CCN3 (NOV) Rabbit Polyclonal Antibody
CAT#: AP01154PU-S
CCN3 (NOV) rabbit polyclonal antibody, Aff - Purified
Size: 100 ug
Conjugation: Biotin
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CNY 3,910.00
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Specifications
Product Data | |
Applications | ELISA, IHC, WB |
Recommend Dilution | Indirect ELISA: To detect hNOV by (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antibody in conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Sandwich ELISA: To detect hNOV (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. In conjunction with Biotinylated Anti-Human NOV (AP01154BT-S or AP01154BT-N) as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant hNOV. Western blot: To detect hNOV this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNOV is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Formalin-Fixed, Paraffin Sections: 0.25-0.50 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer is recommended. Positive Control: Human breast invasive ductal carcinoma. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure (> 98%) recombinant Human NOV |
Specificity | This antibody detects NOV. |
Formulation | PBS, pH 7.2 State: Aff - Purified State: Lyophilized (sterile filtered) Ig fraction |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Immunoaffinity Chromatography |
Conjugation | Unconjugated |
Storage Condition | Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing. |
Gene Name | nephroblastoma overexpressed |
Database Link | |
Background | CCN3 is a cysteine-rich protein that is overexpressed in avian nephroblastomas. It is a member of the CCN family of proteins that includes CTGF. These proteins are encoded by a group of genes known as immediate-early genes, so named because they are expressed after induction by growth factors or certain oncogenes. The proteins share several common structural motifs: a consensus sequence present in IGF (insulin-like growth factor)-binding proteins (the IGFBP motif), an oligomeric complex-forming domain first identified in von Willebrand factor, a binding domain to soluble and matrix molecules and a dimerization (CT) domain. All CCN family members are thought to be involved in the control of cell proliferation. |
Synonyms | CCN3, IGFBP9, NOVH, NovH |
Note | Centrifuge vial prior to opening. |
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