Human IgG4 (pFC specific) Mouse Monoclonal Antibody [Clone ID: NI 315 (HP 6206)]

Specifications

Product Data
Product Name	Human IgG4 (pFC specific) Mouse Monoclonal Antibody [Clone ID: NI 315 (HP 6206)]
Clone Name	NI 315 (HP 6206)
Applications	ELISA, IF, WB
Recommend Dilution	To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. General Recommended Dilutions: Histochemical Use: 1/50-1/250.  ELISA: from 1/100 upwards.  Western blot: from 1/500 upwards.
Reactivity	Human
Host	Mouse
Immunogen	Highly purified monoclonal IgG4 isolated from pooled human serum.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized purified IgG fraction Molar radio: Biotin/IgG ~4.8
Concentration	0.4 mg/ml
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Note	Conjugation Procedure: Conjugation is carried out using a proprietary technique for the binding of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.