Ube2w (NM_025773) Mouse Tagged ORF Clone
CAT#: MG201089
- TrueORF®
Ube2w (tGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2W (putative) (Ube2w)
ORF Plasmid: DDK
"NM_025773" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 6130401J04Rik |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201089 representing NM_025773
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCAATGCAGAAACGACTACAAAAAGAACTGTTGGCTTTGCAGAATGACCCACCTCCTGGAATGA CTTTAAATGAAAAGAGTGTTCAGAATTCAATCACGCAGTGGATCGTAGACATGGAAGGTGCACCAGGAAC CTTATATGAAGGGGAAAAATTTCAACTTTTGTTTAAATTTAGTAGTCGATACCCTTTTGACTCTCCTCAG GTCATGTTTACTGGTGAAAATATTCCCATTCATCCTCATGTGTATAGCAATGGTCATATCTGTTTATCCA TTCTAACAGAAGACTGGTCCCCGGCGCTCTCAGTGCAGTCAGTCTGTCTCAGCATTATCAGCATGCTTTC CAGCTGCAAAGAAAAGAGACGACCACCAGATAATTCCTTTTATGTGCGAACATGTAACAAGAATCCAAAG AAAACAAAATGGTGGTATCATGATGACACTTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201089 representing NM_025773
Red=Cloning site Green=Tags(s) MASMQKRLQKELLALQNDPPPGMTLNEKSVQNSITQWIVDMEGAPGTLYEGEKFQLLFKFSSRYPFDSPQ VMFTGENIPIHPHVYSNGHICLSILTEDWSPALSVQSVCLSIISMLSSCKEKRRPPDNSFYVRTCNKNPK KTKWWYHDDTC TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025773 |
ORF Size | 453 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025773.4 |
RefSeq Size | 2355 bp |
RefSeq ORF | 543 bp |
Locus ID | 66799 |
UniProt ID | Q8VDW4 |
Gene Summary | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Specifically monoubiquitinates the N-terminus of various substrates, including ATXN3, MAPT/TAU, POLR2H/RPB8 and STUB1/CHIP, by recognizing backbone atoms of disordered N-termini (PubMed:21855799, PubMed:21229326). Involved in degradation of misfolded chaperone substrates by mediating monoubiquitination of STUB1/CHIP, leading to recruitment of ATXN3 to monoubiquitinated STUB1/CHIP, and restriction of the length of ubiquitin chain attached to STUB1/CHIP substrates by ATXN3 (PubMed:21855799). After UV irradiation, but not after mitomycin-C (MMC) treatment, acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed:21229326). In vitro catalyzes 'Lys-11'-linked polyubiquitination. UBE2W-catalyzed ubiquitination occurs also in the presence of inactive RING/U-box type E3s, i.e. lacking the active site cysteine residues to form thioester bonds with ubiquitin, or even in the absence of E3, albeit at a slower rate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207595 | Ube2w (untagged) - Mouse ubiquitin-conjugating enzyme E2W (putative) (Ube2w), (10ug) |
CNY 3,990.00 |
|
MR201089 | Ube2w (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2W (putative) (Ube2w) |
CNY 1,200.00 |
|
MR201089L3 | Lenti ORF clone of Ube2w (Myc-DDK-tagged) - Mouse ubiquitin-conjugating enzyme E2W (putative) (Ube2w) |
CNY 4,750.00 |
|
MR201089L4 | Lenti ORF clone of Ube2w (mGFP-tagged) - Mouse ubiquitin-conjugating enzyme E2W (putative) (Ube2w) |
CNY 4,750.00 |