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Atp8a2 (NM_015803) Mouse Tagged ORF Clone
CAT＃: MG224181
- TrueORF®
Atp8a2 (tGFP-tagged) - Mouse ATPase aminophospholipid transporter-like class I type 8A member 2 (Atp8a2), (10ug)
ORF Plasmid: DDK
"NM_015803" in other vectors (4)
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CNY 12,640.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | agil; AI415030; Atpc1b; wl |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG224181 representing NM_015803
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCCGGGCCACGTCTGTTGGAGACCAGCTAGAGGCACCGGCCCGCATAATTTACCTCAACCAATCTC ATCTCAACAAATTCTGCGACAACCGGATCAGTACGGCCAAGTACAGCGTGTTGACATTTCTACCTCGATT CCTGTATGAGCAGATTAGGAGAGCTGCTAACGCCTTCTTCCTCTTCATTGCCTTATTACAGCAAATCCCA GACGTCTCGCCAACAGGAAGGTACACCACCCTGGTGCCCTTGGTCATCATTCTAACGATTGCAGGCATCA AAGAGATCATAGAAGATTTTAAACGACATAAGGCAGACAATGCAGTTAACAAGAAGAAAACAATAGTGTT AAGAAATGGCATGTGGCACACTATCATGTGGAAAGAGGTGGCAGTGGGAGACATCGTGAAGGTCCTCAAT GGGCAGTATCTTCCAGCAGACATGGTCCTGTTCTCCTCCAGTGAGCCCCAGGGGATGTGCTATGTTGAAA CTGCTAACCTGGATGGCGAGACGAACCTTAAAATACGACAGGGCTTGAGCCACACAACCGACATGCAGAC GAGGGACGTGCTCATGAAGCTGTCGGGAAGGATAGAGTGTGAAGGGCCCAACCGCCACCTCTACGACTTC ACCGGCAACCTGCACTTGGATGGGAAGAGCTCCGTTGCCCTTGGACCTGACCAGATCCTTCTGAGAGGCA CCCAGCTCAGGAACACACAGTGGGTCTTCGGCGTCGTTGTGTACACTGGACATGACAGCAAGCTCATGCA GAATTCAACAAAAGCACCTCTCAAGAGATCAAATGTTGAGAAGGTAACCAACGTGCAGATCCTGGTGCTG TTTGGTATCCTCCTGGTCATGGCGCTGGTGAGCTCTGTGGGGGCCCTGTTCTGGAATGGGTCTCATGGCG GAAAGAGTTGGTACATCAAGAAGATGGACACGAACTCAGATAATTTTGGCTACAACTTGCTAACGTTTAT CATCTTGTACAACAATCTGATTCCTATCAGCCTGCTGGTGACACTTGAGGTTGTGAAATACACGCAAGCC CTCTTCATAAACTGGGACATGGATATGTATTATATTGAAAATGACACTCCTGCAATGGCCAGGACATCAA ACCTCAACGAAGAGCTGGGGCAGGTAAAATACCTGTTTTCAGACAAGACTGGAACTCTTACATGTAATAT CATGAACTTCAAGAAGTGTAGCATTGCTGGTGTGACCTATGGCCACTTTCCGGAACTAGCCAGAGAGCAG TCCTCAGATGACTTCTGCCGGATGACTTCTTGTACCAATGACTCATGTGACTTCAATGATCCCAGGCTGT TAAAGAACATCGAGGATCAGCACCCTACAGCCCCTTGCATACAAGAGTTCCTTACCCTGCTGGCTGTGTG TCACACCGTCGTCCCCGAGAAGGATGGAGATGAAATTATCTACCAAGCCTCGTCCCCAGATGAAGCTGCT TTGGTCAAAGGAGCTAAGAAGCTTGGCTTTGTGTTTACCGGGAGGACGCCGTACTCGGTCATCATCGAAG CGATGGGACAAGAACAGACATTCGGGATCCTCAATGTTCTAGAATTTTCTAGTGACAGGAAAAGAATGTC TGTCATTGTCCGACTGCCATCAGGACAACTTCGACTCTACTGCAAGGGAGCTGATAACGTCATCTTTGAA AGACTCTCAAAGGACTCAAAGTACATGGAGGAGACGCTATGCCATCTGGAATATTTTGCCACAGAAGGCC TGCGGACTCTGTGCGTGGCCTACGCAGATCTATCTGAGAATGAGTATGAGGAGTGGCTGAAAGTCTATCA AGAGGCCAGCATCATTCTGAAGGACAGAGCCCAGAGGCTGGAAGAGTGTTACGAGATCATTGAGAAGAAT TTACTGTTACTTGGAGCTACAGCCATCGAAGACCGTCTTCAAGCCGGCGTTCCAGAAACCATAGCCACTC TGCTGAAGGCAGAAATCAAAATCTGGGTGTTGACAGGAGACAAACAAGAAACTGCAATTAATATCGGGTA TTCCTGTAGGTTGGTGTCGCAGAACATGGCCCTTATCCTATTGAAGGAGGACTCTTTAGATGCAACAAGG GCGGCCATCACTCAGCACTGCACAGATCTTGGAAATTTGCTGGGCAAAGAGAACGATGTGGCCCTCATCA TCGATGGCCATACCCTGAAGTATGCACTCTCCTTTGAAGTCCGCAGAAGTTTCCTGGACCTGGCGCTCTC ATGCAAAGCTGTCATCTGCTGCAGAGTGTCCCCTCTGCAGAAGTCTGAGATCGTGGATGTTGTGAAGAAG CGAGTGAAAGCCATCACCCTGGCCATCGGAGACGGTGCCAATGACGTGGGGATGATCCAGACAGCCCACG TAGGGGTCGGGATCAGCGGGAACGAGGGAATGCAGGCTACCAACAACTCAGATTATGCCATCGCACAGTT TTCCTACCTGGAGAAGCTGCTTTTGGTCCACGGAGCCTGGAGCTACAACCGGGTGACCAAGTGTATCCTG TACTGTTTCTACAAGAATGTGGTCCTCTACATCATCGAGCTATGGTTCGCCTTTGTGAATGGATTTTCTG GGCAGATTTTATTCGAGCGCTGGTGCATCGGCTTGTACAATGTGATCTTCACGGCATTGCCGCCCTTCAC TCTGGGGATCTTCGAGAGGTCTTGTACTCAGGAGAGCATGCTCAGGTTCCCACAGCTTTACAGAATCACT CAGAACGCTGAAGGTTTCAACACTAAGGTTTTCTGGGGTCACTGCATCAATGCCTTGGTTCATTCCCTCA TCCTCTTCTGGGTTCCCATGAAAGCGCTGGAGCATGATACTCCAGTAACCAGCGGTCATGCCACAGACTA TTTGTTTGTTGGAAATATTGTTTACACGTACGTTGTGGTTACAGTTTGTTTGAAAGCTGGTTTGGAGACG ACAGCTTGGACGAAATTCAGTCACCTGGCGGTGTGGGGAAGCATGCTGATCTGGTTGGTGTTCTTTGGTG TCTATTCAACCATCTGGCCGACCATCCCCATTGCTCCTGACATGAAAGGGCAGGCAACTATGGTCCTGAG CTCTGCGTACTTCTGGTTGGGATTGTTCCTGGTTCCGACTGCGTGTTTGATTGAAGACGTGGCGTGGAGA GCGGCCAAGCACACCTGCAAAAAGACTCTGCTGGAGGAGGTGCAGGAGCTGGAGACCAAGTCCCGAGTGA TGGGCAAGGCGATGCTTCGAGACAGTAACGGAAAGAGGATGAACGAGCGTGACCGTCTGATCAAGAGGCT CAGCAGGAAGACACCTCCAACCCTCTTCCGAACAGGCTCCATCCAGCAGTGTGTCAGCCATGGGTATGCC TTTTCTCAAGAAGAGCATGGAGCTGTTACACAGGAGGAAATAGTCCGCGCTTATGATACCACCAAAGAGA ATTCAAGGAAGAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG224181 representing NM_015803
Red=Cloning site Green=Tags(s) MSRATSVGDQLEAPARIIYLNQSHLNKFCDNRISTAKYSVLTFLPRFLYEQIRRAANAFFLFIALLQQIP DVSPTGRYTTLVPLVIILTIAGIKEIIEDFKRHKADNAVNKKKTIVLRNGMWHTIMWKEVAVGDIVKVLN GQYLPADMVLFSSSEPQGMCYVETANLDGETNLKIRQGLSHTTDMQTRDVLMKLSGRIECEGPNRHLYDF TGNLHLDGKSSVALGPDQILLRGTQLRNTQWVFGVVVYTGHDSKLMQNSTKAPLKRSNVEKVTNVQILVL FGILLVMALVSSVGALFWNGSHGGKSWYIKKMDTNSDNFGYNLLTFIILYNNLIPISLLVTLEVVKYTQA LFINWDMDMYYIENDTPAMARTSNLNEELGQVKYLFSDKTGTLTCNIMNFKKCSIAGVTYGHFPELAREQ SSDDFCRMTSCTNDSCDFNDPRLLKNIEDQHPTAPCIQEFLTLLAVCHTVVPEKDGDEIIYQASSPDEAA LVKGAKKLGFVFTGRTPYSVIIEAMGQEQTFGILNVLEFSSDRKRMSVIVRLPSGQLRLYCKGADNVIFE RLSKDSKYMEETLCHLEYFATEGLRTLCVAYADLSENEYEEWLKVYQEASIILKDRAQRLEECYEIIEKN LLLLGATAIEDRLQAGVPETIATLLKAEIKIWVLTGDKQETAINIGYSCRLVSQNMALILLKEDSLDATR AAITQHCTDLGNLLGKENDVALIIDGHTLKYALSFEVRRSFLDLALSCKAVICCRVSPLQKSEIVDVVKK RVKAITLAIGDGANDVGMIQTAHVGVGISGNEGMQATNNSDYAIAQFSYLEKLLLVHGAWSYNRVTKCIL YCFYKNVVLYIIELWFAFVNGFSGQILFERWCIGLYNVIFTALPPFTLGIFERSCTQESMLRFPQLYRIT QNAEGFNTKVFWGHCINALVHSLILFWVPMKALEHDTPVTSGHATDYLFVGNIVYTYVVVTVCLKAGLET TAWTKFSHLAVWGSMLIWLVFFGVYSTIWPTIPIAPDMKGQATMVLSSAYFWLGLFLVPTACLIEDVAWR AAKHTCKKTLLEEVQELETKSRVMGKAMLRDSNGKRMNERDRLIKRLSRKTPPTLFRTGSIQQCVSHGYA FSQEEHGAVTQEEIVRAYDTTKENSRKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015803 |
| ORF Size | 3444 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_015803.2, NP_056618.1 |
| RefSeq Size | 3685 bp |
| RefSeq ORF | 3447 bp |
| Locus ID | 50769 |
| UniProt ID | P98200 |
| Gene Summary | Catalytic component of a P4-ATPase flippase complex which catalyzes the hydrolysis of ATP coupled to the transport of aminophospholipids from the outer to the inner leaflet of various membranes and ensures the maintenance of asymmetric distribution of phospholipids. Phospholipid translocation seems also to be implicated in vesicle formation and in uptake of lipid signaling molecules. Reconstituted to liposomes, the ATP8A2:TMEM30A flippase complex predomiminantly transports phosphatidylserine (PS) and to a lesser extent phosphatidylethanolamine (PE). ATP8A2:TMEM30A may be involved in regulation of neurite outgrowth. Proposed to function in the generation and maintenance of phospholipid asymmetry in photoreceptor disk membranes and neuronal axon membranes. May be involved in vesicle trafficking in neuronal cells. Required for normal visual and auditory function; involved in photoreceptor and inner ear spiral ganglion cell survival.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223737 | Atp8a2 (untagged) - Mouse ATPase, aminophospholipid transporter-like, class I, type 8A, member 2 (Atp8a2), (10ug) |
CNY 14,820.00 |
|
| MR224181 | Atp8a2 (Myc-DDK-tagged) - Mouse ATPase, aminophospholipid transporter-like, class I, type 8A, member 2 (Atp8a2) |
CNY 11,992.00 |
|
| MR224181L3 | Lenti ORF clone of Atp8a2 (Myc-DDK-tagged) - Mouse ATPase, aminophospholipid transporter-like, class I, type 8A, member 2 (Atp8a2) |
CNY 13,400.00 |
|
| MR224181L4 | Lenti ORF clone of Atp8a2 (mGFP-tagged) - Mouse ATPase, aminophospholipid transporter-like, class I, type 8A, member 2 (Atp8a2) |
CNY 13,400.00 |
