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Afg3l2 (NM_027130) Mouse Tagged ORF Clone
CAT＃: MG210733
- TrueORF®
Afg3l2 (tGFP-tagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2)
ORF Plasmid: DDK
"NM_027130" in other vectors (4)
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CNY 7,700.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 2310036I02Rik; AW260507; Emv66; par |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG210733 representing NM_027130
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCACCGCTGCCTGCTGCTGTGGAGCCGGGGCGGCTGCCGTCGCGGCCTTCCTCCCCTGCTCGTGC CCAGAGGTTGCCTGGGTCCGGACCGGCGGCCCTGCCTCCGTACGCTCTATCAATATGCTACTGTCCAGAC AGCAAGCAGCAGGCGTTCTCTGCTGAGGGATGTAATTGCTGCTTATCAAAGATTCTGTTCTCGACCTCCC AAAGGATTTGAAAAGTACTTTCCTAATGGGAAAAACGGAAAAAAGGCCAGTGAGCCTAAGGAGGCTGTTG GAGAAAAAAAAGAACCACAGCCCTCGGGCCCCCAGCCTTCTGGAGGTGCAGGTGGTGGGGGAGGGAAGCG CCGTGGCAAGAAAGAAGATTCTCACTGGTGGTCCAGGTTCCAGAAGGGTGACTTCCCATGGGATGACAAG GATTTCAGGATGTACTTTCTCTGGACTGCTCTTTTTTGGGGTGGAGTCATGATTTACTTCGTGTTCAAGA GCTCTGGGAGAGAAATCACGTGGAAAGACTTTGTCAATAACTATCTTTCTAAGGGCGTGGTGGACAGACT AGAAGTTGTCAACAAGCGTTTTGTTCGTGTGACCTTTACACCAGGAAAAACTCCGGTTGATGGGCAATAC GTCTGGTTTAATATTGGCAGTGTTGACACATTTGAGCGGAATCTGGAGACTTTGCAGCAAGAATTGGGCA TAGAAGGGGAGAACCGGGTCCCTGTGGTTTATATTGCTGAGAGCGATGGCTCCTTCCTGCTGAGCATGTT GCCCACCGTACTCATTATCGCTTTTCTACTCTACACCATAAGAAGAGGGCCCGCTGGCATTGGTCGGACC GGCCGGGGAATGGGTGGACTCTTCAGCGTTGGGGAAACCACAGCCAAGGTCTTAAAGGATGAGATAGATG TGAAGTTTAAAGATGTGGCTGGCTGTGAGGAGGCCAAGCTAGAAATAATGGAATTCGTGAATTTCTTGAA AAACCCAAAGCAATATCAAGACCTAGGAGCAAAAATCCCAAAGGGTGCCATTCTCACCGGTCCCCCAGGT ACTGGGAAGACGCTGCTAGCTAAGGCCACAGCTGGAGAAGCCAATGTCCCCTTTATCACTGTGAGCGGAT CTGAGTTTCTGGAGATGTTTGTTGGCGTTGGTCCAGCCAGAGTCCGAGACTTATTTGCCCTTGCTCGGAA GAATGCGCCTTGCATTCTCTTCATTGATGAGATTGATGCTGTGGGAAGGAAGCGCGGCAGAGGCAACTTC GGTGGGCAGAGCGAGCAGGAGAACACACTCAACCAGCTGCTTGTGGAGATGGACGGCTTCAACACAACCA CCAATGTGGTCATCCTGGCAGGCACAAATCGACCAGACATCCTGGATCCAGCTCTGTTGAGACCAGGCCG CTTTGACAGGCAGATTTTTATTGGACCCCCAGACATAAAAGGACGAGCCTCAATCTTCAAAGTTCACCTT CGACCATTGAAGCTGGACAGTGCCTTGGAAAAAGATAAATTGGCCAGAAAACTGGCGTCCTTAACTCCAG GGTTTTCAGGCGCTGATGTTGCCAATGTCTGCAATGAAGCTGCTTTGATTGCTGCAAGACACCTTTCAGA TGCCATTAATGAGAAGCACTTCGAACAAGCGATTGAGCGAGTGATTGGAGGCTTGGAGAAAAAAACCCAA GTTCTGCAGCCTGAGGAGAAGAAGACGGTGGCTTACCACGAAGCAGGCCATGCGGTCGCTGGCTGGTATC TGGAGCATGCAGACCCACTCTTAAAGGTCTCCATCATCCCGCGTGGCAAGGGGCTGGGCTATGCTCAGTA CTTGCCCAAGGAGCAGTATCTGTACACAAAGGAGCAGCTGCTGGACAGGATGTGCATGACTCTGGGCGGC CGTGTCTCCGAGGAGATCTTCTTTGGGAGAATTACAACCGGTGCCCAGGACGACTTGAGGAAGGTTACCC AGAGTGCCTATGCCCAGATCGTTCAGTTTGGCATGAACGAGAAAGTGGGGCAGATCTCCTTTGACCTCCC ACGACAGGGGGACATGGTGTTAGAGAAGCCTTACAGTGAGGCCACTGCGAGGATGATAGACGATGAAGTG AGGATACTCATCAGCGATGCCTACAGAAGGACGGTGGCTCTTCTCACAGAGAAGAAGGCTGACGTGGAGA AGGTCGCTCTCTTACTGTTAGAAAAGGAAGTCCTAGACAAGAATGACATGGTCCAGCTTCTCGGTCCCAG ACCATTTACAGAAAAGTCCACATATGAAGAATTTGTGGAAGGCACTGGCAGCTTAGACGAGGACACTTCT CTTCCTGAAGGCCTTCAGGATTGGAACAAGGAGCGGGAGAAGGAGGAGAAGAAGGAGAAGGAGAAGGAGG AGCCGCTGAATGAGAAGGTTGTCAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG210733 representing NM_027130
Red=Cloning site Green=Tags(s) MAHRCLLLWSRGGCRRGLPPLLVPRGCLGPDRRPCLRTLYQYATVQTASSRRSLLRDVIAAYQRFCSRPP KGFEKYFPNGKNGKKASEPKEAVGEKKEPQPSGPQPSGGAGGGGGKRRGKKEDSHWWSRFQKGDFPWDDK DFRMYFLWTALFWGGVMIYFVFKSSGREITWKDFVNNYLSKGVVDRLEVVNKRFVRVTFTPGKTPVDGQY VWFNIGSVDTFERNLETLQQELGIEGENRVPVVYIAESDGSFLLSMLPTVLIIAFLLYTIRRGPAGIGRT GRGMGGLFSVGETTAKVLKDEIDVKFKDVAGCEEAKLEIMEFVNFLKNPKQYQDLGAKIPKGAILTGPPG TGKTLLAKATAGEANVPFITVSGSEFLEMFVGVGPARVRDLFALARKNAPCILFIDEIDAVGRKRGRGNF GGQSEQENTLNQLLVEMDGFNTTTNVVILAGTNRPDILDPALLRPGRFDRQIFIGPPDIKGRASIFKVHL RPLKLDSALEKDKLARKLASLTPGFSGADVANVCNEAALIAARHLSDAINEKHFEQAIERVIGGLEKKTQ VLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGG RVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARMIDDEV RILISDAYRRTVALLTEKKADVEKVALLLLEKEVLDKNDMVQLLGPRPFTEKSTYEEFVEGTGSLDEDTS LPEGLQDWNKEREKEEKKEKEKEEPLNEKVVS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027130 |
| ORF Size | 2406 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027130.2 |
| RefSeq Size | 3086 bp |
| RefSeq ORF | 2409 bp |
| Locus ID | 69597 |
| UniProt ID | Q8JZQ2 |
| Gene Summary | ATP-dependent protease which is essential for axonal and neuron development (PubMed:18337413, PubMed:27642048). In neurons, mediates degradation of SMDT1/EMRE before its assembly with the uniporter complex, limiting the availability of SMDT1/EMRE for MCU assembly and promoting efficient assembly of gatekeeper subunits with MCU (By similarity). Required for the maturation of paraplegin (SPG7) after its cleavage by mitochondrial-processing peptidase (MPP), converting it into a proteolytically active mature form.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221711 | Afg3l2 (untagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2), (10ug) |
CNY 10,260.00 |
|
| MR210733 | Afg3l2 (Myc-DDK-tagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2) |
CNY 5,888.00 |
|
| MR210733L3 | Lenti ORF clone of Afg3l2 (Myc-DDK-tagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2) |
CNY 8,930.00 |
|
| MR210733L4 | Lenti ORF clone of Afg3l2 (mGFP-tagged) - Mouse AFG3(ATPase family gene 3)-like 2 (yeast) (Afg3l2) |
CNY 8,930.00 |
