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Wwp1 (BC026829) Mouse Tagged ORF Clone
CAT＃: MG210655
- TrueORF®
Wwp1 (tGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (cDNA clone MGC:28572 IMAGE:4209397)
ORF Plasmid: DDK
"BC026829" in other vectors (4)
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CNY 8,650.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AIP5, SDRP1, Tiul1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210655 representing BC026829
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACGAGCAGCTGATTGTAAATGTGACCCCACAGACCACCTTGGAGTTCCGAGTTTGGAGTCACCAC ACTTTAAAGGCAGATGCTCTATTAGGAAAAGCAACAGTGGACTTGAAACAAGTGCTGTTAACACACAACA GAAAATGTTGCCTGTTGAAGGTACTATTGGAATTGATAATCATGTATCAACCAATACTGTTGTTCCCAAC TCCTGCTGTTCACATGTAGTTAATGGAGAAAACACACCTTCATCCCCGTCTCAGGTTGCTGCCAGACCCA AAAATGCTCCAGCTCCAAAGCCAGTCACATCTGCGCCCACCAGTGACACGGTTAATGGAGAATCATCATC TGTCCTAGCTGATAATACTTCAACCATGGGTACTTTACTACCATCTGAAGATACCACCTCGACTTCCAAT TGCACTAGCACCACCACCCAAGAGCCTCCTGTACAAGAACCCCCAGCATCCTCAGAACACAGTGAATGTA TTCCCTCTGCCAGTGCGGAAGTGGGACCAGAAGCTCGGAGCCTCATAGACCCTGACTCTGATTCTAGAAA TAATTCTGTGTTTGACAAAGTAAGGCAGCCAGAAGGGTGTGTAGAACCTCTGCGGCCACAGTCTGGAAAC ACCAACACAGAAGCTTTGCCATCAGGGTGGGAACAGAGAAAAGATCCTCATGGTAGAACCTATTATGTGG ATCATAATACTCGAACTACTACATGGGAGAGACCACAGCCTTTGCCTCCAGGTTGGGAAAGAAGAGTTGA TGATCGTGGAAGAGTTTATTATGTGGATCATAACACCAGAACAACAACCTGGCAGCGGCCTACTATGGAA TCTGTTCGAAATTTTGAACAGTGGCAGTCTCAGCGGAATCAATTGCAGGGAGCTATGCAACAATTCAACC AACGATACCTCTATTCGGCTTCCATGTTAGCTGCAGAAAATGACCCCTATGGACCCTTACCACCGGGCTG GGAAAAAAGAGTGGATTCAACAGACAGAGTTTACTTTGTGAATCACAATACAAAAACAACCCAGTGGGAA GATCCAAGAACTCAAGGCTTACCAAATGAAGAACCCCTGCCCGAAGGCTGGGAAATCAGGTATACTCGGG AAGGTGTTAGGTACTTTGTTGATCATAACACGAGAACAACAACATTCAAAGACCCTCGCAATGGGAAGTC ATCAGTAACTAAAGGTGGTCCACAGATTGCTTATGAACGCAGCTTTAGGTGGAAGCTTGCTCACTTCCGT TACTTGTGCCAGTCTAATGCACTACCGAGTCATGTAAAGATCAATGTGTCCCGACAGACATTATTTGAAG ATTCCTTCCAACAGATTATGGCATTAAAACCATATGACTTGAGGAGGCGATTATATGTAATATTTAGAGG AGAAGAAGGTCTTGATTATGGTGGCCTTGCAAGAGAATGGTTTTTCTTGCTTTCCCATGAAGTTTTGAAC CCAATGTATTGCTTATTTGAGTATGCAGGCAAGAATAACTATTGTTTACAGATAAATCCAGCATCAACTA TAAATCCTGACCATCTTTCATACTTCTGTTTTATTGGTCGCTTTATTGCAATGGCACTGTTCCATGGAAA GTTTATTGATACTGGTTTCTCTTTACCATTCTACAAGCGTATGCTAAGTAAAAAACTTACTATTAAGGAT TTGGAATCTATTGATACTGAATTTTACAACTCTCTAATCTGGATAAGAGATAACAATATTGAAGAATGTG GCTTAGAAATGTACTTTTCTGTAGACATGGAGATTCTGGGAAAAGTTACTTCACATGATTTAAAGTTGGG AGGTTCCAATATCCTGGTGACGGAAGAAAACAAAGATGAATATATTGGTTTAATGACAGAATGGCGTTTT TCTCGAGGAGTACAAGAACAGACCAAAGCTTTCCTTGATGGGTTTAATGAAGTTGTTCCCCTTCAGTGGC TACAGTACTTTGATGAAAAAGAATTGGAGGTTATGCTGTGTGGCATGCAAGAGGTTGACTTGGCAGACTG GCAGAGAAATACTGTTTATCGACATTATACAAGAAACAGCAAGCAAATTATTTGGTTTTGGCAGTTTGTG AAAGAGACTGATAATGAAGTACGAATGCGACTCTTGCAGTTTGTGACTGGAACCTGTCGCTTGCCTCTAG GAGGATTTGCTGAGCTCATGGGAAGTAATGGGCCTCAAAAGTTCTGTATTGAAAAAGTTGGCAAAGACAC CTGGTTACCAAGAAGCCATACATGTTTTAATCGCTTGGATCTACCACCATATAAGAGTTATGAACAACTA AAGGAAAAACTTCTTTTTGCAATAGAAGAGACAGAGGGATTTGGACAAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210655 representing BC026829
Red=Cloning site Green=Tags(s) MGRAADCKCDPTDHLGVPSLESPHFKGRCSIRKSNSGLETSAVNTQQKMLPVEGTIGIDNHVSTNTVVPN SCCSHVVNGENTPSSPSQVAARPKNAPAPKPVTSAPTSDTVNGESSSVLADNTSTMGTLLPSEDTTSTSN CTSTTTQEPPVQEPPASSEHSECIPSASAEVGPEARSLIDPDSDSRNNSVFDKVRQPEGCVEPLRPQSGN TNTEALPSGWEQRKDPHGRTYYVDHNTRTTTWERPQPLPPGWERRVDDRGRVYYVDHNTRTTTWQRPTME SVRNFEQWQSQRNQLQGAMQQFNQRYLYSASMLAAENDPYGPLPPGWEKRVDSTDRVYFVNHNTKTTQWE DPRTQGLPNEEPLPEGWEIRYTREGVRYFVDHNTRTTTFKDPRNGKSSVTKGGPQIAYERSFRWKLAHFR YLCQSNALPSHVKINVSRQTLFEDSFQQIMALKPYDLRRRLYVIFRGEEGLDYGGLAREWFFLLSHEVLN PMYCLFEYAGKNNYCLQINPASTINPDHLSYFCFIGRFIAMALFHGKFIDTGFSLPFYKRMLSKKLTIKD LESIDTEFYNSLIWIRDNNIEECGLEMYFSVDMEILGKVTSHDLKLGGSNILVTEENKDEYIGLMTEWRF SRGVQEQTKAFLDGFNEVVPLQWLQYFDEKELEVMLCGMQEVDLADWQRNTVYRHYTRNSKQIIWFWQFV KETDNEVRMRLLQFVTGTCRLPLGGFAELMGSNGPQKFCIEKVGKDTWLPRSHTCFNRLDLPPYKSYEQL KEKLLFAIEETEGFGQE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC026829 |
| ORF Size | 2361 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC026829.1 |
| RefSeq Size | 3107 bp |
| RefSeq ORF | 2363 bp |
| Locus ID | 107568 |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF (By similarity). Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220875 | Wwp1 (untagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (cDNA clone MGC:28572 IMAGE:4209397), (10ug) |
CNY 9,120.00 |
|
| MR210655 | Wwp1 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (cDNA clone MGC:28572 IMAGE:4209397) |
CNY 7,890.00 |
|
| MR210655L3 | Lenti ORF clone of Wwp1 (Myc-DDK-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (cDNA clone MGC:28572 IMAGE:4209397) |
CNY 9,790.00 |
|
| MR210655L4 | Lenti ORF clone of Wwp1 (mGFP-tagged) - Mouse WW domain containing E3 ubiquitin protein ligase 1 (cDNA clone MGC:28572 IMAGE:4209397) |
CNY 9,790.00 |
