Human IgG4 (pFC specific) Mouse Monoclonal Antibody [Clone ID: NI 315 (HP 6206)]

Specifications

Product Data
Product Name	Human IgG4 (pFC specific) Mouse Monoclonal Antibody [Clone ID: NI 315 (HP 6206)]
Clone Name	NI 315 (HP 6206)
Applications	ELISA, IF, IP, WB
Recommend Dilution	To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Direct Immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards.
Reactivity	Human
Host	Mouse
Immunogen	Highly purified monoclonal IgG4 isolated from pooled Human serum.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: HRP State: Lyophilized purified IgG fraction Label: Horseradish Peroxidase Molar radio: Peroxidase/IgG ~1.7
Concentration	0.4 mg/ml
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Note	Conjugation Procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added.

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