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Hgs (NM_008244) Mouse Tagged ORF Clone
CAT＃: MG210580
- TrueORF®
Hgs (tGFP-tagged) - Mouse HGF-regulated tyrosine kinase substrate (Hgs)
"NM_008244" in other vectors (5)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Hgr; Hrs; tn; ZFYVE8 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210580 representing NM_008244
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGCGAGGCAGCGGCACCTTCGAGCGTCTCCTAGACAAAGCCACCAGCCAGCTTCTGTTGGAGTCAG ACTGGGAGTCCATTCTACAGATCTGCGACCTGATCCGTCAGGGGGACACACAAGCAAAATATGCTGTAAA CTCCATCAAGAAGAAGGTTAATGATAAGAACCCACACGTGGCTTTGTATGCTCTGGAGGTGATGGAGTCT GTGGTAAAGAACTGTGGCCAGACAGTCCATGATGAAGTGGCCAACAAACAGACCATGGAAGAACTGAAGG AGCTGCTGAAGAGGCAAGTGGAAGTTAATGTTCGGAACAAGATCTTGTACCTGATCCAGGCCTGGGCACA TGCCTTCCGGAACGAACCCAAGTACAAGGTGGTCCAGGACACATACCAGATCATGAAGGTAGAAGGACAT GTCTTCCCTGAATTTAAGGAAAGTGATGCCATGTTCGCTGCTGAAAGAGCCCCTGACTGGGTGGATGCTG AGGAATGCCATCGGTGCAGAGTACAGTTTGGGGTGGTGACCCGCAAGCATCACTGCCGAGCATGTGGGCA GATCTTCTGTGGCAAGTGCTCCTCCAAGTACTCCACCATCCCCAAGTTCGGCATTGAGAAGGAGGTGCGC GTGTGTGAGCCCTGCTATGAGCAGCTGAACAAGAAGGCAGAAGGGAAGGCTTCCTCTACCACTGAGCTGC CCCCAGAGTACCTGACCAGCCCCCTGTCACAGCAGTCTCAGCTGCCCCCAAAGCGGGATGAGACAGCCCT GCAGGAAGAGGAGGAGCTACAGCTGGCTCTGGCCCTATCACAGTCAGAGGCTGAGGAGAAAGAGAGGATG AGACAGAAAACAACATATACAGCACATCCAAAGGCAGAGCCCACGCCCTTGGCTTCCTCTGCGCCCCCAG CTGGCAGCCTGTATTCCTCGCCTGTGAACTCATCAGCACCTCTGGCTGAGGACATCGACCCTGAGCTTGC AAGATACCTCAACCGGAACTACTGGGAGAAGAAACAGGAGGAAGCACGGAAGAGCCCCACACCATCTGCA CCTGTGCCCCTGACAGAGCCTGCTGCCCAGCCTGGAGAAGGACATACAGCCCCCAACAGCATGGCAGAGG CTCCTCTTCCAGAGACAGACTCTCAGCCCATAACTCCCTGCAGCGGCCCCTTTAGTGAGTACCAGAATGG GGAGTCGGAGGAGAGCCATGAGCAGTTCCTCAAGGCCCTGCAGAATGCCGTGAGCACTTTTGTCAACCGC ATGAAGAGCAACCACATGCGGGGACGCAGCATCACCAACGACTCGGCTGTGCTGTCACTCTTCCAGTCCA TCAACACCATGCACCCGCAGCTGCTCGAGCTGCTCAACCAGCTGGATGAGCGCAGGCTGTACTACGAGGG CCTTCAGGACAAGCTGGCACAGATACGTGACGCCCGAGGGGCTCTGAGCGCCCTGCGTGAAGAACACAGG GAGAAGCTGCGCCGGGCAGCTGAGGAGGCTGAGCGTCAACGCCAGATCCAGCTGGCACAGAAACTGGAGA TCATGAGACAGAAGAAGCAGGAGTACCTGGAGGTGCAGAGACAGCTAGCTATCCAGCGCCTGCAGGAACA GGAGAAGGAACGGCAGATGCGTCTGGAGCAACAGAAGCAGACTGTCCAGATGCGCGCCCAGATGCCTGCC TTTCCCTTGCCTTATGCCCAGCTCCAGGCTATGCCCACGGCTGGGGGTGTACTCTACCAGCCCTCAGGCC CAACCAGCTTCCCTGCCACCTTCAGCCCAGCAGGCTCAGTAGAGGGCTCTCCGATGCATGGTGTGTATAT GAGCCAGCCAGCCCCAGCCACTGGCCCCTACCCCAGCATGCCTGGCACAACAGCAGATCCCAGCATGGTC AGCGCCTACATGTACCCAACAGGTGCCCCTGGGGCACAGGCAGCCCCTCAGGCCCAGGCCGGGCCCACCA CCAGTCCTGCCTACTCCTCCTACCAGCCCACACCAACCCCAGGCTACCAGAGCGTGGCTTCTCAGGCCCC ACAGAGCCTCCCAGCCATCTCACAGCCTCCACAGACCAGCAACATAGGCTACATGGGGAGCCAGCCAATG TCCATGGGCTACCAGCCGTACAATATGCAGAATCTCATGACCGCCCTTCCCGGGCAGGATGCGTCTCTGC CAGCCCAGCAGCCCTACATCCCAGGGCAGCAGCCCCTGTACCAGCAGATGGCCCCCAGCACCGGCCCTCC CCAGCAGCAACCCCCTGTGGCCCAGCCAGCGCCTACACAGGGACCGCCAGCACAGGGCAGTGAGGCCCAG CTCATCTCCTTCGAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210580 representing NM_008244
Red=Cloning site Green=Tags(s) MGRGSGTFERLLDKATSQLLLESDWESILQICDLIRQGDTQAKYAVNSIKKKVNDKNPHVALYALEVMES VVKNCGQTVHDEVANKQTMEELKELLKRQVEVNVRNKILYLIQAWAHAFRNEPKYKVVQDTYQIMKVEGH VFPEFKESDAMFAAERAPDWVDAEECHRCRVQFGVVTRKHHCRACGQIFCGKCSSKYSTIPKFGIEKEVR VCEPCYEQLNKKAEGKASSTTELPPEYLTSPLSQQSQLPPKRDETALQEEEELQLALALSQSEAEEKERM RQKTTYTAHPKAEPTPLASSAPPAGSLYSSPVNSSAPLAEDIDPELARYLNRNYWEKKQEEARKSPTPSA PVPLTEPAAQPGEGHTAPNSMAEAPLPETDSQPITPCSGPFSEYQNGESEESHEQFLKALQNAVSTFVNR MKSNHMRGRSITNDSAVLSLFQSINTMHPQLLELLNQLDERRLYYEGLQDKLAQIRDARGALSALREEHR EKLRRAAEEAERQRQIQLAQKLEIMRQKKQEYLEVQRQLAIQRLQEQEKERQMRLEQQKQTVQMRAQMPA FPLPYAQLQAMPTAGGVLYQPSGPTSFPATFSPAGSVEGSPMHGVYMSQPAPATGPYPSMPGTTADPSMV SAYMYPTGAPGAQAAPQAQAGPTTSPAYSSYQPTPTPGYQSVASQAPQSLPAISQPPQTSNIGYMGSQPM SMGYQPYNMQNLMTALPGQDASLPAQQPYIPGQQPLYQQMAPSTGPPQQQPPVAQPAPTQGPPAQGSEAQ LISFD TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008244 |
| ORF Size | 2902 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008244.2 |
| RefSeq Size | 2858 bp |
| RefSeq ORF | 2328 bp |
| Locus ID | 15239 |
| UniProt ID | Q99LI8 |
| Gene Summary | Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221480 | Hgs (untagged) - Mouse HGF-regulated tyrosine kinase substrate (Hgs), transcript variant 2, (10ug) |
CNY 9,980.00 |
|
| MG227581 | Hgs (tGFP-tagged) - Mouse HGF-regulated tyrosine kinase substrate (Hgs) transcript variant 2, (10ug) |
CNY 8,460.00 |
|
| MR227581 | Hgs (Myc-DDK-tagged) - Mouse HGF-regulated tyrosine kinase substrate (Hgs), transcript variant 2 |
CNY 7,700.00 |
|
| MR227581L3 | Lenti ORF clone of Hgs (Myc-DDK-tagged) - Mouse HGF-regulated tyrosine kinase substrate (Hgs), transcript variant 2 |
CNY 9,600.00 |
|
| MR227581L4 | Lenti ORF clone of Hgs (mGFP-tagged) - Mouse HGF-regulated tyrosine kinase substrate (Hgs), transcript variant 2 |
CNY 9,600.00 |
