Rituximab Rat Monoclonal Antibody [Clone ID: MB2 A4]

Specifications

Product Data
Clone Name	MB2 A4
Applications	ELISA, FC
Recommend Dilution	ELISA: 5 µg/ml as coating antibody. Flow Cytometry: Use 10 µl of 50 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	F(ab)2 fragment of Rituximab. Spleen cells from immunised rats were fused with cells of the NS-1 mouse myeloma cell line
Specificity	This antibody specifically recognises the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. It does not recognise other CD20 antibodies. This antibody has been used in ELISA assays to monitor the levels of Rituximab in patient serum following therapy. It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3).
Formulation	TRIS buffered saline pH 8.0 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Background	Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.
Synonyms	Rituxan, MabThera
Reference Data

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