Proteins

Proteins

Recombinant Proteins

Human

KRT20

E. coli

10 mM Tris-HCl

2 mM dithiothreitol

Protein standard in 1D and 2D SDS gelelectrophoresis.
Immunoassays.
Immunization.
Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml

both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl

e.g. Dulbecco's PBS).

pH 7.4). For immunization purposes

the solution can be further dialyzed against PBS (phosphate buffered saline

2