Cytokeratin 20 Human Protein

Specifications

Product Data
Species	Human
Expression Host	E. coli
Predicted MW	46 kDa
Concentration	lot specific
Purity	>95% determined by SDS gelelectrophoresis
Buffer	Presentation State: Purified State: Lyophilised purified protein Buffer System: 30 mM Tris/HCI pH 8, 9.5 M urea, 2 mM DTT, 2 mM EDTA, 10 mM methylammonium chloride
Preparation	Lyophilised purified protein
Applications	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).
Protein Description	Recombinant human keratin K20 (formerly also designated cytokeratin 20).
Note	Isoelectric Point pI 5.66
Storage	Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody at -20°C. Avoid repeated freezing and thawing.
Stability	Shelf life: one year from despatch.
Reference Data
RefSeq	NP_061883
Locus ID	54474
UniProt ID	P35900
Cytogenetics	17q21.2
Synonyms	Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT
Summary	The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Protein Families	Protein standard in 1D and 2D SDS gelelectrophoresis. Immunoassays. Immunization. Protocol: Reconstitution to filaments is performed by mixing equimolar amounts of keratins of type I and type II at concentrations of approx. 0.5 mg/ml, both dissolved in 9.5 M urea buffer (see above). Protofilaments and filament complexes are obtained by dialyzing the resulting polypeptide solution stepwise to a concentration of 4 M urea and then to low salt condition (50 mM NaCl, 2 mM dithiothreitol, 10 mM Tris-HCl, pH 7.4). For immunization purposes, the solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbecco's PBS).

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