FOXP3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	FC, ICC/IF, IHC, Immunoblotting, IP, WB
Recommend Dilution	Single Cell Western: 100 ug/mL, Western Blot: 1:2000, Flow Cytometry: 1:8000, Immunocytochemistry/ Immunofluorescence: 1:500, Immunoprecipitation, Immunohistochemistry-Paraffin: 1:400, Immunoblotting, Immunohistochemistry: 1:400, Dual RNAscope ISH-IHC
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	A peptide corresponding to amino acids 43-100 of mouse FOXP3. [Swiss-Prot# Q99JB6]
Formulation	Tris-citrate/phosphate, pH 7, 0.1% Sodium azide. Store at 4C. Do not freeze.
Concentration	lot specific
Purification	Protein G purified
Conjugation	Unconjugated
Storage Condition	Store at -20°C as received.
Gene Name	forkhead box P3
Database Link	NP_054728 Entrez Gene 20371 MouseEntrez Gene 50943 Human UniProt ID Q9BZS1
Background	FOXP3 (a 381 amino acid protein) is a member of the forkhead/winged-helix family of transcriptional regulators and is highly conserved across mammals. FOXP3 is essential for normal immune homeostasis. FOXP3 is stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene and a more specific marker of regulatory T cells than other T cells.
Synonyms	AIID; DIETER; IPEX; JM2; PIDX; XPID
Note	This FOXP3 antibody can be used for Immunoprecipitation, Immunohistochemistry paraffin embeded sections and Western blot analysis, where a band is seen ~48 kDa in FOXP3 transfected and CD25/CD4 positive regulatory T cell lysates. In IP transfected FOXP3-HA tagged proteins were used. In IHC-P, staining was observed in the nuclei of mouse spleen cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families	Transcription Factors

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