Tdp2 (NM_019551) Mouse Tagged ORF Clone

CAT#: MG225243

  • TrueORF®

Tdp2 (tGFP-tagged) - Mouse tyrosyl-DNA phosphodiesterase 2 (Tdp2), (10ug)

ORF Plasmid: DDK tGFP



  "NM_019551" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,710.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms D13Ertd656e; Ttrap
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG225243 representing NM_019551
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTCTGGCAGCAGTTCCGATGCGGCGGAGCCCGCAGGGCCGGCAGGGCGGGCGGCGTCGGCGCCCG
AAGCAGCACAGGCGGAGGAGGACCGGGTGAAGAGGCGGCGGCTTCAGTGCCTGGGCTTTGCGTTGGTGGG
GGGATGCGACCCCACGATGGTCCCCAGCGTCCTGCGGGAGAACGACTGGCAGACGCAGAAAGCCCTGAGC
GCCTACTTCGAGCTGCCAGAGAACGACCAAGGGTGGCCGCGCCAGCCTCCCACGTCCTTCAAGTCCGAGG
CCTATGTTGATCTAACCAACGAGGATGCAAATGATACAACCATTTTAGAAGCCAGTCCATCTGGAACTCC
TCTAGAAGATAGCAGCACTATTTCTTTCATTACCTGGAATATTGATGGATTAGATGGATGCAATCTGCCC
GAGAGGGCTCGAGGGGTGTGTTCCTGCCTAGCTTTGTATAGTCCAGATGTGGTATTTCTACAGGAAGTTA
TCCCCCCATACTGTGCCTACCTAAAGAAGAGAGCAGCCAGTTACACAATTATTACAGGTAATGAAGAAGG
ATATTTCACAGCTATACTATTGAAGAAAGGAAGAGTGAAATTTAAAAGTCAGGAGATTATTCCTTTTCCA
AATACCAAAATGATGAGAAACCTGCTATGCGTAAATGTGAGTTTGGGTGGAAATGAATTTTGCCTTATGA
CATCCCATTTGGAGAGCACCAGAGAACATTCTGCGGAACGAATAAGACAATTAAAAACTGTTCTTGGAAA
AATGCAAGAGGCTCCAGATTCAACCACGGTTATATTTGCAGGAGATACAAATTTAAGAGATCAAGAAGTT
ATCAAATGTGGTGGTTTACCTGACAACGTTTTTGATGCCTGGGAATTTTTAGGCAAACCTAAACATTGCC
AGTATACATGGGATACGAAAGCAAATAACAACCTCAGGATCCCTGCTGCTTATAAGCATCGTTTTGATCG
AATATTTTTCAGAGCAGAAGAGGGGCACCTTATTCCTCAAAGTTTAGACCTTGTTGGGTTGGAAAAACTG
GACTGTGGTAGATTTCCGAGTGATCACTGGGGGCTCTTGTGCACCTTGAATGTAGTATTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG225243 representing NM_019551
Red=Cloning site Green=Tags(s)

MASGSSSDAAEPAGPAGRAASAPEAAQAEEDRVKRRRLQCLGFALVGGCDPTMVPSVLRENDWQTQKALS
AYFELPENDQGWPRQPPTSFKSEAYVDLTNEDANDTTILEASPSGTPLEDSSTISFITWNIDGLDGCNLP
ERARGVCSCLALYSPDVVFLQEVIPPYCAYLKKRAASYTIITGNEEGYFTAILLKKGRVKFKSQEIIPFP
NTKMMRNLLCVNVSLGGNEFCLMTSHLESTREHSAERIRQLKTVLGKMQEAPDSTTVIFAGDTNLRDQEV
IKCGGLPDNVFDAWEFLGKPKHCQYTWDTKANNNLRIPAAYKHRFDRIFFRAEEGHLIPQSLDLVGLEKL
DCGRFPSDHWGLLCTLNVVL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_019551
ORF Size 1110 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_019551.2, NP_062424.1
RefSeq Size 1977 bp
RefSeq ORF 1113 bp
Locus ID 56196
UniProt ID Q9JJX7
Gene Summary DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DNA double-strand breaks/DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation (PubMed:23104055, PubMed:24808172, PubMed:27099339, PubMed:27060144). Thereby, protects the transcription of many genes involved in neurological development and maintenance from the abortive activity of TOP2 (PubMed:22740648). Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DSBs due to DNA damage by radiation and free radicals. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'-tyrosyl-DNA phosphodiesterase in cells. Also acts as an adapter by participating in the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF-beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non-canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. Acts as a regulator of ribosome biogenesis following stress (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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