Myo1g (NM_178440) Mouse Untagged Clone

CAT#: MC223256

Myo1g (untagged) - Mouse myosin IG (Myo1g), (10ug)



  "NM_178440" in other vectors (4)

CNY 13,210.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms E430002D17Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC223256 representing NM_178440
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTGCAGTGGGCAGGATGGAGGACGAAGAAGGCCCTGAGTATGGGAAACCAGACTTCGTGCTTTTGG
ATCAACTGACCATGGAGGACTTCATGAAGAACCTAGAGCTCAGGTTTGAGAAGGGCCGTATCTATACCTA
CATTGGTGAGGTGCTCGTATCCGTGAACCCCTACCAGGAACTGCCATTGTATGGGCCAGAGGCCATTGCC
AAGTACCAGGGCCGCGAGCTCTATGAGCGACCACCTCATCTTTACGCCGTGGCCAATGCTGCTTACAAGG
CAATGAAGCGCAGATCCAGGGACACCTGCATCGTCATCTCAGGGGAGAGTGGGGCAGGGAAGACAGAAGC
CAGCAAGCACATCATGCAGTACATTGCTGCTGTCACCAACCCAAGCCAGAGGGCTGAGGTGGAGAGGGTG
AAGAATGTGCTCCTCAAGTCCACCTGTGTGCTCGAAGCCTTTGGCAATGCCCGCACCAATCGCAACCACA
ACTCCAGCCGCTTTGGCAAGTACATGGACATCAACTTCGACTTCAAGGGGGATCCTGTTGGTGGACACAT
CCACAGCTACCTGCTGGAGAAGTCTAGGGTTCTCAAGCAACATGTAGGTGAGAGGAACTTCCATGCCTTC
TACCAGTTGCTTCGAGGCAGTGAGGACCAAGAGCTGCAAGGACTGCATCTGGAAAGAAATCCTGCTGTGT
ATAATTTCACGCGTCAGGGAGCTGGGCTCAACATGGGTGTGCACAATGCCTTGGACAGTGATGAGAAGAG
CCACCAAGGAGTGATGGAGGCCATGAGGATCATCGGCTTCAGTCCTGACGAGGTGGAGTCCATCCATCGC
ATCCTTGCCGCCATATTACACCTGGGAAACATCGAGTTTGTGGAGACAGAGGAAAATGGACCACAGAAAG
GAGGCCTGGAAGTGGCTGATGAGGCCCTGGTAGGATATGTGGCCAAGCTGACAGCCACTCCCAGAGACCT
TGTTCTGCGAACCCTGCTGGCTCGAACAGTGGCTTCAGGAGGCCGAGAAGTCATTGAGAAGAGCCACACC
GTGGCTGAGGCCAGCTATGCCCGGGATGCCTGTGCAAAGGCGATGTACCAGCGACTGTTTGAGTGGGTTG
TGAACAAGATCAATAGCATCATGGAACCCCGAAACCGAGACCCTCGGTGTGATGGCAAGGATACTGTCAT
TGGGGTGCTGGACATTTACGGCTTTGAGGTGTTCCCTGTCAACAGCTTTGAGCAGTTCTGCATCAACTAC
TGCAACGAGAAGCTGCAGCAGCTCTTTATCCAGCTTATCCTGAAGCAAGAGCAGGAGGAGTATGAGCGAG
AGGGCATCGCCTGGCAGACCATCGAGTACTTCAACAACGCTACCATTGTGGAACTTGTAGAGCAGCCCCA
CAGAGGCATCCTGGCTGTGTTAGATGAAGCCTGCAGCACGGCAGGCCCCATCACTGACCGAATCTTCCTG
CAGACCCTGGACACACACCACCGCCACCACCCACACTATTCCAGCCGCCAGCTTTGCCCTACGGACAAGA
CCATGGAGTTTGGCCGAGACTTCCAGATCAAACACTATGCAGGCGATGTCACGTACTCTGTGGAAGGCTT
CATTGACAAGAATAGAGACTCTCTCTTCCAGGACTTCAAACGGCTGCTGTACAATAGTGTGGATCCCACC
TTGCGAGCCATGTGGCCTGACGGGCAACAGGACATCACGGAAGTGACCAAGCGTCCCCTGACAGCCGGCA
CACTCTTTAAGAATTCCATGGTTGCCCTGGTGGAAAACTTGGCTTCCAAGGAACCCTTCTATGTCCGCTG
CATCAAACCCAACGAAGACAAGGTGGCTGGGCGGCTCGATGAAGCCCACTGTCGTCACCAGGTCGAATAC
CTGGGACTATTGGAGAATGTGAGGGTCCGCAGGGCTGGCTTTGCTTCCCGGCAGCCCTACCCTCGATTCC
TGCTCAGGTACAAGATGACCTGTGAGTACACGTGGCCCAACCACCTGCTGGGCTCTGACCGGGATGCGGT
GAGCGCCCTGCTGGAACAGCATGGGCTGCAAGGGGATGTGGCCTTTGGCCACAGCAAGCTGTTCATCCGA
TCCCCAAGGACGCTGGTCACTCTGGAGCAGAGCCGAGCTCGCCTGATTCCCATCATTGTGTTATTGCTGC
AGAAGGCTTGGCGGGGCACCCTGGCTAGGTGGCACTGCCGGCGACTAAGGGCCATCTACACCATCATGCG
CTGGTTCCGGAGGCACAAGGTGCGTGCTCACCTGATTGAACTACAGCGCCGGTTCCAGGCTGCACGGCAG
CCCCCACTCTATGGCCGTGACCTTGTGTGGCCCACACCTCCTGCTGTGCTGCAGCCCTTCCAGGACACTT
GCCGTGTTCTCTTCAGCAGGTGGCGGGCACGGCAGTTAGTGAAGAACATCCCTCCTTCAGACATGATCCA
GATCAAGGCCAAGGTGGCTGCTATGGGGGCCTTGCAAGGATTGCGGCAGGACTGGGGTTGCCAGCGGGCC
TGGGCCCGAGACTACCTGTCCTCTGACACTGACAACCCCACAGCTTCCCATCTGTTTGCTGAGCAACTAA
AGGCACTTCGGGAGAAAGATGGCTTTGGCTCTGTGCTTTTCTCCAGCCATGTGCGCAAGGTGAATCGCTT
CCGCAAGAGCCGGGACCGGGCCCTTCTGCTCACAGATCGGTATCTGTACAAGCTGGAGCCTGGACGACAG
TACCGGGTGATGCGGGCTGTGCCTCTGGAGGCGGTGACAGGGCTGAGTGTGACCAGTGGAAGAGATCAGC
TGGTGGTGTTACATGCCCAAGGCTATGATGATCTTGTAGTGTGTCTACACCGTTCCCAACCACCACTGGA
CAATCGAATTGGGGAGCTGGTGGGCATGCTGGCTGCACACTGCCAGGGAGAGGGACGAACTCTGGAGGTC
CGTGTCTCTGACTGCATCCCACTGAGCCAGCGTGGTGCCCGGCGCCTCATTTCTGTGGAGCCCAGGCCAG
AGCAGCCTGAGCCAGATTTCCAAAGCAGCCGTAGCACCTTTACCCTCCTCTGGCCAAGCCACTGA


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-RsrII     
ACCN NM_178440
Insert Size 3075 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_178440.4, NP_848534.2
RefSeq Size 3307 bp
RefSeq ORF 3075 bp
Locus ID 246177
UniProt ID Q5SUA5
Gene Summary Unconventional myosin required during immune response for detection of rare antigen-presenting cells by regulating T-cell migration (PubMed:25083865). Unconventional myosins are actin-based motor molecules with ATPase activity and serve in intracellular movements. Acts as a regulator of T-cell migration by generating membrane tension, enforcing cell-intrinsic meandering search, thereby enhancing detection of rare antigens during lymph-node surveillance, enabling pathogen eradication (PubMed:25083865). Also required in B-cells, where it regulates different membrane/cytoskeleton-dependent processes (PubMed:24310084). Involved in Fc-gamma receptor (Fc-gamma-R) phagocytosis (PubMed:23038771).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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