Ulk2 (BC053029) Mouse Tagged ORF Clone

CAT#: MG211498

  • TrueORF®

Ulk2 (tGFP-tagged) - Mouse Unc-51 like kinase 2 (C. elegans) (cDNA clone MGC:62255 IMAGE:5709559)

ORF Plasmid: DDK tGFP



  "BC053029" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 11,400.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Unc51.2, mKIAA0623
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211498 representing BC053029
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGTGGTGGGCGACTTCGAGTACTGCAAGCGGGACCTCGTGGGACACGGGGCCTTCGCTGTGGTCT
TCCGGGGGCGGCACCGCCAGAAAACTGATTGGGAGGTGGCTATTAAAAGTATTAATAAAAAGAACTTGTC
AAAATCACAAATTCTGCTTGGAAAGGAAATAAAAATCTTAAAGGAGCTTCAGCATGAAAACATCGTAGCG
CTCTATGATGTTCAGGAATTGCCCAACTCTGTCTTTCTGGTGATGGAGTATTGCAATGGTGGAGACCTGG
CAGATTATTTGCAAGCTAAAGGAACTCTGAGTGAAGATACTATCAGAGTGTTTCTCCATCAGATTGCGGC
AGCCATGCGAATCCTGCACAGCAAAGGGATAATCCACAGGGATCTCAAACCACAGAATATCCTGTTGTCT
TATGCCAATCGAAGGAAGTCGAATGTCAGTGGTATTCGTATTAAAATAGCTGATTTTGGTTTCGCACGGT
ACCTACATAGTAACACAATGGCAGCGACACTGTGTGGATCCCCAATGTACATGGCTCCCGAGGTTATTAT
GTCTCAACATTATGATGCTAAGGCAGATTTATGGAGCATAGGAACAGTGATCTATCAATGCCTAGTTGGA
AAACCACCTTTTCAGGCTAATAGTCCTCAGGACCTAAGGATGTTTTATGAAAAAAACAGGAGCTTAATGC
CTAGTATTCCCAGAGAAACATCACCTTACTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAATCAAAA
GGATAGAATGGACTTTGAAGCATTTTTCAGCCATCCTTTCCTTGAGCAAGTTCCAGTTAAAAAATCTTGC
CCAGTCCCAGTGCCTGTGTATTCTGGCCCTGTCCCTGGAAGCTCCTGCAGCAGCTCACCATCTTGTCGCT
TTGCTTCTCCACCATCCCTTCCAGATATGCAGCATATTCAGGAAGAAAACTTATCCTCCCCACCGTTGGG
TCCTCCCAACTATCTACAGGTGTCCAAAGACTCTGCGAGTAATAGTAGCAAGAACTCTTCTTGTGACACG
GATGACTTTGTTTTGGTTCCACACAACATCTCGTCAGACCACTCATATGACATGCCAATGGGGACTACGG
CCAGACGTGCTTCAAATGAATTCTTTATGTGTGGAGGGCAGTGTCAACCTACTGTGTCACCTCACAGCGA
AACAGCCCCAATTCCAGTTCCTACTCAAGTAAGGAATTATCAGCGCATAGAACAGAATCTTATATCCACT
GCCAGCTCTGGCACAAACCCACATGGTTCTCCAAGATCTGCAGTAGTACGAAGGTCTAATACCAGCCCCA
TGGGCTTCCTCCGGGTTGGGTCCTGCTCCCCTGTACCAGGAGACACAGTGCAGACAGGAGGACGAAGACT
CTCTACTGGCTCTTCCAGGCCTTACTCACCATCCCCTTTGGTTGGTACCATTCCTGAACAGTTTAGTCAG
TGCTGCTGTGGACATCCTCAGGGCCATGAAGCCAGGAGTAGGCACTCCTCAGGTTCTCCAGTGCCACAGA
CCCAGGCACCACAGTCACTCTTACTGGGTGCTAGACTGCAGAGTGCACCCACCCTCACCGATATCTATCA
GAACAAGCAGAAGCTCAGAAAGCAGCACTCTGACCCTGTGTGTCCGTCCCATGCTGGAGCTGGGTATAGT
TACTCACCTCAGCCTAGTCGGCCTGGCAGCCTTGGGACCTCTCCCACCAAGCACACGGGGTCCTCTCCAC
GGAATTCTGACTGGTTCTTTAAAACTCCTTTACCAACAATCATTGGCTCTCCTACTAAGACTACAGCTCC
TTTCAAAATCCCTAAAACACAAGCATCTTCTAACCTGTTAGCCTTGGTTACTCGTCATGGGCCTGCTGAA
AGCCAGTCCAAAGATGGGAATGACCCTCGTGAGTGTTCCCACTGCCTCTCAGTACAAGGAAGCGAGAGGC
ATCGATCTGAGCAGCAGCAGAGCAAGGCAGTGTTTGGCAGATCTGTCAGTACTGGGAAGTTATCAGAACA
ACAAGTAAAGGCACCTTTAGGTGGACACCAGGGCAGCACGGATAGTTTAAACACAGAACGACCAATGGAT
GTAGCTCCTGCAGGAGCCTGTGGTGTTATGCTGGCATTGCCAGCAGGAACAGCAGCAAGCGCCAGAGCTG
TCCTCTTCACCGTGGGGTCTCCTCCACACAGTGCCACAGCCCCCACTTGTACTCATATGGTCCTTCGAAC
AAGAACCACCTCAGTGGGGTCCAGCAGCTCAGGAGGTTCCTTGTGTTCTGCAAGTGGCCGAGTATGTGTG
GGCTCCCCTCCTGGACCAGGGTTGGGCTCTTCCCCACCAGGAGCAGAGGGAGCTCCCAGCCTAAGATACG
TGCCTTATGGTGCTTCACCACCCAGCCTAGAGGGTCTCATCACCTTTGAAGCCCCTGAACTACCAGAGGA
GACACTGATGGAGCGAGAGCACACAGACACCTTACGCCATCTGAACATGATGTTAATGTTTACTGAGTGT
GTGCTGGACCTGACGGCAGTGAGGGGTGGGAACCCTGAGCTGTGCACATCTGCTGTGTCCTTGTACCAGA
TTCAGGAGAGTGTAGTTGTGGACCAGATCAGCCAGCTAAGCAAAGATTGGGGGCGGGTGGAGCAGCTGGT
GTTGTACATGAAGGCAGCACAGCTGCTGGCGGCTTCCCTGCATCTCGCCAAAGCTCAGGTCAAGTCTGGG
AAGCTGAGCCCATCCATGGCTGTGAAACAAGTTGTTAAAAATCTGAATGAAAGATACAAATTCTGCATCA
CCATGTGCAAGAAACTTACAGAAAAGCTGAATCGCTTCTTCTCCGATAAACAGAGATTTATTGATGAAAT
CAACAGTGTGACTGCAGAGAAACTCATCTATAATTGTGCTGTGGAAATGGTTCAATCTGCAGCCCTGGAT
GAGATGTTTCAGCAGACTGAAGACATCGTTTATCGCTACCACAAGGCAGCCCTTCTTTTGGAAGGCTTAA
GTAAGATCCTGCAGGACCCTACAGATGTTGAAAATGTGCATAAGTATAAATGTAGTATTGAAAGAAGATT
GTCAGCACTCTGCTGTAGCACTGCAACTGTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211498 representing BC053029
Red=Cloning site Green=Tags(s)

MEVVGDFEYCKRDLVGHGAFAVVFRGRHRQKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENIVA
LYDVQELPNSVFLVMEYCNGGDLADYLQAKGTLSEDTIRVFLHQIAAAMRILHSKGIIHRDLKPQNILLS
YANRRKSNVSGIRIKIADFGFARYLHSNTMAATLCGSPMYMAPEVIMSQHYDAKADLWSIGTVIYQCLVG
KPPFQANSPQDLRMFYEKNRSLMPSIPRETSPYLANLLLGLLQRNQKDRMDFEAFFSHPFLEQVPVKKSC
PVPVPVYSGPVPGSSCSSSPSCRFASPPSLPDMQHIQEENLSSPPLGPPNYLQVSKDSASNSSKNSSCDT
DDFVLVPHNISSDHSYDMPMGTTARRASNEFFMCGGQCQPTVSPHSETAPIPVPTQVRNYQRIEQNLIST
ASSGTNPHGSPRSAVVRRSNTSPMGFLRVGSCSPVPGDTVQTGGRRLSTGSSRPYSPSPLVGTIPEQFSQ
CCCGHPQGHEARSRHSSGSPVPQTQAPQSLLLGARLQSAPTLTDIYQNKQKLRKQHSDPVCPSHAGAGYS
YSPQPSRPGSLGTSPTKHTGSSPRNSDWFFKTPLPTIIGSPTKTTAPFKIPKTQASSNLLALVTRHGPAE
SQSKDGNDPRECSHCLSVQGSERHRSEQQQSKAVFGRSVSTGKLSEQQVKAPLGGHQGSTDSLNTERPMD
VAPAGACGVMLALPAGTAASARAVLFTVGSPPHSATAPTCTHMVLRTRTTSVGSSSSGGSLCSASGRVCV
GSPPGPGLGSSPPGAEGAPSLRYVPYGASPPSLEGLITFEAPELPEETLMEREHTDTLRHLNMMLMFTEC
VLDLTAVRGGNPELCTSAVSLYQIQESVVVDQISQLSKDWGRVEQLVLYMKAAQLLAASLHLAKAQVKSG
KLSPSMAVKQVVKNLNERYKFCITMCKKLTEKLNRFFSDKQRFIDEINSVTAEKLIYNCAVEMVQSAALD
EMFQQTEDIVYRYHKAALLLEGLSKILQDPTDVENVHKYKCSIERRLSALCCSTATV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC053029
ORF Size 3111 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC053029.1
RefSeq Size 4413 bp
RefSeq ORF 3113 bp
Locus ID 29869
Gene Summary Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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