Flt3 (NM_010229) Mouse Untagged Clone

CAT#: MC223148

Flt3 (untagged) - Mouse FMS-like tyrosine kinase 3 (Flt3), (10ug)



  "NM_010229" in other vectors (4)

CNY 6,968.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms B230315G04; CD135; Flk-2; Flk2; Flt-3; Ly72; wmfl
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223148 representing NM_010229
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGCGTTGGCGCAGCGCAGCGACCGGCGGCTGCTGCTGCTTGTTGTTTTGTCAGTAATGATTCTTG
AGACCGTTACAAACCAAGACCTGCCTGTGATCAAGTGTGTTTTAATCAGTCATGAGAACAATGGCTCATC
AGCGGGAAAGCCATCATCGTACCGAATGGTGCGAGGATCCCCAGAAGACCTCCAGTGTGCCCCGAGGCGC
CAGAGTGAAGGGACGGTATATGAAGCGGCCACCGTGGAGGTGGCCGAGTCTGGGTCCATCACCCTGCAAG
TGCAGCTCGCCACCCCAGGGGACCTTTCCTGCCTCTGGGTCTTTAAGCACAGCTCCCTGGGCTGCCAGCC
GCACTTTGATTTACAAAACAGAGGAATCGTTTCCATGGCCATCTTGAACGTGACAGAGACCCAGGCAGGA
GAATACCTACTCCATATTCAGAGCGAAGCCGCCAACTACACAGTACTGTTCACAGTGAATGTAAGAGATA
CACAGCTGTACGTGCTAAGAAGACCTTACTTTAGGAAGATGGAAAACCAGGACGCACTGCTCTGCATCTC
CGAGGGTGTTCCAGAGCCCACTGTGGAGTGGGTGCTCTGCAGCTCCCACAGGGAAAGCTGTAAAGAAGAA
GGCCCTGCTGTTGTCAGAAAGGAGGAAAAGGTACTTCATGAGTTGTTCGGAACAGACATCAGATGCTGTG
CTAGAAATGCACTGGGCCGCGAATGCACCAAGCTGTTCACCATAGATCTAAACCAGGCTCCTCAGAGCAC
ACTGCCCCAGTTATTCCTGAAAGTGGGGGAACCCTTGTGGATCAGGTGTAAGGCCATCCATGTGAACCAT
GGATTCGGGCTCACCTGGGAGCTGGAAGACAAAGCCCTGGAGGAGGGCAGCTACTTTGAGATGAGTACCT
ACTCCACAAACAGGACCATGATTCGGATTCTCTTGGCCTTTGTGTCTTCCGTGGGAAGGAACGACACCGG
ATATTACACCTGCTCTTCCTCAAAGCACCCCAGCCAGTCAGCGTTGGTGACCATCCTAGAAAAAGGGTTT
ATAAACGCTACCAGCTCGCAAGAAGAGTATGAAATTGACCCGTACGAAAAGTTCTGCTTCTCAGTCAGGT
TTAAAGCGTACCCACGAATCCGATGCACGTGGATCTTCTCTCAAGCCTCATTTCCTTGTGAACAGAGAGG
CCTGGAGGATGGGTACAGCATATCTAAATTTTGCGATCATAAGAACAAGCCAGGAGAGTACATATTCTAT
GCAGAAAATGATGACGCCCAGTTCACCAAAATGTTCACGCTGAATATAAGAAAGAAACCTCAAGTGCTAG
CAAATGCCTCAGCCAGCCAGGCGTCCTGTTCCTCTGATGGCTACCCGCTACCCTCTTGGACCTGGAAGAA
GTGTTCGGACAAATCTCCCAATTGCACGGAGGAAATCCCAGAAGGAGTTTGGAATAAAAAGGCTAACAGA
AAAGTGTTTGGCCAGTGGGTGTCGAGCAGTACTCTAAATATGAGTGAGGCCGGGAAAGGGCTTCTGGTCA
AATGCTGTGCGTACAATTCTATGGGCACGTCTTGCGAAACCATCTTTTTAAACTCACCAGGCCCCTTCCC
TTTCATCCAAGACAACATCTCCTTCTATGCGACCATTGGGCTCTGTCTCCCCTTCATTGTTGTTCTCATT
GTGTTGATCTGCCACAAATACAAAAAGCAATTTAGGTACGAGAGTCAGCTGCAGATGATCCAGGTGACTG
GCCCCCTGGATAACGAGTACTTCTACGTTGACTTCAGGGACTATGAATATGACCTTAAGTGGGAGTTCCC
GAGAGAGAACTTAGAGTTTGGGAAGGTCCTGGGGTCTGGCGCTTTCGGGAGGGTGATGAACGCCACGGCC
TATGGCATTAGTAAAACGGGAGTCTCAATTCAGGTGGCGGTGAAGATGCTAAAAGAGAAAGCTGACAGCT
GTGAAAAAGAAGCTCTCATGTCGGAGCTCAAAATGATGACCCACCTGGGACACCATGACAACATCGTGAA
TCTGCTGGGGGCATGCACACTGTCAGGGCCAGTGTACTTGATTTTTGAATATTGTTGCTATGGTGACCTC
CTCAACTACCTAAGAAGTAAAAGAGAGAAGTTTCACAGGACATGGACAGAGATTTTTAAGGAACATAATT
TCAGTTTTTACCCTACTTTCCAGGCACATTCAAATTCCAGCATGCCTGGTTCACGAGAAGTTCAGTTACA
CCCGCCCTTGGATCAGCTCTCAGGGTTCAATGGGAATTTAATTCATTCTGAAGATGAGATTGAATATGAA
AACCAGAAGAGGCTGGCAGAAGAAGAGGAGGAAGATTTGAACGTGCTGACGTTTGAAGACCTCCTTTGCT
TTGCGTACCAAGTGGCCAAAGGCATGGAATTCCTGGAGTTCAAGTCGTGTGTCCACAGAGACCTGGCAGC
CAGGAATGTGTTGGTCACCCACGGGAAGGTGGTGAAGATCTGTGACTTTGGACTGGCCCGAGACATCCTG
AGCGACTCCAGCTACGTCGTCAGGGGCAACGCACGGCTGCCGGTGAAGTGGATGGCACCTGAGAGCTTAT
TTGAAGGGATCTACACAATCAAGAGTGACGTCTGGTCCTACGGCATCCTTCTCTGGGAGATATTTTCACT
GGGTGTGAACCCTTACCCTGGCATTCCTGTCGACGCTAACTTCTATAAACTGATTCAGAGTGGATTTAAA
ATGGAGCAGCCATTCTATGCCACAGAAGGGATATACTTTGTAATGCAATCCTGCTGGGCTTTTGACTCAA
GGAAGCGGCCATCCTTCCCCAACCTGACTTCATTTTTAGGATGTCAGCTGGCAGAGGCAGAAGAAGCGAT
GTATCAGAACATGGGTGGCAACGTCCCAGAACATCCATCCATCTACCAAAACAGGCGGCCCCTCAGCAGA
GAGGCAGGCTCAGAGCCGCCATCGCCACAGGCCCAGGTGAAGATTCACGGAGAAAGAAGTTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_010229
Insert Size 3003 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_010229.2, NP_034359.2
RefSeq Size 3664 bp
RefSeq ORF 3003 bp
Locus ID 14255
UniProt ID Q00342
Gene Summary Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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