TNF alpha (TNF) Mouse Monoclonal Antibody [Clone ID: Mab11]

Specifications

Product Data
Clone Name	Mab11
Applications	FC, FN, IF, IHC
Recommend Dilution	Flow cytometry: Intracellular staining Immunohistochemistry (frozen sections): paraformaldehyde-fixed, saponin-treated frozen tissue sections. Immunocytochemistry. Functional application: Neutralization.
Reactivity	Human, Porcine, Primate
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant human TNF-alpha
Specificity	The mouse monoclonal antibody MAb11 recognizes 17-26 kDa cytokine TNF-alpha (tumor necrosis factor alpha).
Formulation	Phosphate buffered saline (PBS), pH 7.4 State: Aff - Purified State: Liquid Ig fraction Preservative: 15 mM sodium azide
Concentration	lot specific
Purification	Protein-A affinity chromatography (> 95% pure by SDS-PAGE)
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor
Database Link	Entrez Gene 7124 Human UniProt ID P01375
Background	TNF-alpha is a cytokine produced by monocytes, macrophages, neutrophils, NK cells, CD4+ T cells and many transformed cells. It can be expressed as a 17 kDa free molecule, or as a 26 kDa membrane protein. TNF-alpha easily forms stable trimers, but also other multimeric complexes. In the immune system, it is an important regulator, which has cytolytic and cytostatic activity against a range of tumor cells, increases fibroblast proliferation and supports neutrophil chemotaxis and phagocytosis.
Synonyms	TNF, TNF-a, TNFA, TNFSF2, Cachectin
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.