Flnb (NM_134080) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AL024016; Fln-b
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR219210 representing NM_134080 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGACGGAGAAAGACCTGGCGGAGGACGCGCCGTGGAAGAAGATTCAGCAGAACACGTTCACGC GCTGGTGCAACGAGCACCTCAAGTGTGTGAATAAACGCATCGGGAACCTGCAGACCGACCTGAGCGACGG GCTACGGCTCATCGCGCTGCTCGAGGTGCTCAGCCAGAAGCGCATGCACCACAAGTACCATCAGCGGCCC ACCTTCAGACAGATGAAGCTGGAGAATGTGTCTGTGGCTCTGGAGTTCCTAGACCACGAAAGCATTAAGC TCGTGTCCATTGACAGTAAAGCCATCGTGGATGGGAACCTGAAGCTCATCTTGGGCCTGGTATGGACTTT GATCCTTCACTACTCCATCTCCATGCCCGTATGGGAAGATGAAGGGGATGATGATGCCAAGAAGCAGACA CCAAAGCAGAGGCTACTGGGATGGATTCAGAACAAGATTCCATACTTGCCCATCACTAACTTTAACCAGA ACTGGCAAGATGGCAAAGCCTTAGGAGCCCTGGTAGACAGCTGTGCTCCAGGTCTGTGCCCAGACTGGGA ATCCTGGGACCCACGGAAGCCGGTGGATAATGCCCGAGAAGCCATGCAGCAGGCCGATGACTGGCTGGGC GTCCCACAGGTCATCACTCCTGAGGAGATTATTCACCCGGACGTGGACGAGCACTCTGTGATGACCTACC TGTCTCAGTTCCCCAAAGCCAAGCTCAAGCCAGGTGCTCCTCTTAAACCCAAACTCAACCCAAAGAAAGC CCGAGCCTATGGCAGAGGAATCGAACCTACTGGAAACATGGTGAAGCAGCCAGCCAAATTCACGGTAGAT ACCATCAGCGCTGGACAAGGAGATGTGATGGTGTTTGTTGAGGACCCAGAAGGAAACAAGGAAGAGGCAC GTGTGACCCCAGACAGTGACAAGAACAAGACATACTCTGTGGAGTATCTGCCCAAAGTCACCGGCCTGCA TAAAGTCATCGTCCTCTTTGCAGGACAGCACATTTCTAAGAGCCCATTTGAAGTGAATGTTGACAAAGCC CAAGGAGATGCCAGCAAAGTCACTGCAAAAGGCCCAGGGCTGGAAACTACAGGGAACATTGCCAACAAGC CTACCTATTTTGACATCTACACAGCTGGAGCTGGCGTGGGGGACATCGGCATTGAGGTGGAGGATCCCCA GGGCAAGAACAGCGTGGAGCTGCTGGTGGAAGACAGAGGAAACCAGGTGTATAGATGTGTCTACAAACCA GTGCAGCCTGGCCCCCATGTGGTCAAGGTCTCCTTTGCTGGGGATGCCATTCCCAAGAGTCCCTTTGGTG TACAAATTGGGGAAGCCTGCAACCCAAATGCCTGCAGAGCCAGCGGCCGAGGTCTGCAGCCCAAAGGTGT CCGGATCCGGGAGACTGCAGACTTTAAGGTGGACACCAAGGCTGCTGGAAGCGGAGAGCTTGGTGTAACT GTGAAGGGCCCTAAGGGCCTGGAGGAGCTGGTCAAACAAAAGGGCTTTCTGGATGGGGTCTACTCATTTG AGTACTACCCCAGCACACCAGGGAAATACAGCGTTGCAGTTACCTGGGGGGGGCACCACATTCCCAAGAG CCCCTTTGAAGTTCAAGTTGGCCCTGAAGCAGGCATGCAGAAAGTCCGTGCATGGGGCCCTGGACTCCAC GGCGGCATTGTTGGTCGGTCAGCAGATTTTGTGGTGGAATCCATTGGTTCTGAGGTTGGAACTCTGGGAT TTGCCATTGAAGGCCCTTCCCAAGCAAAGATCGAGTATGATGATCAGAACGATGGCTCGTGTGATGTCAA ATACTGGCCCAAGGAGCCTGGAGAATACGCCGTTCACATCATGTGCGACGACGAAGACATCAAGGACAGC CCATACATGGCTTTCATCCACCCTGCCACAGGAGACTACAACCCAGATCTAGTCCAAGCCTATGGGCCGG GTTTGGAGAAATCTGGTTGTACTATCAACAACCCAGCAGAGTTCATTGTGGATCCTAAGGATGCCGGGAG TGCTCCCTTAAAGATACTGGCGCAGGATGGGGAAGGCCAGCCCATTGACATCCAGATGAAGAGTCGGATG GACGGCACTTATGCTTGCTCATATACCCCACTTAAAGCCATCAAGCACACCATCGCTGTGGTCTGGGGAG GTGTGAACATACCACACAGCCCCTACAGGGTCAACATTGGGCAAGGTAGCCATCCCCAAAAGGTCAAAGT GTTTGGCCCAGGCGTGGAGAGGAGTGGTCTGAAGGCAAATGAGCCTACTCACTTTACAGTGGATTGCACG GAGGCCGGGGAAGGTGATGTCAGTGTTGGTATCAAGTGCGATGCCCGAGTTTTAAGTGACGATGAGGAAG ATGTCGATTTTGACATTATTCACAATGCCAATGACACATTCACTGTCAAATACGTACCTCCTGCCCCTGG GCGGTATACCATCAAAGTTCTCTTTGCATCTCAGGAAATCCCCGCCAGCCCTTTCAGAGTCAAAGTTGAC CCTTCCCATGACGCCAGCAAGGTGAAGGCAGAAGGCCCCGGGTTGAGTAAAGCAGGCGTGGAAAATGGGA AGCCAACCCACTTCACTGTTCACACCAAGGGAGCCGGAAAAGCTCCGCTCAACGTGCAGTTCAGCAGCCC CCTTCCTGGGGAGGCAGTGAAGGACTTGGATATCATTGATAATTATGACTACTCACATACTGTTAAATAC ACCCCAACCCAGCAGGGCAACATGCAGGTTCTAGTGACTTATGGTGGCGATCCCATTCCTAAAAGTCCTT TCACTGTGGGCGTTGCTGCGCCACTGGATCTGAGCAAGATAAAAATTAATGGACTGGAAAACAGAGTTGA GGTTGGAAAGGACCAGGAATTTGCCATTGACACCAACGGGGCAGGAGGCCAGGGGAAGCTGGATGTGACC ATCCTGAGCCCTTCTCGGAAGGTGGTGCCATGCTTGGTAGCACCAGTGGCTGGCAGGGAATGCAGCACAG CCAAATTCATCCCTCGGGAGGAGGGGCTGTTTGCTGTAGATGTGACCTATGATGGACACCCAGTGCCCGG GAGCCCCTATACTGTGGAGGCCTCACTACCACCAGATCCCACCAAGGTGAAGGCCCACGGTCCTGGCCTT GAAGGAGGTCTTGTAGGCAAGCCCGCTGAGTTCACCATTGACACTAAAGGAGCTGGAACTGGAGGTTTGG GCCTAACTGTGGAAGGTCCATGTGAGGCCAAAATCGAATGCTCGGATAACGGTGACGGGACCTGCTCCGT CTCCTACCTTCCCACAAAACCCGGAGAATACTTTGTCAACATTCTCTTTGAAGAAGTCCACATTCCTGGA TCTCCCTTCAAAGCCGACATTGAAATGCCGTTTGACCCCTCTAAGGTTGTAGCGTCAGGACCAGGACTTG AACACGGAAAAGTGGGTGAACCCGGAATCCTTTGCGTTGATTGTTCAGAAGCAGGGCCTGGGACTCTGGG CCTTGAAGCCGTCTCAGACTCAGGGGCGAAAGCTGAAGTGTCCATCCAGAACAATAAAGATGGCACCTAC GCTGTGACCTATGTGCCTCTGACAGCTGGCATGTACACACTGACCATGAAGTATGGTGGTGAGCTTGTAC CACACTTTCCTGCCTGGGTCAAGGTGGAGCCAGCCATAGACACTAGTGGGATCAAAGCCTTTGGGCCAGG AATCGAAGGCAAGGACGTGTTTCGGGAGGCCACAACGGACTTCACGGTTGACTCGAGGCCCCTTACCCAG GTTGGAGGTGACCATATCAAAGCTCAAATTACCAACCCCTCGGGGGCCTCCACCGAGTGCTTTGTCAAGG ACAATGCTGATGGGACCTATCAGGTCGAATACACGCCCTTTGAGAAAGGTTTCCATGTAGTGGAGGTGAC ATACGATGATGTGCCCATCCCAAATAGCCCATTCAAAGTGGCTGTCACTGAAGGCTGCCAGCCATCTCGG GTCCATGCGCAAGGCCCTGGACTGAAAGAGGCGTTTACCAACAAATCTAATGTCTTCACAGTGGTAACCA GGGGAGCAGGGATCGGTGGGCTTGGCATAACTGTGGAGGGGCCGTCGGAGTCAAAGATAAACTGCAGAGA CAACAAGGATGGCAGCTGCAGTGCTGAGTACATTCCTTTCGCTCCAGGGGATTATGACGTCAATATCACG TACGGAGGCGTCCACATCCCTGGCAGCCCCTTCAGAGTTCCTTCAAAAGATGTTGTGGATCCCAGCAAGG TCAAGATTGCTGGCCCCGGTCTGAGCTCCTGTGTCCGAGCATGCATCCCGCAGTCCTTCACAGTGGACAG CAGCAAGGCTGGCCTAGCTCCCCTGGAAGTGAGGGTCCTGGGCCCAAGAGGCCTCGTGGAGCCGGTGAAT GTGGTAGATAATGGGGACGGCACCCACACAGTGACCTACACGCCATCTCAGGAGGGGCCCTACATTGTTT CCGTCAAATATGCTGATGAAGAGATCCCCCGAAGTCCCTTTAAGGTCAAGGTTCTTCCCACATACGATGC AAGCAAAGTGACAGCCTCTGGGCCTGGCCTCAGCGCCTATGGGGTACCCGCCAGCCTACCTGTGGAGTTC GCCATTGATGCCAGAGATGCTGGCGAAGGCCTGCTTGCTGTCCAGATCACGGACCAGGAGGGAAAGCCTC AAAGAGCTACTGTCCACGACAACAAAGATGGCACATATGCCGTTACCTATATCCCTGATAAGACCGGACG CTATATGATTGGAGTCACCTATGGTGGAGACAACATTCCACTTTCTCCTTACCGTATCCGGGCCACACAG ACAGGCGATGCCAGCAAGTGCTTGGCCACAGGTCCTGGTATTGCCCCCACTGTGAAAACTGGCGAGGAGG TGGGCTTTGTAGTGGACGCTAAGACTGCAGGGAAGGGGAAAGTAACCTGCGTGATTCTGACCCCAGATGG CACCGAGGCTGAGGCTGACGTCATTGAAAATGAAGATGGCACCTATGACATTTTCTACACTGCTGCCAAG CCAGGCACCTATGTTATCTATGTGCGCTTTGGTGGCGTTGATATTCCCAATAGCCCCTTCACAGTCATGG CCACCGATGGGGAAGTCACAGCCATGGAGGAGGCACCGGTTACAGAAGAGGCCTATGTCCCTGTGAGTGA CATGAACGGGCTAGGCTTCAAGCCCTTTGACTTGGTGATTCCATTTGCCGTCAGGAAAGGAGAAATTACA GGCACGGTGCATATGCCTTCTGGGAAGAAGGCTACACCTGAGATTGTGGACAACAAGGACGGCACGGTCA CTGTGAGATACGCGCCCACTGAAGTCGGCCTCCACGAGATGCACATCAAGTACAGGGGCAGCCACATCCC GGAGAGCCCCCTGCAGTTCTACGTGAACTACCCCAACAGTGGGAGTGTGTCTGCTTATGGTCCAGGCCTT GTGTATGGAGTAGCCAACAAAACTGCCACCTTCACCATTGTCACAGAGGATGCGGGAGAAGGTGGTCTGG ACTTGGCTATTGAAGGACCCTCGAAAGCAGAGATCAGCTGCATTGACAACAAAGACGGGACATGTACAGT GACCTACCTACCCACCTTGCCAGGGGACTACAGCATTCTGGTCAAGTACAATGACAAACATATTCCTGGC AGCCCCTTCACTGCCAAGATCACAGATGATAACAGACGCTGCTCCCAAGTGAAGCTGGGCTCAGCTGCTG ACTTCCTTCTGGACATCAGTGAGACAGACCTTAGCACACTGACAGCCAGTATCAAGGCCCCATCTGGACG TGACGAGCCCTGTCTGCTAAAGAGGCTACCCAACAACCACATTGGCATTTCCTTCATCCCCCGAGAAGTG GGTGAGCATCTAGTCAGCATCAAGAAAAACGGTAACCACGTGGCCAACAGCCCTGTGTCCATTATGGTGG TCCAGTCTGAAATTGGTGATGCACGCCGAGCCAAAGTCTATGGCCAAGGCCTGTCAGAAGGCCGGACTTT TGAGATGTCAGACTTCATCGTGGACACAAGAGATGCAGGCTATGGTGGCATATCCTTGGCAGTAGAAGGA CCCAGCAAGGTGGACATCCAAACAGAGGACCTGGAGGACGGCACCTGCAAGGTGTCCTACTTCCCCACTG TGCCCGGGGTCTATATCGTCTCCACCAAATTTGCTGACGAGCATGTGCCTGGAAGCCCATTCACTGTGAA GATCAGTGGCGAGGGAAGAGTCAGAGAGAGCATCACCCGGACCAGCCGGGCTCCGGCTGTGGCCACGGTT GGCAGTATCTGTGACCTGAACCTGAAGATCCCGGAGATCAACAGCAGTGACATGTCTGCCCATGTCACCA GCCCCTCTGGCCATGTCACTGAGGCAGAGATTGTGCCCATGGGAAAGAACTCTCACTGTGTCAGATTTGT GCCCCAGGAGATGGGCGTTCACACTGTCAGCGTAAAGTACCGCGGACAGCACGTAACAGGAAGCCCGTTC CAGTTCACCGTGGGGCCCCTTGGCGAAGGGGGTGCCCACAAGGTCCGGGCAGGAGGCCCTGGCCTGGAGA GGGGAGAAGCAGGCATCCCAGCTGAGTTCAGCATCTGGACCCGGGAAGCAGGTGCTGGCGGCCTCTCCAT TGCTGTTGAGGGCCCTAGTAAGGCCGAGATCACATTCGATGACCATAAAAATGGGTCATGCGGTGTGTCT TATATTGCCCAAGAGCCTGGTAACTACGAGGTGTCTATCAAGTTCAACGACGAGCACATCCCGGACAGCC CTTACCTGGTGCCCGTCATCGCGCCCTCAGACGACGCTCGCTGCCTCACTGTTCTGAGCCTTCAGGAATC AGGATTAAAAGTTAACCAGCCAGCGTCCTTTGCTATAAGGTTGAATGGTGCGAAAGGGAAGATTGATGCA AAGGTGCACAGCCCCTCAGGAGCCGTGGAGGAATGCCACGTGTCTGAACTGGAGCCAGATAAGTATGCCG TTCGCTTCATCCCGCATGAGAATGGCATCCACACGATCGATGTCAAGTTTAACGGGAGCCACGTGGTCGG GAGCCCTTTCAAAGTACGTGTAGGAGAGCCTGGCCAGGCAGGCAATCCTGCTCTAGTGTCAGCCTACGGT GCTGGGCTTGAGACGGGCACCACAGGTATCCAATCAGAATTCTTCATCAATACCACTCAGGCAGGCCCAG GGACATTATCTGTCACCATTGAAGGACCATCCAAGGTCAAAATGGATTGCCAGGAAATCCCAGAGGGGTA CAAAGTCATGTACACTCCCATGGCTCCTGGAAATTACCTGATTGGTGTCAAATATGGCGGGCCCAACCAC ATCTCGAGGAGTCCATTCAAGGCCAAGGTGACAGGCCAACGTCTGGTCAGCCCAGGCTCAGCCAACGAGA CCTCCTCCATCTTGGTGGAGTCCGTAACCAGATCCTCAACGGAAACGTGCTATAGCGCCATTCCCAAGTC GTCCTCCGATGCCAGCAAAGTGACCTCCAAGGGCGCAGGCCTGTCAAAGGCTTTTGTGGGCCAGAAGAGT TCTTTCCTGGTGGACTGCAGCAAAGCTGGGTCCAACATGCTGTTGATTGGGGTCCATGGGCCCACCACCC CCTGTGAAGAGGTCTCCATGAAGCATGTGGGCAAGCAACAGTACAACGTCACCTACGTCGTCAAGGAGAG GGGCGATTACGTTCTGGCTGTGAAGTGGGGGGAAGAGCACATCCCCGGTAGCCCCTTTCATGTCACCGTG CCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR219210 representing NM_134080 Red=Cloning site Green=Tags(s) MPVTEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIGNLQTDLSDGLRLIALLEVLSQKRMHHKYHQRP TFRQMKLENVSVALEFLDHESIKLVSIDSKAIVDGNLKLILGLVWTLILHYSISMPVWEDEGDDDAKKQT PKQRLLGWIQNKIPYLPITNFNQNWQDGKALGALVDSCAPGLCPDWESWDPRKPVDNAREAMQQADDWLG VPQVITPEEIIHPDVDEHSVMTYLSQFPKAKLKPGAPLKPKLNPKKARAYGRGIEPTGNMVKQPAKFTVD TISAGQGDVMVFVEDPEGNKEEARVTPDSDKNKTYSVEYLPKVTGLHKVIVLFAGQHISKSPFEVNVDKA QGDASKVTAKGPGLETTGNIANKPTYFDIYTAGAGVGDIGIEVEDPQGKNSVELLVEDRGNQVYRCVYKP VQPGPHVVKVSFAGDAIPKSPFGVQIGEACNPNACRASGRGLQPKGVRIRETADFKVDTKAAGSGELGVT VKGPKGLEELVKQKGFLDGVYSFEYYPSTPGKYSVAVTWGGHHIPKSPFEVQVGPEAGMQKVRAWGPGLH GGIVGRSADFVVESIGSEVGTLGFAIEGPSQAKIEYDDQNDGSCDVKYWPKEPGEYAVHIMCDDEDIKDS PYMAFIHPATGDYNPDLVQAYGPGLEKSGCTINNPAEFIVDPKDAGSAPLKILAQDGEGQPIDIQMKSRM DGTYACSYTPLKAIKHTIAVVWGGVNIPHSPYRVNIGQGSHPQKVKVFGPGVERSGLKANEPTHFTVDCT EAGEGDVSVGIKCDARVLSDDEEDVDFDIIHNANDTFTVKYVPPAPGRYTIKVLFASQEIPASPFRVKVD PSHDASKVKAEGPGLSKAGVENGKPTHFTVHTKGAGKAPLNVQFSSPLPGEAVKDLDIIDNYDYSHTVKY TPTQQGNMQVLVTYGGDPIPKSPFTVGVAAPLDLSKIKINGLENRVEVGKDQEFAIDTNGAGGQGKLDVT ILSPSRKVVPCLVAPVAGRECSTAKFIPREEGLFAVDVTYDGHPVPGSPYTVEASLPPDPTKVKAHGPGL EGGLVGKPAEFTIDTKGAGTGGLGLTVEGPCEAKIECSDNGDGTCSVSYLPTKPGEYFVNILFEEVHIPG SPFKADIEMPFDPSKVVASGPGLEHGKVGEPGILCVDCSEAGPGTLGLEAVSDSGAKAEVSIQNNKDGTY AVTYVPLTAGMYTLTMKYGGELVPHFPAWVKVEPAIDTSGIKAFGPGIEGKDVFREATTDFTVDSRPLTQ VGGDHIKAQITNPSGASTECFVKDNADGTYQVEYTPFEKGFHVVEVTYDDVPIPNSPFKVAVTEGCQPSR VHAQGPGLKEAFTNKSNVFTVVTRGAGIGGLGITVEGPSESKINCRDNKDGSCSAEYIPFAPGDYDVNIT YGGVHIPGSPFRVPSKDVVDPSKVKIAGPGLSSCVRACIPQSFTVDSSKAGLAPLEVRVLGPRGLVEPVN VVDNGDGTHTVTYTPSQEGPYIVSVKYADEEIPRSPFKVKVLPTYDASKVTASGPGLSAYGVPASLPVEF AIDARDAGEGLLAVQITDQEGKPQRATVHDNKDGTYAVTYIPDKTGRYMIGVTYGGDNIPLSPYRIRATQ TGDASKCLATGPGIAPTVKTGEEVGFVVDAKTAGKGKVTCVILTPDGTEAEADVIENEDGTYDIFYTAAK PGTYVIYVRFGGVDIPNSPFTVMATDGEVTAMEEAPVTEEAYVPVSDMNGLGFKPFDLVIPFAVRKGEIT GTVHMPSGKKATPEIVDNKDGTVTVRYAPTEVGLHEMHIKYRGSHIPESPLQFYVNYPNSGSVSAYGPGL VYGVANKTATFTIVTEDAGEGGLDLAIEGPSKAEISCIDNKDGTCTVTYLPTLPGDYSILVKYNDKHIPG SPFTAKITDDNRRCSQVKLGSAADFLLDISETDLSTLTASIKAPSGRDEPCLLKRLPNNHIGISFIPREV GEHLVSIKKNGNHVANSPVSIMVVQSEIGDARRAKVYGQGLSEGRTFEMSDFIVDTRDAGYGGISLAVEG PSKVDIQTEDLEDGTCKVSYFPTVPGVYIVSTKFADEHVPGSPFTVKISGEGRVRESITRTSRAPAVATV GSICDLNLKIPEINSSDMSAHVTSPSGHVTEAEIVPMGKNSHCVRFVPQEMGVHTVSVKYRGQHVTGSPF QFTVGPLGEGGAHKVRAGGPGLERGEAGIPAEFSIWTREAGAGGLSIAVEGPSKAEITFDDHKNGSCGVS YIAQEPGNYEVSIKFNDEHIPDSPYLVPVIAPSDDARCLTVLSLQESGLKVNQPASFAIRLNGAKGKIDA KVHSPSGAVEECHVSELEPDKYAVRFIPHENGIHTIDVKFNGSHVVGSPFKVRVGEPGQAGNPALVSAYG AGLETGTTGIQSEFFINTTQAGPGTLSVTIEGPSKVKMDCQEIPEGYKVMYTPMAPGNYLIGVKYGGPNH ISRSPFKAKVTGQRLVSPGSANETSSILVESVTRSSTETCYSAIPKSSSDASKVTSKGAGLSKAFVGQKS SFLVDCSKAGSNMLLIGVHGPTTPCEEVSMKHVGKQQYNVTYVVKERGDYVLAVKWGEEHIPGSPFHVTV P TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_134080
ORF Size	7773 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_134080.1, NP_598841.1
RefSeq Size	9083 bp
RefSeq ORF	7776 bp
Locus ID	286940
MW	276.6 kDa
Gene Summary	Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton (By similarity).[UniProtKB/Swiss-Prot Function]

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