - 2 nmol each (Locus ID 7486)Included - SR30004, Trilencer-27 Universal Scrambled Negative Control siRNA Duplex - 2 nmolIncluded - SR30005, RNAse free siRNA Duplex Resuspension Buffer - 2 ml |
Summary | This gene encodes a member of the RecQ subfamily of DNA helicase proteins. The encoded nuclear protein is important in the maintenance of genome stability and plays a role in DNA repair, replication, transcription and telomere maintenance. This protein contains a N-terminal 3' to 5' exonuclease domain, an ATP-dependent helicase domain and RQC (RecQ helicase conserved region) domain in its central region, and a C-terminal HRDC (helicase RNase D C-terminal) domain and nuclear localization signal. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by accelerated aging and an elevated risk for certain cancers. [provided by RefSeq, Aug 2017] |
Performance Guranteed | OriGene guarantees that at least two of the three Dicer-Substrate duplexes in the kit will provide at least 70% or more knockdown of the target mRNA when used at 10 nM concentration by quantitative RT-PCR when the TYE-563 fluorescent transfection control duplex (cat# SR30002) indicates that >90% of the cells have been transfected and the HPRT positive control (cat# SR30003) provides 90% knockdown efficiency. For non-conforming siRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the siRNA kit. To arrange for a free replacement with newly designed duplexes, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled siRNA control (quantitative RT-PCR data required). |
Citations (2)
The use of this RNAi has been cited in the following citations: |
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PARP1 allows proper telomere replication through TRF1 poly (ADP-ribosyl)ation and helicase recruitment
,null,
Communications Biology
,PubMed ID 36864251
[WRN]
|
NAD+ augmentation restores mitophagy and limits accelerated aging in Werner syndrome
,Fang, EF;Hou, Y;Lautrup, S;Jensen, MB;Yang, B;SenGupta, T;Caponio, D;Khezri, R;Demarest, TG;Aman, Y;Figueroa, D;Morevati, M;Lee, HJ;Kato, H;Kassahun, H;Lee, JH;Filippelli, D;Okur, MN;Mangerich, A;Croteau, DL;Maezawa, Y;Lyssiotis, CA;Tao, J;Yokote, K;Rusten, TE;Mattson, MP;Jasper, H;Nilsen, H;Bohr, VA;,
Nat Commun
,PubMed ID 31754102
[WRN]
|
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