Human IgG4 (pFC specific) Mouse Monoclonal Antibody [Clone ID: NI 315 (HP 6206)]

Specifications

Product Data
Product Name	Human IgG4 (pFC specific) Mouse Monoclonal Antibody [Clone ID: NI 315 (HP 6206)]
Clone Name	NI 315 (HP 6206)
Applications	ELISA, IF, IP, WB
Recommend Dilution	The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. General Recommended Dilutions: Histochemical Use: 1/10-1/50 ELISA: from 1/200 upwards Western blot: from 1/400 upwards
Reactivity	Human
Host	Mouse
Immunogen	Highly purified monoclonal IgG4 isolated from Human serum.
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: TRITC State: Lyophilized purified IgG fraction Label: Tetramethylrhodamine Isothiocyanate Absorption emission: 554 nm / 573 nm Molar radio: Fluorochrome/IgG ~2.7
Concentration	0.4 mg/ml
Conjugation	TRITC
Storage Condition	Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing.

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