Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody [Clone ID: KS20.10]

Specifications

Product Data
Clone Name	KS20.10
Applications	FC, IF, IHC
Recommend Dilution	FACS. Cytological material. Immunohistochemistry on Frozen sections. Immunohistochemistry on Paraffin sections (Only after Microwave treatment). Recommended Dilutions: 1/10-1/20 in PBS, PH 7.4 for Immunohistochemistry. Incubation Time: 1h at RT or over night at 2-8°C.
Reactivity	Human, Porcine, Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Electrophoretically purified Keratin K20 from Human intestinal mucosa.
Specificity	This Monoclonal clone IT-Ks20.10 antibody represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide Recognized: Protein IT (keratin K20; Mr 46 000; also designated Cytokeratin 20). Tested Reactivities on Cultured Cell Lines: HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
Formulation	Label: FITC State: Liquid purified Ig fraction with 0.09% Sodium Azide and 0.5% BSA
Concentration	lot specific
Purification	Affinity Chromatography
Conjugation	FITC
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing.
Gene Name	keratin 20
Database Link	Entrez Gene 54474 Human UniProt ID P35900
Background	Cytokeratin 20 is a 46 kDa intermediate filament protein whose expression is restricted primarily to gastric and intestinal epithelium, urothelium, and Merkel cells. This antibody is highly specific to cytokeratin 20 and shows no cross-reactivity with other IFPs. Cytokeratin 20 is a unique type I keratin that is expressed in adenocarcinomas of the colon stomach, pancreas and bile system. It is also expressed in mucinous ovarian tumors, transitional cell carcinomas of the urinary tract, and Merkel cell carcinomas.
Synonyms	Cytokeratin 20, Keratin-20, KRT20, CK20, K20, Protein IT
Reference Data

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