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Ern1 (NM_023913) Mouse Untagged Clone
CAT＃: MC222914
Ern1 (untagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), (10ug)
CNY 7,160.00
Product images
Specifications
| Product Data | |
| Type | Mouse Untagged Clone |
| Tag | Tag Free |
| Synonyms | 9030414B18Rik; AI225830; C85377; Ire1a; Ire1alpha; Ire1p |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MC222914 representing NM_023913
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGCCCGGTGGCTGTTGCTCCTGCTGGCGCTGCTGCTACCGCCGCCCGGCCCCGGGAGTTTTGGAA GAACCAGCACAGTTACACTGCCTGAGACCTTGTTGTTTGTCTCGACCCTGGATGGAAGCTTGCATGCTGT TAGCAAGAGGACGGGCTCCATCAAGTGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACACGTG GAAGAGCCGGCTTTCCTCCCAGATCCCAATGATGGCAGTCTGTACACACTTGGAGGCAAGAACAACGAAG GCCTGACGAAACTTCCCTTTACCATCCCAGAATTGGTTCAGGCCTCCCCATGCCGAAGTTCAGATGGAAT CCTCTACATGGGTAAAAAGCAAGATATTTGGTATGTTATCGACCTCCTGACTGGCGAGAAGCAGCAGACT TTGTCATCGGCCTTTGCTGATAGTCTCTGCCCATCAACTTCCCTTCTATATCTTGGACGGACAGAATACA CCATCACCATGTATGACACCAAGACCCGGGAGCTCCGCTGGAATGCCACCTATTTTGACTATGCAGCCTC ACTGCCGGAAGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGCGATGGACTGGTGGTAACT GTGGACAGTGAATCTGGGGATGTCCTGTGGATCCAAAACTATGCCTCTCCTGTGGTGGCCTTCTACGTCT GGCAGGGGGAGGTCCTGAGAAAGGTGGTGCACATCAACGTTGCTGTGGAGACTCTACGCTACTTGACCTT CATGTCTGGGGAAGTGGGGCGCATCACCAAGTGGAAGTATCCATTCCCCAAGGAGACAGAGGCCAAGAGC AAGCTAACGCCTACTCTGTATGTTGGGAAGTATTCCACCAGCCTCTATGCCTCTCCCTCAATGGTGCATG AGGGGGTTGCTGTCGTGCCTCGAGGCAGCACTCTTCCTTTGCTGGAAGGCCCCCAGACAGATGGCGTCAC CATTGGAGACAAAGGAGAGTGTGTGATCACTCCCAGCACAGACCTCAAGTTTGACCCTGGACTCAAAGGG AAGAGCAAGCTGAACTACTTGAGGAATTACTGGCTTCTCATAGGACACCATGAAACTCCTCTGTCTGCAT CCACCAAGATGCTGGAGAGATTTCCTAACAACCTGCCCAAACATCGAGAAAATGTGATTCCTGCTGATTC AGAAAAAAGGAGCTTTGAGGAAGTTATCAACATAGTTGGCCAGACTTCAGACAACACACCGACCACCGTA TCTCAGGATGTGGAGGAGAAGCTCGCTCGCGCCCCTGCCAAGCCTGAGGCCCCCGTGGACTCCATGCTCA AGGACATGGCTACCATTATCCTGAGCACCTTCCTGCTGGTTGGATGGGTGGCGTTCATCATCACTTACCC CCTGAGCGTGCATCAGCAGCGTCAGCTCCAGCACCAACAGTTCCAGAAGGAGCTGGAGAAGATTCAGCTC CTGCAGCAGCAGCAGCTGCCCTTCCACCCACACGGAGACCTTACCCAGGACCCTGAGTTCCTGGATTCAT CTGGCCCCTTCTCAGAGAGCTCTGGCACCAGCAGCCCCAGCCCATCCCCCAGAGCCTCCAACCACTCCCT CCACCCCAGCAGCTCTGCCTCCAGGGCCGGCACCAGCCCCTCTCTGGAGCAGGATGATGAGGATGAGGAA ACCAGAATGGTGATTGTTGGGAAAATTTCATTCTGCCCCAAGGATGTCCTGGGTCATGGAGCTGAGGGCA CAATTGTATACAAAGGTATGTTTGACAACCGAGATGTGGCCGTGAAGAGGATCCTCCCTGAGTGTTTTAG CTTTGCCGACCGTGAGGTCCAGCTGCTTCGAGAATCAGACGAGCACCCAAATGTGATCCGCTACTTTTGC ACAGAGAAGGACCGGCAGTTCCAGTACATTGCTATCGAGCTGTGTGCAGCCACCCTACAAGAGTATGTGG AGCAGAAGGACTTTGCCCACCTTGGCCTCGAGCCCATCACCCTGCTTCATCAGACCACCTCAGGCCTGGC ACACCTGCATTCTCTCAACATTGTTCACAGAGACCTGAAGCCCCACAACATTCTCCTCTCCATGCCCAAC GCACATGGCAGGATCAAGGCGATGATCTCTGACTTTGGCCTCTGCAAGAAGCTGGCAGTGGGCAGGCACA GTTTCAGCCGCCGTTCAGGGGTACCTGGCACTGAAGGGTGGATCGCCCCAGAGATGCTGAGTGAAGACTG TAAGGACAACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGTGTCTTTTACTATGTCATCTCTGAG GGCAACCATCCTTTTGGCAAATCCTTGCAGCGGCAGGCCAACATCCTCCTGGGCGCCTGCAACCTTGACT GTTTCCACTCAGACAAGCATGAGGACGTCATTGCTCGTGAATTGATAGAGAAAATGATTGCTATGGATCC CCAGCAGCGTCCCTCTGCAAAGCACGTGCTGAAACACCCCTTCTTCTGGAGCCTGGAGAAGCAGCTCCAG TTTTTCCAGGATGTAAGTGACCGAATAGAAAAGGAGGCCTTGGACGGTCCAATCGTACGGCAGTTGGAGA GAGGCGGGAGAGCTGTGGTCAAGATGGACTGGCGGGAGAACATCACTGTCCCCCTGCAGACAGATCTGCG CAAATTCAGAACCTACAAAGGTGGCTCTGTGAGAGACCTCCTCCGAGCCATGAGAAACAAGAAACACCAC TACCGGGAGCTCCCCGTGGAGGTTCAGGAGACGCTGGGCTCCATCCCGGATGACTTTGTGCGCTACTTCA CTTCCCGCTTCCCCCACCTCCTCTCTCACACCTACCAAGCCATGGAGCTGTGCAGACATGAGAGACTCTT TCAGACCTACTACTGGCACGAGCCCACAGAACCCCAGCCTCCAGTGATTCCATATGCCCTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_023913 |
| Insert Size | 2934 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_023913.2, NP_076402.1 |
| RefSeq Size | 3976 bp |
| RefSeq ORF | 2934 bp |
| Locus ID | 78943 |
| UniProt ID | Q9EQY0 |
| Gene Summary | Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR) (PubMed:11850408, PubMed:25164867). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain and subsequent activation of the endoribonuclease activity (PubMed:25164867). The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA (PubMed:11850408, PubMed:25164867). The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes (PubMed:11850408, PubMed:25164867). Acts as an upstream signal for ER stress-induced GORASP2-mediated unconventional (ER/Golgi-independent) trafficking of CFTR to cell membrane by modulating the expression and localization of SEC16A (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG226999 | Ern1 (tGFP-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1), (10ug) |
CNY 9,410.00 |
|
| MR226999 | Ern1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1) |
CNY 7,152.00 |
|
| MR226999L3 | Lenti ORF clone of Ern1 (Myc-DDK-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1) |
CNY 9,552.00 |
|
| MR226999L4 | Lenti ORF clone of Ern1 (mGFP-tagged) - Mouse endoplasmic reticulum (ER) to nucleus signalling 1 (Ern1) |
CNY 9,552.00 |
