Ecel1 (NM_001277925) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	DINE; xce
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231125 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCCCGTATTCCATGACAGCGCACTACGACGAGTTCCAGGAAGTCAAGTATGTGAGCCGGTGTG GCACCGGGGGCGCTCGAGGGACCTCACTGCCTCCAGGCTTCCCGAGGGGCTCAGGGCGCAGTGCTAGCGG GTCCAGGTCTGGGCTGCCCCGCTGGAATCGACGCGAGGTGTGCCTGCTGTCGGGGTTGGTGTTTGCTGCC GGCCTCTGCGCCATCCTGGCGGCCATGCTGGCGCTCAAATACCTGGGCCCGGGAGCGGCGGGCGGTGGCG GAGCCTGTCCCGAGGGCTGCCCCGAGCGCAAGGCCTTTGCACGCGCCGCCCGCTTTCTGTCTGCCAACCT GGACGCCAGCATCGACCCGTGCCAGGACTTCTACTCGTTCGCGTGCGGAGGCTGGCTGCGGCGCCACGCT ATCCCCGACGACAAGCTCACCTACGGCACGATCGCGGCCATCGGAGAGCAGAACGAGGAGCGTCTGCGGC GCCTGCTGGCGAGACCCACCGGAGGCCCCGGCGGCGCTGCGCAGCGCAAGGTGCGCGCCTTCTTCCGCTC GTGCCTCGACATGCGCGAGATCGAGAGGCTCGGGCCGCGGCCCATGCTCGAGGTCATCGAGGACTGCGGC GGCTGGGACCTGGGCGGCGCGGCCGATCGCCCCGGGGCGGCTCGCTGGGACCTCAACCGACTGCTGTACA AGGCACAGGGTGTGTACAGCGCGGCCGCGCTCTTCTCGCTTACAGTCAGCCTGGACGACAGGAACTCCTC GCGCTACGTCATCCGCATTGACCAGGATGGGCTCACCCTGCCAGAGAGAACCCTGTACCTAGCTCAAGAC GAGGAGAGTGAGAAGATCTTGGCTGCATACAGGGTGTTCATGCAGCGTCTACTCAGACTACTGGGAGCGG ACGCTGTGGAGCAGAAGGCGCAGGAAATCCTTCAGCTGGAACAGAGGCTGGCCAATATCTCTGTGTCAGA ATATGATGATCTCCGTCGTGATGTCAGCTCCGCGTACAACAAAGTGACTCTGGGGCAGCTGCAGAAGATC ATCCCCCATTTGCAGTGGAAGTGGCTGCTGGATCAGATCTTCCAGGAAGACTTCTCGGAGGAGGAGGAGG TGGTGCTGCTGGCCACAGACTACATGCAGCAGGTGTCCCAGCTCATCCGCTCCACACCTCGAAGGATCCT GCACAACTACCTAGTGTGGCGTGTGGTGGTGGTTCTGAGTGAGCACCTGTCCTCACCATTCCGAGAGGCA CTGCATGAGCTGGCCAAAGAGATGGAGGGCAATGACAAGCCCCAGGAGTTGGCCCGAGTCTGCCTGGGCC AGGCCAACCGCCACTTCGGCATGGCTCTTGGTGCCCTCTTTGTACATGAACACTTCTCAGCTGCCAGTAA AGCCAAGGTGCAGCAGCTAGTGGAAGATATCAAGTACATCTTGGGCCAGCGCCTGGAGGAGCTGGACTGG ATGGATGCCCAGACCAAGGCAGCTGCTCGGGCCAAGCTCCAGTACATGATGGTCATGGTTGGCTATCCAG ACTTCCTGCTGAAACCTGAGGCTGTAGACAAAGAATATGAGTTCGAGGTCCACGAGAAGACCTACTTCAA GAACATTTTGAACAGCATCCGATTCAGTATCCAGTTGTCCGTCAAAAAGATTCGACAGGAGGTGGACAAA TCCTCGTGGCTCCTCCCACCACAGGCGCTCAATGCCTACTATCTGCCCAACAAGAATCAAATGGTCTTCC CAGCTGGCATCCTGCAGCCCACCCTGTATGACCCAGACTTCCCACAGTCTCTGAACTACGGGGGTATTGG CACCATCATTGGGCACGAACTGACCCATGGCTATGACGACTGGGGGGGACAGTATGACCGCTCAGGAAAC CTGTTGCACTGGTGGACTGAGACCTCCTACAGCCACTTTCTGCGCAAGGCCGAGTGCATCGTTCGCCTCT ATGACAACTTCACCGTCTACAACCAGCGCGTGAATGGGAAACACACACTTGGCGAGAACATCGCAGACAT GGGAGGCCTCAAGCTGGCCTACTATGCCTATCAGAAGTGGGTCCGGGAGCATGGCCCGGAGCACCCGCTG CACCGGCTCAAGTACACACACAACCAGCTTTTCTTCATTGCCTTTGCACAGAACTGGTGCATCAAGCGAC GGTCACAGTCCATCTACCTGCAGGTGCTGACAGACAAGCATGCACCTGAGCACTACCGGGTCCTGGGCAG CGTATCCCAGTTTGAGGAATTCGGCCGGGCCTTCCACTGTCCCAAGGACTCTCCCATGAACCCCGTCCAT AAGTGCTCTGTGTGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231125 protein sequence Red=Cloning site Green=Tags(s) MEAPYSMTAHYDEFQEVKYVSRCGTGGARGTSLPPGFPRGSGRSASGSRSGLPRWNRREVCLLSGLVFAA GLCAILAAMLALKYLGPGAAGGGGACPEGCPERKAFARAARFLSANLDASIDPCQDFYSFACGGWLRRHA IPDDKLTYGTIAAIGEQNEERLRRLLARPTGGPGGAAQRKVRAFFRSCLDMREIERLGPRPMLEVIEDCG GWDLGGAADRPGAARWDLNRLLYKAQGVYSAAALFSLTVSLDDRNSSRYVIRIDQDGLTLPERTLYLAQD EESEKILAAYRVFMQRLLRLLGADAVEQKAQEILQLEQRLANISVSEYDDLRRDVSSAYNKVTLGQLQKI IPHLQWKWLLDQIFQEDFSEEEEVVLLATDYMQQVSQLIRSTPRRILHNYLVWRVVVVLSEHLSSPFREA LHELAKEMEGNDKPQELARVCLGQANRHFGMALGALFVHEHFSAASKAKVQQLVEDIKYILGQRLEELDW MDAQTKAAARAKLQYMMVMVGYPDFLLKPEAVDKEYEFEVHEKTYFKNILNSIRFSIQLSVKKIRQEVDK SSWLLPPQALNAYYLPNKNQMVFPAGILQPTLYDPDFPQSLNYGGIGTIIGHELTHGYDDWGGQYDRSGN LLHWWTETSYSHFLRKAECIVRLYDNFTVYNQRVNGKHTLGENIADMGGLKLAYYAYQKWVREHGPEHPL HRLKYTHNQLFFIAFAQNWCIKRRSQSIYLQVLTDKHAPEHYRVLGSVSQFEEFGRAFHCPKDSPMNPVH KCSVW TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001277925
ORF Size	2328 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001277925.1, NP_001264854.1
RefSeq Size	2906 bp
RefSeq ORF	2328 bp
Locus ID	13599
UniProt ID	Q9JMI0
MW	88 kDa
Gene Summary	May contribute to the degradation of peptide hormones and be involved in the inactivation of neuronal peptides.[UniProtKB/Swiss-Prot Function]

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