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Wdr77 (NM_027432) Mouse Tagged ORF Clone
CAT＃: MG205136
- TrueORF®
Wdr77 (tGFP-tagged) - Mouse WD repeat domain 77 (Wdr77)
ORF Plasmid: DDK
"NM_027432" in other vectors (3)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2610003I18Rik; 2610312E17Rik; C79984; p44/MEP50 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG205136 representing NM_027432
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGGAAGGACACCCCTCCTCCGCTCGTGCCCCCGGCGGCCCGCGAGTGGAACCTGCCCCCCAATGCGC CCGCATGCATGGAACGTCAATTGGAGGCTGCACGGTACCGGTCTGATGGTTCCCTTCTGCTCGGGGTCTC CAGCCTGAGTGGTCGCTGCTGGGTAGGTTCTCTGTGGTTTTTCAAGGATCCTAGTGCGGCCCCCAACGAA GGTTTCTGCTCTGCTGGCGTCCAGACCGAGGCTGGAGTAGCTGACCTCACTTGGGTGGGGGACAAAGGTA TCCTAGTGGCTTCTGATTCAGGTGCTGTTGAATTGTGGGAGCTAGATGAGAACGAGACACTTATAGTCAG CAAGTTCTGCAAGTATGAGCATGATGACATTGTGTCTACTGTCACTGTCCTGAGCTCTGGCACACAAGCT GTCAGTGGTAGCAAAGACTGCTGCATCAAAATTTGGGACCTGGCTCAGCAGGTATCACTGAATTCATACC GAGCTCACGCTGGACAGGTTACCTGTGTTGCTGCCTCTCCCCACAAAGACTCTGTGTTTCTTTCATGTAG TGAGGACAGTAGAATTTTGCTCTGGGATACCCGCTGTCCCAAGCCGGCATCACAGATGGCCTGCAATGCC TCTGGCTACCTCCCTACCGCTTTGGCTTGGCATCCTCAGCAGAGTGAAGTCTTTGTTTTTGGTGACGAGA ATGGATCTGTCTCCCTTGTGGACACCAAGAATGCAAGCTGTACCCTCAGCTCAGCTGTGCACTCCCAGGG TGTCACTAGACTGGTATTCTCCCCACACAGTGTCCCCCTCCTGACTTCTCTCAGTGAAGACTGTTCACTT GCTGTGCTGGATTCAAGCCTTTCTGAGGTGTTTAGAAGTCGAGCCCACAGAGACTTTGTGAGAGATGCTA CGTGGTCTCCACTCAATCACTCCCTTCTTACCACAGTTGGCTGGGACCATCAGGTCATCCACCATGTTGT GCCCTTAGAGCCTCTCCCAAACCCTGGACCTGACAGTGTTGTGGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG205136 representing NM_027432
Red=Cloning site Green=Tags(s) MRKDTPPPLVPPAAREWNLPPNAPACMERQLEAARYRSDGSLLLGVSSLSGRCWVGSLWFFKDPSAAPNE GFCSAGVQTEAGVADLTWVGDKGILVASDSGAVELWELDENETLIVSKFCKYEHDDIVSTVTVLSSGTQA VSGSKDCCIKIWDLAQQVSLNSYRAHAGQVTCVAASPHKDSVFLSCSEDSRILLWDTRCPKPASQMACNA SGYLPTALAWHPQQSEVFVFGDENGSVSLVDTKNASCTLSSAVHSQGVTRLVFSPHSVPLLTSLSEDCSL AVLDSSLSEVFRSRAHRDFVRDATWSPLNHSLLTTVGWDHQVIHHVVPLEPLPNPGPDSVVE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027432 |
| ORF Size | 1026 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027432.3, NP_081708.1 |
| RefSeq Size | 3553 bp |
| RefSeq ORF | 1029 bp |
| Locus ID | 70465 |
| UniProt ID | Q99J09 |
| Gene Summary | Non-catalytic component of the methylosome complex, composed of PRMT5, WDR77 and CLNS1A, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The methylosome complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage (PubMed:19584108).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MR205136 | Wdr77 (Myc-DDK-tagged) - Mouse WD repeat domain 77 (Wdr77) |
CNY 3,656.00 |
|
| MR205136L3 | Lenti ORF clone of Wdr77 (Myc-DDK-tagged) - Mouse WD repeat domain 77 (Wdr77) |
CNY 5,230.00 |
|
| MR205136L4 | Lenti ORF clone of Wdr77 (mGFP-tagged) - Mouse WD repeat domain 77 (Wdr77) |
CNY 5,230.00 |
