Ing4 (NM_133345) Mouse Tagged ORF Clone
CAT#: MG203150
- TrueORF®
Ing4 (tGFP-tagged) - Mouse inhibitor of growth family, member 4 (Ing4)
ORF Plasmid: DDK
"NM_133345" in other vectors (4)
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CNY 2,760.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | D6Wsu147e; D6Xrf92; p29ING4 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203150 representing NM_133345
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCTGGGATGTATTTGGAACATTATCTGGACAGTATTGAAAACCTCCCGTTTGAATTACAGAGAA ACTTTCAGCTCATGAGGGACCTAGACCAAAGGACAGAGGACCTGAAGGCTGAAATTGACAAGTTGGCCAC TGAATATATGAGTAGCGCCCGCAGCCTGAGCTCCGAGGAGAAGCTGGCCCTTCTCAGACAGATCCAGGAG GCCTATGGCAAGTGCAAGGAATTTGGTGACGACAAGGTGCAGCTGGCCATGCAGACCTATGAGATGGTAG ACAAACACATTCGGCGGCTGGACACAGACCTGGCCCGTTTTGAGGCTGATCTGAAGGAGAAACAGATCGA GTCCAGTGACTATGACAGCTCTTCTAGCAAAGGCAAAAAGAGCCGGACCCAAAAGGAGAAAAAAGCTGCC AGAGCCCGTTCCAAAGGGAAAAACTCAGATGAAGAAGCCCCCAAGGCTGCCCAGAAGAAGTTAAAACTTG TGCGCACAAGTCCTGAGTATGGGATGCCCTCAGTGACCTTTGGCAGTGTCCACCCCTCTGATGTGTTGGA TATGCCTGTGGATCCCAACGAACCCACATATTGCCTTTGTCACCAGGTCTCCTATGGAGAGATGATTGGC TGTGACAACCCGGATTGTTCCATCGAGTGGTTCCACTTCGCCTGTGTGGGGCTGACAACCAAACCTCGAG GGAAATGGTTTTGCCCACGCTGCTCCCAAGAACGGAAGAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203150 representing NM_133345
Red=Cloning site Green=Tags(s) MAAGMYLEHYLDSIENLPFELQRNFQLMRDLDQRTEDLKAEIDKLATEYMSSARSLSSEEKLALLRQIQE AYGKCKEFGDDKVQLAMQTYEMVDKHIRRLDTDLARFEADLKEKQIESSDYDSSSSKGKKSRTQKEKKAA RARSKGKNSDEEAPKAAQKKLKLVRTSPEYGMPSVTFGSVHPSDVLDMPVDPNEPTYCLCHQVSYGEMIG CDNPDCSIEWFHFACVGLTTKPRGKWFCPRCSQERKKK TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_133345 |
ORF Size | 744 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_133345.2 |
RefSeq Size | 1618 bp |
RefSeq ORF | 747 bp |
Locus ID | 28019 |
UniProt ID | Q8C0D7 |
Gene Summary | Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1) (By similarity). Can enhance apoptosis induced by serum starvation in mammary epithelial cell line HC11 (PubMed:11888890).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200711 | Ing4 (untagged) - Mouse inhibitor of growth family, member 4 (Ing4), (10ug) |
CNY 2,400.00 |
|
MR203150 | Ing4 (Myc-DDK-tagged) - Mouse inhibitor of growth family, member 4 (Ing4) |
CNY 2,470.00 |
|
MR203150L3 | Lenti ORF clone of Ing4 (Myc-DDK-tagged) - Mouse inhibitor of growth family, member 4 (Ing4) |
CNY 4,370.00 |
|
MR203150L4 | Lenti ORF clone of Ing4 (mGFP-tagged) - Mouse inhibitor of growth family, member 4 (Ing4) |
CNY 4,370.00 |