Tbcb (NM_025548) Mouse Tagged ORF Clone
CAT#: MR203058
- TrueORF®
Tbcb (Myc-DDK-tagged) - Mouse tubulin folding cofactor B (Tbcb)
ORF Plasmid: tGFP
"NM_025548" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2410007D12Rik; AU041393; CG22; Ckap1; CKAPI |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR203058 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGACGGGGATATCGGCGCCCACCGTGATGGTTTTCATCAGCAGCTCCCTCAACAGCTTCCGTT CGGAGAAGCGATACAGTCGTAGCCTCACCATCGCAGAGTTCAAGTGTAAACTGGAGCTGGTGGTGGGCAG CCCTGCTTCCTGCATGGAACTGGAGCTATATGGAGCAGACGACAAGTTCTACAGCAAGTTGGATCAGGAG GATGCACTGCTGGGCTCCTATCCTGTGGATGACGGCTGTCGCATCCATGTCATTGACCACAGTGGCGTCC GGCTCGGAGAGTATGAGGATGTGTCCAAAGTGGAGAAATACGAGATCTCACCAGAAGCTTACGAACGGAG GCAGAACACAGTTCGATCCTTCATGAAACGCAGCAAGCTAGGCCCGTACAATGAAGAACTCCGGGCCCAG CAAGAGGCAGAGGCTGCCCAGCGTCTCAGTGAGGAGAAGGCCCAAGCCAGTGCCATCTCTGTGGGGAGCC GTTGTGAGGTGCGGGCACCCGACCACTCCCTTCGCAGGGGCACTGTCATGTATGTAGGACTCACAGATTT CAAGCCAGGCTACTGGGTTGGTGTCCGATATGATGAGCCGTTAGGAAAAAACGATGGCAGTGTGAATGGG AAACGCTACTTTGAATGCCAGGCCAAGTATGGTGCCTTTGTAAAGCCATCAGCTGTCACCGTAGGAGATT TCCCAGAGGAGGACTACGGGTTGGACGAGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR203058 protein sequence
Red=Cloning site Green=Tags(s) MEVTGISAPTVMVFISSSLNSFRSEKRYSRSLTIAEFKCKLELVVGSPASCMELELYGADDKFYSKLDQE DALLGSYPVDDGCRIHVIDHSGVRLGEYEDVSKVEKYEISPEAYERRQNTVRSFMKRSKLGPYNEELRAQ QEAEAAQRLSEEKAQASAISVGSRCEVRAPDHSLRRGTVMYVGLTDFKPGYWVGVRYDEPLGKNDGSVNG KRYFECQAKYGAFVKPSAVTVGDFPEEDYGLDEM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_025548 |
ORF Size | 735 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_025548.4 |
RefSeq Size | 1173 bp |
RefSeq ORF | 735 bp |
Locus ID | 66411 |
UniProt ID | Q9D1E6 |
MW | 27.4 kDa |
Gene Summary | Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (By similarity). Involved in regulation of tubulin heterodimer dissociation (PubMed:17184771). May function as a negative regulator of axonal growth (PubMed:17217416).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207582 | Tbcb (untagged) - Mouse tubulin folding cofactor B (Tbcb), (10ug) |
CNY 3,990.00 |
|
MG203058 | Tbcb (tGFP-tagged) - Mouse tubulin folding cofactor B (Tbcb) |
CNY 2,850.00 |
|
MR203058L3 | Lenti ORF clone of Tbcb (Myc-DDK-tagged) - Mouse tubulin folding cofactor B (Tbcb) |
CNY 4,750.00 |
|
MR203058L4 | Lenti ORF clone of Tbcb (mGFP-tagged) - Mouse tubulin folding cofactor B (Tbcb) |
CNY 4,750.00 |