Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179]
CAT#: AM20259FC-N
Human IgM mouse monoclonal antibody, clone NI 179, FITC
Conjugation: Unconjugated Biotin HRP TRITC
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CNY 8,794.00
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Specifications
Product Data | |
Product Name | Human IgM Mouse Monoclonal Antibody [Clone ID: NI 179] |
Clone Name | NI 179 |
Applications | ELISA, IF, IP, WB |
Recommend Dilution | To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect Immunofluorescence staining of cytoplasmic IgM and cell membrane IgM. General Recommended Dilutions: Histochemical Use: 1/10-1/50 |
Reactivity | Human |
Host | Mouse |
Immunogen | Highly purified monoclonal IgM isolated from pooled Human serum |
Formulation | PBS, pH 7.2 without preservatives and foreign proteins Label: FITC State: Lyophilized purified IgG fraction Label: Fluorescein Isothiocyanate Conjugation Procedure: A proprietary technique for the binding to is used, followed by several purification steps to remove free reactants and protein aggregates. After each step activity and specificity are tested in a variety of techniques. The conjugate is lyophilized to assure stability and long shelf life Absorption emission: 492 nm / 515 nm Molar radio: Fluorochrome/IgG: ~1.4 |
Concentration | 0.4 mg/ml |
Purification | Immunoaffinity Chromatography |
Conjugation | FITC |
Storage Condition | Store the antibody lyophilized at 2-8°C for one month or (in aliquots) at -20°C for longer. Diluted ascites should be stored at 2-8°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. Avoid repeated thawing and freezing. |
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