Arl3 (NM_019718) Mouse Tagged ORF Clone
CAT#: MG201653
- TrueORF®
Arl3 (tGFP-tagged) - Mouse ADP-ribosylation factor-like 3 (Arl3)
ORF Plasmid: DDK
"NM_019718" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG201653 representing NM_019718
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTTGCTCTCTATTTTGCGCAAATTGAAAAGTGCACCAGACCAGGAGGTGCGAATCCTACTCCTGG GCTTGGACAACGCGGGCAAGACTACCTTGCTGAAGCAACTCGCATCTGAAGACATCAGCCACATCACACC TACACAGGGATTTAACATCAAAAGCGTGCAGTCACAAGGTTTTAAGCTGAATGTATGGGACATTGGCGGG CAGAGGAAAATCAGACCATACTGGAGAAGTTATTTTGAAAATACTGATATTCTCATCTATGTAATTGACA GTGCCGACAGAAAGAGATTTGAAGAGACGGGTCAGGAACTAACGGAATTACTGGAGGAAGAAAAGCTGAG TTGTGTGCCCGTGCTCATCTTTGCTAACAAGCAGGACCTGCTCACGGCAGCCCCAGCCTCCGAGATCGCA GAAGGGCTAAACCTCCACACCATCCGGGACCGCGTCTGGCAGATCCAGTCCTGTTCAGCGCTCACAGGCG AGGGCGTCCAGGATGGCATGAACTGGGTCTGCAAGAATGTCAACGCAAAGAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG201653 representing NM_019718
Red=Cloning site Green=Tags(s) MGLLSILRKLKSAPDQEVRILLLGLDNAGKTTLLKQLASEDISHITPTQGFNIKSVQSQGFKLNVWDIGG QRKIRPYWRSYFENTDILIYVIDSADRKRFEETGQELTELLEEEKLSCVPVLIFANKQDLLTAAPASEIA EGLNLHTIRDRVWQIQSCSALTGEGVQDGMNWVCKNVNAKKK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_019718 |
ORF Size | 546 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_019718.3 |
RefSeq Size | 838 bp |
RefSeq ORF | 549 bp |
Locus ID | 56350 |
UniProt ID | Q9WUL7 |
Gene Summary | Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP) (PubMed:18376416). Required for normal cytokinesis and cilia signaling. Required for targeting proteins to the cilium, including myristoylated NPHP3 and prenylated INPP5E. Targets NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium (By similarity). Requires assistance from GTPase-activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms (PubMed:15979089). Required for PKD1:PKD2 complex targeting from the trans-Golgi network to the cilium (PubMed:25405894).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC203496 | Arl3 (untagged) - Mouse ADP-ribosylation factor-like 3 (Arl3), (10ug) |
CNY 2,400.00 |
|
MR201653 | Arl3 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 3 (Arl3) |
CNY 2,400.00 |
|
MR201653L3 | Lenti ORF clone of Arl3 (Myc-DDK-tagged) - Mouse ADP-ribosylation factor-like 3 (Arl3) |
CNY 4,750.00 |
|
MR201653L4 | Lenti ORF clone of Arl3 (mGFP-tagged) - Mouse ADP-ribosylation factor-like 3 (Arl3) |
CNY 4,750.00 |