Elf5 (NM_010125) Mouse Tagged ORF Clone
CAT#: MG203230
- TrueORF®
Elf5 (tGFP-tagged) - Mouse E74-like factor 5 (Elf5)
ORF Plasmid: DDK
"NM_010125" in other vectors (4)
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CNY 4,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | ESE-2; ESE-5; ESE-5. |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203230 representing NM_010125
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGACTCCGTAACCCATAGCACCTTCCTGCCCAACGCATCCTTCTGTGACCCCCTGATGCCTTGGA CCGATCTGTTCAGCAATGAAGAATACTACCCTGCCTTTGAGCATCAGACAGCCTGTGATTCCTACTGGAC ATCAGTGCACCCTGAATACTGGACCAAGCGCCACGTCTGGGAATGGCTCCAATTCTGCTGCGACCAGTAC AAGCTTGATGCCAACTGCATCTCCTTCTGTCACTTCAACATCAGCGGCCTGCAGCTCTGCAGCATGACGC AGGAGGAGTTCATTGAGGCAGCCGGCATCTGTGGGGAGTACCTGTACTTCATTCTCCAGAACATTCGCTC GCAAGGTTACTCCTTTTTCAATGATGCTGAAGAAACCAAGACTGGCATCAAAGACTATGCTGATTCCAGT TGCTTGAAAACAAGTGGCATCAAGAGTCAAGACTGTCACAGCCGAACAAGCCTCCAAAGTTCTCACCTGT GGGAATTTGTCAGAGACTTGCTGCTGTCCCCTGAAGAGAACTGTGGCATCCTGGAATGGGAAGACAGGGA GCAGGGCATTTTCCGAGTGGTTAAGTCAGAAGCCCTGGCAAAGATGTGGGGACAAAGGAAGAAGAATGAC AGGATGACGTACGAGAAGCTGAGCCGAGCCCTGAGATACTACTATAAAACGGGAATTCTGGAGCGGGTTG ACCGGAGGTTAGTGTACAAATTTGGAAAGAACGCGCACGGGTGGCAGGAAGAGAAACTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203230 representing NM_010125
Red=Cloning site Green=Tags(s) MLDSVTHSTFLPNASFCDPLMPWTDLFSNEEYYPAFEHQTACDSYWTSVHPEYWTKRHVWEWLQFCCDQY KLDANCISFCHFNISGLQLCSMTQEEFIEAAGICGEYLYFILQNIRSQGYSFFNDAEETKTGIKDYADSS CLKTSGIKSQDCHSRTSLQSSHLWEFVRDLLLSPEENCGILEWEDREQGIFRVVKSEALAKMWGQRKKND RMTYEKLSRALRYYYKTGILERVDRRLVYKFGKNAHGWQEEKL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010125 |
ORF Size | 759 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010125.1, NP_034255.1 |
RefSeq Size | 2216 bp |
RefSeq ORF | 762 bp |
Locus ID | 13711 |
UniProt ID | Q8VDK3 |
MW | 29.9 kDa |
Gene Summary | Transcriptionally activator that may play a role in regulating the later stages of keratinocytes terminal differentiation (By similarity). Binds to DNA sequences containing the consensus nucleotide core sequence GGA[AT]. Transcriptionally activates the TK promoter.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC200780 | Elf5 (untagged) - Mouse E74-like factor 5 (Elf5), transcript variant 1, (10ug) |
CNY 2,400.00 |
|
MR203230 | Elf5 (Myc-DDK-tagged) - Mouse E74-like factor 5 (Elf5), transcript variant 1 |
CNY 2,400.00 |
|
MR203230L3 | Lenti ORF clone of Elf5 (Myc-DDK-tagged) - Mouse E74-like factor 5 (Elf5), transcript variant 1 |
CNY 4,750.00 |
|
MR203230L4 | Lenti ORF clone of Elf5 (mGFP-tagged) - Mouse E74-like factor 5 (Elf5), transcript variant 1 |
CNY 4,800.00 |