CCN3 (NOV) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Indirect ELISA: To detect hNOV by (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antibody in conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Sandwich ELISA: To detect hNOV (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human NOV (AP01154BT-S or AP01154BT-N) as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Western blot: To detect hNOV this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Formalin-Fixed, Paraffin Sections: 0.25-0.50 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Positive Control: Human breast invasive ductal carcinoma.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) recombinant Human NOV
Specificity	This antibody detects NOV.
Formulation	PBS, pH 7.2 State: Aff - Purified State: Lyophilized (sterile filtered) Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Immunoaffinity Chromatography
Conjugation	Unconjugated
Storage Condition	Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	nephroblastoma overexpressed
Database Link	Entrez Gene 4856 Human UniProt ID P48745
Background	CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation.
Synonyms	CCN3, IGFBP9, NOVH, NovH
Note	Centrifuge vial prior to opening.
Reference Data

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