Rituximab Rat Monoclonal Antibody [Clone ID: MB2 A4]

Specifications

Product Data
Clone Name	MB2 A4
Applications	FC
Recommend Dilution	Flow Cytometry: Use 10 µl of neat antibody to label 106 cells in 100 µl.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	F(ab)2 fragment of Rituximab
Specificity	This antibody specifically recognizes the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody. This antibody does not recognise other CD20 antibodies. It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3).
Formulation	PBS, pH 7.4 Label: FITC State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: 1% BSA Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	FITC
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light.  Avoid repeated freezing and thawing.
Background	Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.
Synonyms	Rituxan, MabThera
Reference Data

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