Rituximab Rat Monoclonal Antibody [Clone ID: MB2 A4]

Specifications

Product Data
Clone Name	MB2 A4
Applications	ELISA
Recommend Dilution	ELISA: 1/20000-1/20000.
Reactivity	Human
Host	Rat
Clonality	Monoclonal
Immunogen	F(ab)2 fragment of Rituximab
Specificity	This antibody specifically recognises the idiotypic determinants expressed by the Rituximab humanised monoclonal antibody.  This antibody does not recognise other CD20 antibodies. It has been used to detect Rituximab bound to the surface of the Raji B cell line, however detection of Rituximab bound 'in vivo' to B-CLL cells has not been demonstrated. It is possible that complement deposition on Rituximab opsonised cells inhibits binding of the anti-Rituximab antibody to cell bound Rituximab (3). Clone MB2A4 has been used in ELISA assays to monitor the levels of rituximab in patient serum following therapy (4,5).
Formulation	PBS Label: HRP State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Stabilizer: HRP stabilizer (containg no Azide, no Mercury, no BSA or other Bovine material, but weak concentrations of Rabbit antibodies). Preservative: 0.01% Thiomersal
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	HRP
Storage Condition	Store undiluted at 2-8°C. DO NOT FREEZE!
Background	Rituximab is a therapeutic reagent directed against the human CD20 cell surface antigen.
Synonyms	Rituxan, MabThera
Reference Data

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