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Tdp2 (NM_019551) Mouse Untagged Clone
CAT＃: MC210026
Tdp2 (untagged) - Mouse tyrosyl-DNA phosphodiesterase 2 (Tdp2), (10ug)
CNY 3,990.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | D13Ertd656e; Ttrap |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC210026 representing NM_019551
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCTGGCAGCAGTTCCGATGCGGCGGAGCCCGCAGGGCCGGCAGGGCGGGCGGCGTCGGCGCCCG AAGCAGCACAGGCGGAGGAGGACCGGGTGAAGAGGCGGCGGCTTCAGTGCCTGGGCTTTGCGTTGGTGGG GGGATGCGACCCCACGATGGTCCCCAGCGTCCTGCGGGAGAACGACTGGCAGACGCAGAAAGCCCTGAGC GCCTACTTCGAGCTGCCAGAGAACGACCAAGGGTGGCCGCGCCAGCCTCCCACGTCCTTCAAGTCCGAGG CCTATGTTGATCTAACCAACGAGGATGCAAATGATACAACCATTTTAGAAGCCAGTCCATCTGGAACTCC TCTAGAAGATAGCAGCACTATTTCTTTCATTACCTGGAATATTGATGGATTAGATGGATGCAATCTGCCC GAGAGGGCTCGAGGGGTGTGTTCCTGCCTAGCTTTGTATAGTCCAGATGTGGTATTTCTACAGGAAGTTA TCCCCCCATACTGTGCCTACCTAAAGAAGAGAGCAGCCAGTTACACAATTATTACAGGTAATGAAGAAGG ATATTTCACAGCTATACTATTGAAGAAAGGAAGAGTGAAATTTAAAAGTCAGGAGATTATTCCTTTTCCA AATACCAAAATGATGAGAAACCTGCTATGCGTAAATGTGAGTTTGGGTGGAAATGAATTTTGCCTTATGA CATCCCATTTGGAGAGCACCAGAGAACATTCTGCGGAACGAATAAGACAATTAAAAACTGTTCTTGGAAA AATGCAAGAGGCTCCAGATTCAACCACGGTTATATTTGCAGGAGATACAAATTTAAGAGATCAAGAAGTT ATCAAATGTGGTGGTTTACCTGACAACGTTTTTGATGCCTGGGAATTTTTAGGCAAACCTAAACATTGCC AGTATACATGGGATACGAAAGCAAATAACAACCTCAGGATCCCTGCTGCTTATAAGCATCGTTTTGATCG AATATTTTTCAGAGCAGAAGAGGGGCACCTTATTCCTCAAAGTTTAGACCTTGTTGGGTTGGAAAAACTG GACTGTGGTAGATTTCCGAGTGATCACTGGGGGCTCTTGTGCACCTTGAATGTAGTATTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_019551 |
| Insert Size | 1113 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019551.2, NP_062424.1 |
| RefSeq Size | 1977 bp |
| RefSeq ORF | 1113 bp |
| Locus ID | 56196 |
| UniProt ID | Q9JJX7 |
| Gene Summary | DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation (PubMed:23104055, PubMed:24808172, PubMed:27099339, PubMed:27060144). Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2 (PubMed:22740648). Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. Acts as a regulator of ribosome biogenesis following stress (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG225243 | Tdp2 (tGFP-tagged) - Mouse tyrosyl-DNA phosphodiesterase 2 (Tdp2), (10ug) |
CNY 3,710.00 |
|
| MR225243 | Tdp2 (Myc-DDK-tagged) - Mouse tyrosyl-DNA phosphodiesterase 2 (Tdp2) |
CNY 3,656.00 |
|
| MR225243L3 | Lenti ORF clone of Tdp2 (Myc-DDK-tagged) - Mouse tyrosyl-DNA phosphodiesterase 2 (Tdp2) |
CNY 5,230.00 |
|
| MR225243L4 | Lenti ORF clone of Tdp2 (mGFP-tagged) - Mouse tyrosyl-DNA phosphodiesterase 2 (Tdp2) |
CNY 5,230.00 |
