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Myo1g (NM_178440) Mouse Tagged ORF Clone
CAT＃: MR219032
- TrueORF®
Myo1g (Myc-DDK-tagged) - Mouse myosin IG (Myo1g)
ORF Plasmid: tGFP
"NM_178440" in other vectors (4)
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CNY 7,136.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | E430002D17Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219032 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTTGCAGTGGGCAGGATGGAGGACGAAGAAGGCCCTGAGTATGGGAAACCAGACTTCGTGCTTTTGG ATCAACTGACCATGGAGGACTTCATGGAGAACCTAGAGCTCAGGTTTGAGAAGGGCCGTATCTATACCTA CATTGGTGAGGTGCTCGTATCCGTGAACCCCTACCAGGAACTGCCATTGTATGGGCCAGAGGCCATTGCC AAGTACCAGGGCCGCGAGCTCTATGAGCGACCACCTCATCTTTACGCCGTGGCCAATGCTGCTTACAAGG CAATGAAGCGCAGATCCAGGGACACCTGCATCGTCATCTCAGGGGAGAGTGGGGCAGGGAAGACAGAAGC CAGCAAGCACATCATGCAGTACATTGCTGCTGTCACCAACCCAAGCCAGAGGGCTGAGGTGGAGAGGGTG AAGAATGTGCTCCTCAAGTCCACCTGCGTGCTCGAAGCCTTTGGCAATGCCCGCACCAATCGCAACCACA ACTCCAGCCGCTTTGGCAAGTACATGGACATCAACTTCGACTTCAAGGGGGATCCTGTTGGTGGACACAT CCACAGCTACCTGCTGGAGAAGTCTAGGGTTCTCAAGCAACATGTAGGCGAGAGGAACTTCCATGCCTTC TACCAGTTGCTTCGAGGCAGTGAGGACCAAGAGCTGCAAGGACTGCATCTGGAAAGAAATCCTGCTGTGT ATAATTTCACGCGTCAGGGAGCTGGGCTCAACATGGGTGTGCACAATGCCTTGGACAGTGATGAGAAGAG CCACCAAGGAGTGATGGAGGCCATGAGGATCATCGGCTTCAGTCCTGACGAGGTGGAGTCCATCCATCGC ATCCTTGCCGCCATATTACACCTGGGAAACATCGAGTTTGTGGAGACAGAGGAAAATGGACCACAGAAAG GAGGCCTGGAAGTGGCTGATGAGGCCCTGGTAGGATATGTGGCCAAGCTGACAGCCACTCCCAGAGACCT TGTTCTGCGAACCCTGCTGGCTCGAACAGTGGCTTCAGGAGGCCGAGAAGTCATTGAGAAGAGCCACACC GTGGCTGAGGCCAGCTATGCCCGGGATGCCTGTGCAAAGGCGATGTACCAGCGACTGTTTGAGTGGGTTG TGAACAAGATCAATAGCATCATGGAACCCCGAAACCGAGACCCTCGGTGTGATGGCAAGGATACTGTCAT TGGGGTGCTGGACATTTACGGCTTTGAGGTGTTCCCTGTCAACAGCTTTGAGCAGTTCTGCATCAACTAC TGCAATGAGAAGCTGCAGCAGCTCTTTATCCAGCTTATCCTGAAGCAAGAGCAGGAGGAGTATGAGCGAG AGGGCATCGCCTGGCAGACCATCGAGTACTTCAACAACGCTACCATTGTGGAACTTGTAGAGCAGCCCCG CAGAGGCATCCTGGCTGTGTTAGATGAAGCCTGCAGCACGGCAGGCCCCATCACTGACCGAATCTTCCTG CAGACCCTGGACACACACCACCGCCACCACCCACACTATTCCAGCCGCCAGCTTTGCCCTACGGACAAGA CCATGGAGTTTGGCCGAGACTTCCAGATCAAACACTATGCAGGCGATGTCACGTACTCTGTGGAAGGCTT CATTGACAAGAATAGAGACTCTCTCTTCCAGGACTTCAAACGGCTGCTGTACAATAGTGTGGATCCCACC TTGCGAGCCATGTGGCCTGACGGGCAACAGGACATCACGGAAGTGACCAAGCGTCCCCTGACAGCCGGCA CACTCTTTAAGAATTCCATGGTTGCCCTGGTGGAAAACTTGGCTTCCAAGGAACCCTTCTATGTCCGCTG CATCAAACCCAACGAAGACAAGGTGGCTGGGCGGCTCGATGAAGCCCACTGTCGTCACCAGGTCGAATAC CTGGGACTATTGGAGAATGTGAGGGTCCGCAGGGCTGGCTTTGCTTCCCGGCAGCCCTACCCTCGATTCC TGCTCAGGTACAAGATGACCTGTGAGTACACGTGGCCCAACCACCTGCTGGGCTCTGACCGGGATGCGGT GAGCGCCCTGCTGGAACAGCATGGGCTGCAAGGGGATGTGGCCTTTGGCCACAGCAAGCTGTTCATCCGA TCCCCAAGGACGCTGGTCACTCTGGAGCAGAGCCGAGCTCGCCTGATTCCCATCATTGTGTTATTGCTGC AGAAGGCTTGGCGGGGCACCCTGGCTAGGTGGCACTGCCGGCGACTAAGGGCCATCTACACCATCATGCG CTGGTTCCGGAGGCACAAGGTGCGTGCTCACCTGATTGAACTACAGCGCCGGTTCCAGGCTGCACGGCAG CCCCCACTCTATGGCCGTGACCTTGTGTGGCCCACACCTCCTGCTGTGCTGCAGCCCTTCCAGGACACTT GCCGTGTTCTCTTCAGCAGGTGGCGGGCACGGCAGTTAGTGAAGAACATCCCTCCTTCAGACATGACCCA GATCAAGGCCAAGGTGGCTGCTATGGGGGCCTTGCAAGGATTGCGGCAGGACTGGGGTTGCCAGCGGGCC TGGGCCCGAGACTACCTGTCCTCTGACACTGACAACCCCACAGCTTCCCATCTGTTTGCTGAGCAACTAA AGGCACTTCGGGAGAAAGATGGCTTTGGCTCTGTGCTTTTCTCCAGCCATGTGCGCAAGGTGAATCGCTT CCGCAAGAGCCGGGACCGGGCCCTTCTGCTCACAGATCGGTATCTGTACAAGCTGGAGCCTGGACGACAG TACCGGGTGATGCGGGCTGTGCCTCTGGAGGCGGTGACAGGGCTGAGTGTGACCAGTGGAAGAGATCAGC TGGTGGTGTTACATGCCCAAGGCTATGATGATCTTGTAGTGTGTCTACACCGTTCCCAACCACCACTGGA CAATCGAATTGGGGAGCTGGTGGGCATGCTGGCTGCACACTGCCAGGGAGAGGGACGAACTCTGGAGGTC CGTGTCTCTGACTGCATCCCACTGAGCCAGCGTGGTGCCCGGCGCCTCATTTCTGTGGAGCCCAGGCCAG AGCAGCCTGAGCCAGATTTCCAAAGCAGCCGTAGCACCTTTACCCTCCTCTGGCCAAGCCAC AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR219032 protein sequence
Red=Cloning site Green=Tags(s) MLAVGRMEDEEGPEYGKPDFVLLDQLTMEDFMENLELRFEKGRIYTYIGEVLVSVNPYQELPLYGPEAIA KYQGRELYERPPHLYAVANAAYKAMKRRSRDTCIVISGESGAGKTEASKHIMQYIAAVTNPSQRAEVERV KNVLLKSTCVLEAFGNARTNRNHNSSRFGKYMDINFDFKGDPVGGHIHSYLLEKSRVLKQHVGERNFHAF YQLLRGSEDQELQGLHLERNPAVYNFTRQGAGLNMGVHNALDSDEKSHQGVMEAMRIIGFSPDEVESIHR ILAAILHLGNIEFVETEENGPQKGGLEVADEALVGYVAKLTATPRDLVLRTLLARTVASGGREVIEKSHT VAEASYARDACAKAMYQRLFEWVVNKINSIMEPRNRDPRCDGKDTVIGVLDIYGFEVFPVNSFEQFCINY CNEKLQQLFIQLILKQEQEEYEREGIAWQTIEYFNNATIVELVEQPRRGILAVLDEACSTAGPITDRIFL QTLDTHHRHHPHYSSRQLCPTDKTMEFGRDFQIKHYAGDVTYSVEGFIDKNRDSLFQDFKRLLYNSVDPT LRAMWPDGQQDITEVTKRPLTAGTLFKNSMVALVENLASKEPFYVRCIKPNEDKVAGRLDEAHCRHQVEY LGLLENVRVRRAGFASRQPYPRFLLRYKMTCEYTWPNHLLGSDRDAVSALLEQHGLQGDVAFGHSKLFIR SPRTLVTLEQSRARLIPIIVLLLQKAWRGTLARWHCRRLRAIYTIMRWFRRHKVRAHLIELQRRFQAARQ PPLYGRDLVWPTPPAVLQPFQDTCRVLFSRWRARQLVKNIPPSDMTQIKAKVAAMGALQGLRQDWGCQRA WARDYLSSDTDNPTASHLFAEQLKALREKDGFGSVLFSSHVRKVNRFRKSRDRALLLTDRYLYKLEPGRQ YRVMRAVPLEAVTGLSVTSGRDQLVVLHAQGYDDLVVCLHRSQPPLDNRIGELVGMLAAHCQGEGRTLEV RVSDCIPLSQRGARRLISVEPRPEQPEPDFQSSRSTFTLLWPSH SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178440 |
| ORF Size | 3075 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_178440.1, NM_178440.2, NM_178440.3, NM_178440.4, NP_848534.2 |
| RefSeq Size | 3307 bp |
| RefSeq ORF | 3075 bp |
| Locus ID | 246177 |
| UniProt ID | Q5SUA5 |
| MW | 117.2 kDa |
| Gene Summary | Unconventional myosin required during immune response for detection of rare antigen-presenting cells by regulating T-cell migration (PubMed:25083865). Unconventional myosins are actin-based motor molecules with ATPase activity and serve in intracellular movements. Acts as a regulator of T-cell migration by generating membrane tension, enforcing cell-intrinsic meandering search, thereby enhancing detection of rare antigens during lymph-node surveillance, enabling pathogen eradication (PubMed:25083865). Also required in B-cells, where it regulates different membrane/cytoskeleton-dependent processes (PubMed:24310084). Involved in Fc-gamma receptor (Fc-gamma-R) phagocytosis (PubMed:23038771).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223256 | Myo1g (untagged) - Mouse myosin IG (Myo1g), (10ug) |
CNY 13,210.00 |
|
| MG219032 | Myo1g (tGFP-tagged) - Mouse myosin IG (Myo1g), (10ug) |
CNY 9,220.00 |
|
| MR219032L3 | Lenti ORF clone of Myo1g (Myc-DDK-tagged) - Mouse myosin IG (Myo1g) |
CNY 10,260.00 |
|
| MR219032L4 | Lenti ORF clone of Myo1g (mGFP-tagged) - Mouse myosin IG (Myo1g) |
CNY 10,260.00 |
