Flnb (NM_134080) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AL024016; Fln-b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225370 representing NM_134080 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGACGGAGAAAGACCTGGCGGAGGACGCGCCGTGGAAGAAGATTCAGCAGAACACGTTCACGC GCTGGTGCAACGAGCACCTCAAGTGTGTGAATAAACGCATCGGGAACCTGCAGACCGACCTGAGCGACGG GCTACGGCTCATCGCGCTGCTCGAGGTGCTCAGCCAGAAGCGCATGCACCACAAGTACCATCAGCGGCCC ACCTTCAGACAGATGAAGCTGGAGAATGTGTCTGTGGCTCTGGAGTTCCTAGACCACGAAAGCATTAAGC TCGTGTCCATTGACAGTAAAGCCATCGTGGATGGGAACCTGAAGCTCATCTTGGGCCTGGTATGGACTTT GATCCTTCACTACTCCATCTCCATGCCCGTATGGGAAGATGAAGGGGATGATGATGCCAAGAAGCAGACA CCAAAGCAGAGGCTACTGGGATGGATTCAGAACAAGATTCCATACTTGCCCATCACTAACTTTAACCAGA ACTGGCAAGATGGCAAAGCCTTAGGAGCCCTGGTAGACAGCTGTGCTCCAGGTCTGTGCCCAGACTGGGA ATCCTGGGACCCACGGAAGCCGGTGGATAATGCCCGAGAAGCCATGCAGCAGGCCGATGACTGGCTGGGC GTCCCACAGGTCATCACTCCTGAGGAGATTATTCACCCGGACGTGGACGAGCACTCTGTGATGACCTACC TGTCTCAGTTCCCCAAAGCCAAGCTCAAGCCAGGTGCTCCTCTTAAACCCAAACTCAACCCAAAGAAAGC CCGAGCCTATGGCAGAGGAATCGAACCTACTGGAAACATGGTGAAGCAGCCAGCCAAATTCACGGTAGAT ACCATCAGCGCTGGACAAGGAGATGTGATGGTGTTTGTTGAGGACCCAGAAGGAAACAAGGAAGAGGCAC GTGTGACCCCAGACAGTGACAAGAACAAGACATACTCTGTGGAGTATCTGCCCAAAGTCACCGGCCTGCA TAAAGTCATCGTCCTCTTTGCAGGACAGCACATTTCTAAGAGCCCATTTGAAGTGAATGTTGACAAAGCC CAAGGAGATGCCAGCAAAGTCACTGCAAAAGGCCCAGGGCTGGAAACTACAGGGAACATTGCCAACAAGC CTACCTATTTTGACATCTACACAGCTGGAGCTGGCGTGGGGGACATCGGCATTGAGGTGGAGGATCCCCA GGGCAAGAACAGCGTGGAGCTGCTGGTGGAAGACAGAGGAAACCAGGTGTATAGATGTGTCTACAAACCA GTGCAGCCTGGCCCCCATGTGGTCAAGGTCTCCTTTGCTGGGGATGCCATTCCCAAGAGTCCCTTTGGTG TACAAATTGGGGAAGCCTGCAACCCAAATGCCTGCAGAGCCAGCGGCCGAGGTCTGCAGCCCAAAGGTGT CCGGATCCGGGAGACTGCAGACTTTAAGGTGGACACCAAGGCTGCTGGAAGCGGAGAGCTTGGTGTAACT GTGAAGGGCCCTAAGGGCCTGGAGGAGCTGGTCAAACAAAAGGGCTTTCTGGATGGGGTCTACTCATTTG AGTACTACCCCAGCACACCAGGGAAATACAGCGTTGCAGTTACCTGGGGGGGGCACCACATTCCCAAGAG CCCCTTTGAAGTTCAAGTTGGCCCTGAAGCAGGCATGCAGAAAGTCCGTGCATGGGGCCCTGGACTCCAC GGCGGCATTGTTGGTCGGTCAGCAGATTTTGTGGTGGAATCCATTGGTTCTGAGGTTGGAACTCTGGGAT TTGCCATTGAAGGCCCTTCCCAAGCAAAGATCGAGTATGATGATCAGAACGATGGCTCGTGTGATGTCAA ATACTGGCCCAAGGAGCCTGGAGAATACGCCGTTCACATCATGTGCGACGACGAAGACATCAAGGACAGC CCATACATGGCTTTCATCCACCCTGCCACAGGAGACTACAACCCAGATCTAGTCCAAGCCTATGGGCCGG GTTTGGAGAAATCTGGTTGTACTATCAACAACCCAGCAGAGTTCATTGTGGATCCTAAGGATGCCGGGAG TGCTCCCTTAAAGATACTGGCGCAGGATGGGGAAGGCCAGCCCATTGACATCCAGATGAAGAGTCGGATG GACGGCACTTATGCTTGCTCATATACCCCACTTAAAGCCATCAAGCACACCATCGCTGTGGTCTGGGGAG GTGTGAACATACCACACAGCCCCTACAGGGTCAACATTGGGCAAGGTAGCCATCCCCAAAAGGTCAAAGT GTTTGGCCCAGGCGTGGAGAGGAGTGGTCTGAAGGCAAATGAGCCTACTCACTTTACAGTGGATTGCACG GAGGCCGGGGAAGGTGATGTCAGTGTTGGTATCAAGTGCGATGCCCGAGTTTTAAGTGACGATGAGGAAG ATGTCGATTTTGACATTATTCACAATGCCAATGACACATTCACTGTCAAATACGTACCTCCTGCCCCTGG GCGGTATACCATCAAAGTTCTCTTTGCATCTCAGGAAATCCCCGCCAGCCCTTTCAGAGTCAAAGTTGAC CCTTCCCATGACGCCAGCAAGGTGAAGGCAGAAGGCCCCGGGTTGAGTAAAGCAGGCGTGGAAAATGGGA AGCCAACCCACTTCACTGTTCACACCAAGGGAGCCGGAAAAGCTCCGCTCAACGTGCAGTTCAGCAGCCC CCTTCCTGGGGAGGCAGTGAAGGACTTGGATATCATTGATAATTATGACTACTCACATACTGTTAAATAC ACCCCAACCCAGCAGGGCAACATGCAGGTTCTAGTGACTTATGGTGGCGATCCCATTCCTAAAAGTCCTT TCACTGTGGGCGTTGCTGCGCCACTGGATCTGAGCAAGATAAAAATTAATGGACTGGAAAACAGAGTTGA GGTTGGAAAGGACCAGGAATTTGCCATTGACACCAACGGGGCAGGAGGCCAGGGGAAGCTGGATGTGACC ATCCTGAGCCCTTCTCGGAAGGTGGTGCCATGCTTGGTAGCACCAGTGGCTGGCAGGGAATGCAGCACAG CCAAATTCATCCCTCGGGAGGAGGGGCTGTTTGCTGTAGATGTGACCTATGATGGACACCCAGTGCCCGG GAGCCCCTATACTGTGGAGGCCTCACTACCACCAGATCCCACCAAGGTGAAGGCCCACGGTCCTGGCCTT GAAGGAGGTCTTGTAGGCAAGCCCGCTGAGTTCACCATTGACACTAAAGGAGCTGGAACTGGAGGTTTGG GCCTAACTGTGGAAGGTCCATGTGAGGCCAAAATCGAATGCTCGGATAACGGTGACGGGACCTGCTCCGT CTCCTACCTTCCCACAAAACCCGGAGAATACTTTGTCAACATTCTCTTTGAAGAAGTCCACATTCCTGGA TCTCCCTTCAAAGCCGACATTGAAATGCCGTTTGACCCCTCTAAGGTTGTAGCGTCAGGACCAGGACTTG AACACGGAAAAGTGGGTGAACCCGGAATCCTTTGCGTTGATTGTTCAGAAGCAGGGCCTGGGACTCTGGG CCTTGAAGCCGTCTCAGACTCAGGGGCGAAAGCTGAAGTGTCCATCCAGAACAATAAAGATGGCACCTAC GCTGTGACCTATGTGCCTCTGACAGCTGGCATGTACACACTGACCATGAAGTATGGTGGTGAGCTTGTAC CACACTTTCCTGCCTGGGTCAAGGTGGAGCCAGCCATAGACACTAGTGGGATCAAAGCCTTTGGGCCAGG AATCGAAGGCAAGGACGTGTTTCGGGAGGCCACAACGGACTTCACGGTTGACTCGAGGCCCCTTACCCAG GTTGGAGGTGACCATATCAAAGCTCAAATTACCAACCCCTCGGGGGCCTCCACCGAGTGCTTTGTCAAGG ACAATGCTGATGGGACCTATCAGGTCGAATACACGCCCTTTGAGAAAGGTTTCCATGTAGTGGAGGTGAC ATACGATGATGTGCCCATCCCAAATAGCCCATTCAAAGTGGCTGTCACTGAAGGCTGCCAGCCATCTCGG GTCCATGCGCAAGGCCCTGGACTGAAAGAGGCGTTTACCAACAAATCTAATGTCTTCACAGTGGTAACCA GGGGAGCAGGGATCGGTGGGCTTGGCATAACTGTGGAGGGGCCGTCGGAGTCAAAGATAAACTGCAGAGA CAACAAGGATGGCAGCTGCAGTGCTGAGTACATTCCTTTCGCTCCAGGGGATTATGACGTCAATATCACG TACGGAGGCGTCCACATCCCTGGCAGCCCCTTCAGAGTTCCTTCAAAAGATGTTGTGGATCCCAGCAAGG TCAAGATTGCTGGCCCCGGTCTGAGCTCCTGTGTCCGAGCATGCATCCCGCAGTCCTTCACAGTGGACAG CAGCAAGGCTGGCCTAGCTCCCCTGGAAGTGAGGGTCCTGGGCCCAAGAGGCCTCGTGGAGCCGGTGAAT GTGGTAGATAATGGGGACGGCACCCACACAGTGACCTACACGCCATCTCAGGAGGGGCCCTACATTGTTT CCGTCAAATATGCTGATGAAGAGATCCCCCGAAGTCCCTTTAAGGTCAAGGTTCTTCCCACATACGATGC AAGCAAAGTGACAGCCTCTGGGCCTGGCCTCAGCGCCTATGGGGTACCCGCCAGCCTACCTGTGGAGTTC GCCATTGATGCCAGAGATGCTGGCGAAGGCCTGCTTGCTGTCCAGATCACGGACCAGGAGGGAAAGCCTC AAAGAGCTACTGTCCACGACAACAAAGATGGCACATATGCCGTTACCTATATCCCTGATAAGACCGGACG CTATATGATTGGAGTCACCTATGGTGGAGACAACATTCCACTTTCTCCTTACCGTATCCGGGCCACACAG ACAGGCGATGCCAGCAAGTGCTTGGCCACAGGTCCTGGTATTGCCCCCACTGTGAAAACTGGCGAGGAGG TGGGCTTTGTAGTGGACGCTAAGACTGCAGGGAAGGGGAAAGTAACCTGCGTGATTCTGACCCCAGATGG CACCGAGGCTGAGGCTGACGTCATTGAAAATGAAGATGGCACCTATGACATTTTCTACACTGCTGCCAAG CCAGGCACCTATGTTATCTATGTGCGCTTTGGTGGCGTTGATATTCCCAATAGCCCCTTCACAGTCATGG CCACCGATGGGGAAGTCACAGCCATGGAGGAGGCACCGGTTACAGAAGAGGCCTATGTCCCTGTGAGTGA CATGAACGGGCTAGGCTTCAAGCCCTTTGACTTGGTGATTCCATTTGCCGTCAGGAAAGGAGAAATTACA GGCACGGTGCATATGCCTTCTGGGAAGAAGGCTACACCTGAGATTGTGGACAACAAGGACGGCACGGTCA CTGTGAGATACGCGCCCACTGAAGTCGGCCTCCACGAGATGCACATCAAGTACAGGGGCAGCCACATCCC GGAGAGCCCCCTGCAGTTCTACGTGAACTACCCCAACAGTGGGAGTGTGTCTGCTTATGGTCCAGGCCTT GTGTATGGAGTAGCCAACAAAACTGCCACCTTCACCATTGTCACAGAGGATGCGGGAGAAGGTGGTCTGG ACTTGGCTATTGAAGGACCCTCGAAAGCAGAGATCAGCTGCATTGACAACAAAGACGGGACATGTACAGT GACCTACCTACCCACCTTGCCAGGGGACTACAGCATTCTGGTCAAGTACAATGACAAACATATTCCTGGC AGCCCCTTCACTGCCAAGATCACAGATGATAACAGACGCTGCTCCCAAGTGAAGCTGGGCTCAGCTGCTG ACTTCCTTCTGGACATCAGTGAGACAGACCTTAGCACACTGACAGCCAGTATCAAGGCCCCATCTGGACG TGACGAGCCCTGTCTGCTAAAGAGGCTACCCAACAACCACATTGGCATTTCCTTCATCCCCCGAGAAGTG GGTGAGCATCTAGTCAGCATCAAGAAAAACGGTAACCACGTGGCCAACAGCCCTGTGTCCATTATGGTGG TCCAGTCTGAAATTGGTGATGCACGCCGAGCCAAAGTCTATGGCCAAGGCCTGTCAGAAGGCCGGACTTT TGAGATGTCAGACTTCATCGTGGACACAAGAGATGCAGGCTATGGTGGCATATCCTTGGCAGTAGAAGGA CCCAGCAAGGTGGACATCCAAACAGAGGACCTGGAGGACGGCACCTGCAAGGTGTCCTACTTCCCCACTG TGCCCGGGGTCTATATCGTCTCCACCAAATTTGCTGACGAGCATGTGCCTGGAAGCCCATTCACTGTGAA GATCAGTGGCGAGGGAAGAGTCAGAGAGAGCATCACCCGGACCAGCCGGGCTCCGGCTGTGGCCACGGTT GGCAGTATCTGTGACCTGAACCTGAAGATCCCGGAGATCAACAGCAGTGACATGTCTGCCCATGTCACCA GCCCCTCTGGCCATGTCACTGAGGCAGAGATTGTGCCCATGGGAAAGAACTCTCACTGTGTCAGATTTGT GCCCCAGGAGATGGGCGTTCACACTGTCAGCGTAAAGTACCGCGGACAGCACGTAACAGGAAGCCCGTTC CAGTTCACCGTGGGGCCCCTTGGCGAAGGGGGTGCCCACAAGGTCCGGGCAGGAGGCCCTGGCCTGGAGA GGGGAGAAGCAGGCATCCCAGCTGAGTTCAGCATCTGGACCCGGGAAGCAGGTGCTGGCGGCCTCTCCAT TGCTGTTGAGGGCCCTAGTAAGGCCGAGATCACATTCGATGACCATAAAAATGGGTCATGCGGTGTGTCT TATATTGCCCAAGAGCCTGGTAACTACGAGGTGTCTATCAAGTTCAACGACGAGCACATCCCGGACAGCC CTTACCTGGTGCCCGTCATCGCGCCCTCAGACGACGCTCGCTGCCTCACTGTTCTGAGCCTTCAGGAATC AGGATTAAAAGTTAACCAGCCAGCGTCCTTTGCTATAAGGTTGAATGGTGCGAAAGGGAAGATTGATGCA AAGGTGCACAGCCCCTCAGGAGCCGTGGAGGAATGCCACGTGTCTGAACTGGAGCCAGATAAGTATGCCG TTCGCTTCATCCCGCATGAGAATGGCATCCACACGATCGATGTCAAGTTTAACGGGAGCCACGTGGTCGG GAGCCCTTTCAAAGTACGTGTAGGAGAGCCTGGCCAGGCAGGCAATCCTGCTCTAGTGTCAGCCTACGGT GCTGGGCTTGAGACGGGCACCACAGGTATCCAATCAGAATTCTTCATCAATACCACTCAGGCAGGCCCAG GGACATTATCTGTCACCATTGAAGGACCATCCAAGGTCAAAATGGATTGCCAGGAAATCCCAGAGGGGTA CAAAGTCATGTACACTCCCATGGCTCCTGGAAATTACCTGATTGGTGTCAAATATGGCGGGCCCAACCAC ATCTCGAGGAGTCCATTCAAGGCCAAGGTGACAGGCCAACGTCTGGTCAGCCCAGGCTCAGCCAACGAGA CCTCCTCCATCTTGGTGGAGTCCGTAACCAGATCCTCAACGGAAACGTGCTATAGCGCCATTCCCAAGTC GTCCTCCGATGCCAGCAAAGTGACCTCCAAGGGCGCAGGCCTGTCAAAGGCTTTTGTGGGCCAGAAGAGT TCTTTCCTGGTGGACTGCAGCAAAGCTGGGTCCAACATGCTGTTGATTGGGGTCCATGGGCCCACCACCC CCTGTGAAGAGGTCTCCATGAAGCATGTGGGCAAGCAACAGTACAACGTCACCTACGTCGTCAAGGAGAG GGGCGATTACGTTCTGGCTGTGAAGTGGGGGGAAGAGCACATCCCCGGTAGCCCCTTTCATGTCACCGTG CCTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_134080
Insert Size	7776 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_134080.1, NP_598841.1
RefSeq Size	9083 bp
RefSeq ORF	7776 bp
Locus ID	286940
Gene Summary	Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. This gene lacks full-length transcript support, and therefore the exon combination of this variant is inferred based on tiled partial transcripts and full-length orthologous alignments, e.g., human AB191258.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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