pAAVS1-EF1a-puro-DNR CRISPR Vector

CAT＃: GE100046

AAVS1 donor vector with AAVS1 homologous arms cloned (Puro selection), EF1a promoter, ready for exogenous gene cloning (10 ug)

Custom Cloning into Donor Vector Request

Datasheet
SDS
Vector Sequence

CNY 7,510.00

货期*
现货
规格
    • 10 ug
Cited in 1 publication.

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TP790148
Purified recombinant protein of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E.coli, sterile-filtered, suitable for cell culture
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TP790151
Purified recombinant protein of mutant(D10A) of S. pyogenes Cas9 endonuclease (Cas9) containing Simian virus 40 (SV40) T antigen nuclear localization sequence (NLS) on the N- and C- termini of the protein., with N-terminal HIS tag, expressed in E. coli
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CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
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Specifications

Product Data
Function AAVS1 donor vector
Features
  • Donor vector for targeted transgene insertion into human AAVS1 site
  • Contains left and right AAVS1 homologous sequences (550 bp each side)
  • Multiple cloning sites for transgene cloning, compatible for shuttling from the TrueORF clones
  • The expression of the target gene will be under EF1a promoter, a constitutive promoter as an alternative of CMV promoter. the latter could be silenced in certain cellular environment
  • Puromycin mammalian selection under PGK promoter
  • Targeted insertion when cotransfected with pCas-Guide-AAVS1 (SKU GE100023)
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (1)

The use of this Vectors has been cited in the following citations:
Transplantation of hMSCs Genome Edited with LEF1 Improves Cardio-Protective Effects in Myocardial Infarction ,Cho, H;Lee, K;Shen, Y;Shin, T;Ryu, P;Choi, M;Kang, K;Cho, J;, Molecular Therapy - Nucleic Acids ,PubMed ID 32069701 [pCas-Guide-AAVS1]

Documents

Product Manuals
FAQs
SDS
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