Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]

Specifications

Product Data
Product Name	Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
Clone Name	NI 179
Applications	ELISA, IF, IHC, IP, WB
Recommend Dilution	ELISA. Dot blot. Immunoblotting. Immunocytochemistry. Immunohistochemistry on Paraffin Sections. To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/250 upwards. Western blot: from 1/500 upwards.
Reactivity	Human
Host	Mouse
Immunogen	Highly purified monoclonal IgM isolated from pooled Human serum
Formulation	PBS, pH 7.2 without foreign proteins Label: HRP State: Lyophilized purified IgG fraction Preservative: None as it may interfere with the antibody activity Label: Horseradish Peroxidase enriched for isoenzyme C (RZ=3.2) Molar radio: Peroxidase/IgG: ~1.7
Concentration	0.4 mg/ml
Purification	Immunoaffinity Chromatography
Conjugation	HRP
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Note	Conjugation procedure: Conjugation is carried out using a proprietary modification of the periodate method, followed by several purification steps. After each step activity and specificity are tested in a variety of techniques. No foreign protein has been added. The conjugate is lyophilized to assure stability and long shelf life

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.