Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]

Specifications

Product Data
Product Name	Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
Clone Name	NI 179
Applications	ELISA, IF, IP, WB
Recommend Dilution	To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgM, and Immunoblotting. As a second step an Avidin or Streptavidin conjugate have to be used. General Recommended Dilutions: Histochemical Use: 1/100-1/500 ELISA: from 1/1,000 upwards Western blot: from 1/2,000 upwards
Reactivity	Human
Host	Mouse
Immunogen	Highly purified monoclonal IgM isolated from pooled Human serum
Formulation	PBS, pH 7.2 without preservatives and foreign proteins Label: Biotin State: Lyophilized purified IgG fraction Molar radio: Biotin/IgG: ~7.1
Concentration	0.4 mg/ml
Purification	Immunoaffinity Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing.
Note	Conjugation Procedure: Conjugation is carried out using a proprietary technique for the binding of biotin, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.