Ecel1 (NM_001277925) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	DINE; xce
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228914 representing NM_001277925 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCCCGTATTCCATGACAGCGCACTACGACGAGTTCCAGGAAGTCAAGTATGTGAGCCGGTGTG GCACCGGGGGCGCTCGAGGGACCTCACTGCCTCCAGGCTTCCCGAGGGGCTCAGGGCGCAGTGCTAGCGG GTCCAGGTCTGGGCTGCCCCGCTGGAATCGACGCGAGGTGTGCCTGCTGTCGGGGTTGGTGTTTGCTGCC GGCCTCTGCGCCATCCTGGCGGCCATGCTGGCGCTCAAATACCTGGGCCCGGGAGCGGCGGGCGGTGGCG GAGCCTGTCCCGAGGGCTGCCCCGAGCGCAAGGCCTTTGCACGCGCCGCCCGCTTTCTGTCTGCCAACCT GGACGCCAGCATCGACCCGTGCCAGGACTTCTACTCGTTCGCGTGCGGAGGCTGGCTGCGGCGCCACGCT ATCCCCGACGACAAGCTCACCTACGGCACGATCGCGGCCATCGGAGAGCAGAACGAGGAGCGTCTGCGGC GCCTGCTGGCGAGACCCACCGGAGGCCCCGGCGGCGCTGCGCAGCGCAAGGTGCGCGCCTTCTTCCGCTC GTGCCTCGACATGCGCGAGATCGAGAGGCTCGGGCCGCGGCCCATGCTCGAGGTCATCGAGGACTGCGGC GGCTGGGACCTGGGCGGCGCGGCCGATCGCCCCGGGGCGGCTCGCTGGGACCTCAACCGACTGCTGTACA AGGCACAGGGTGTGTACAGCGCGGCCGCGCTCTTCTCGCTTACAGTCAGCCTGGACGACAGGAACTCCTC GCGCTACGTCATCCGCATTGACCAGGATGGGCTCACCCTGCCAGAGAGAACCCTGTACCTAGCTCAAGAC GAGGAGAGTGAGAAGATCTTGGCTGCATACAGGGTGTTCATGCAGCGTCTACTCAGACTACTGGGAGCGG ACGCTGTGGAGCAGAAGGCGCAGGAAATCCTTCAGCTGGAACAGAGGCTGGCCAATATCTCTGTGTCAGA ATATGATGATCTCCGTCGTGATGTCAGCTCCGCGTACAACAAAGTGACTCTGGGGCAGCTGCAGAAGATC ATCCCCCATTTGCAGTGGAAGTGGCTGCTGGATCAGATCTTCCAGGAAGACTTCTCGGAGGAGGAGGAGG TGGTGCTGCTGGCCACAGACTACATGCAGCAGGTGTCCCAGCTCATCCGCTCCACACCTCGAAGGATCCT GCACAACTACCTAGTGTGGCGTGTGGTGGTGGTTCTGAGTGAGCACCTGTCCTCACCATTCCGAGAGGCA CTGCATGAGCTGGCCAAAGAGATGGAGGGCAATGACAAGCCCCAGGAGTTGGCCCGAGTCTGCCTGGGCC AGGCCAACCGCCACTTCGGCATGGCTCTTGGTGCCCTCTTTGTACATGAACACTTCTCAGCTGCCAGTAA AGCCAAGGTGCAGCAGCTAGTGGAAGATATCAAGTACATCTTGGGCCAGCGCCTGGAGGAGCTGGACTGG ATGGATGCCCAGACCAAGGCAGCTGCTCGGGCCAAGCTCCAGTACATGATGGTCATGGTTGGCTATCCAG ACTTCCTGCTGAAACCTGAGGCTGTAGACAAAGAATATGAGTTCGAGGTCCACGAGAAGACCTACTTCAA GAACATTTTGAACAGCATCCGATTCAGTATCCAGTTGTCCGTCAAAAAGATTCGACAGGAGGTGGACAAA TCCTCGTGGCTCCTCCCACCACAGGCGCTCAATGCCTACTATCTGCCCAACAAGAATCAAATGGTCTTCC CAGCTGGCATCCTGCAGCCCACCCTGTATGACCCAGACTTCCCACAGTCTCTGAACTACGGGGGTATTGG CACCATCATTGGGCACGAACTGACCCATGGCTATGACGACTGGGGGGGACAGTATGACCGCTCAGGAAAC CTGTTGCACTGGTGGACTGAGACCTCCTACAGCCACTTTCTGCGCAAGGCCGAGTGCATCGTTCGCCTCT ATGACAACTTCACCGTCTACAACCAGCGCGTGAATGGGAAACACACACTTGGCGAGAACATCGCAGACAT GGGAGGCCTCAAGCTGGCCTACTATGCCTATCAGAAGTGGGTCCGGGAGCATGGCCCGGAGCACCCGCTG CACCGGCTCAAGTACACACACAACCAGCTTTTCTTCATTGCCTTTGCACAGAACTGGTGCATCAAGCGAC GGTCACAGTCCATCTACCTGCAGGTGCTGACAGACAAGCATGCACCTGAGCACTACCGGGTCCTGGGCAG CGTATCCCAGTTTGAGGAATTCGGCCGGGCCTTCCACTGTCCCAAGGACTCTCCCATGAACCCCGTCCAT AAGTGCTCTGTGTGGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277925
Insert Size	2328 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277925.1, NP_001264854.1
RefSeq Size	2906 bp
RefSeq ORF	2328 bp
Locus ID	13599
UniProt ID	Q9JMI0
Gene Summary	May contribute to the degradation of peptide hormones and be involved in the inactivation of neuronal peptides.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.

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