Itgam Mouse Monoclonal Antibody [Clone ID: WT.3]

Specifications

Product Data
Clone Name	WT.3
Applications	FC, FN, IP, WB
Recommend Dilution	Flow cytometry: 1/10 - 1/50; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl. Immunoprecipitation. Immunohistochemistry on frozen sections.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	FTL-43. Lymph node cells from immunised BALB/c mice were fused with cells from the PAI mouse myeloma cell line.
Specificity	This antibody reacts with the beta 2 integrin, which is designated CD18. The beta 2 integrin may be associated with the integrin alphaL, alphaX or alphaM subunits. Clone WT.3 has been shown to inhibit homotypic aggregation of PHA blasts. It also blocks binding of rat lymphocytes to purified rat ICAM-1. SM264A is recommended for this purpose.
Formulation	PBS, pH7.2 containing 0.09% Sodium Azide State: Purified State: Liquid purified IgG
Concentration	lot specific
Purification	Affinity chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha M
Database Link	Entrez Gene 25021 Rat UniProt ID Q9JI30
Background	CD18 is expressed by all leucocytes.
Synonyms	Integrin beta-2, MFI7
Reference Data

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