United States
Germany
Japan
United Kingdom
China
Sec61a1 (NM_016906) Mouse Tagged ORF Clone
CAT＃: MR207628
- TrueORF®
Sec61a1 (Myc-DDK-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1)
ORF Plasmid: tGFP
"NM_016906" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA408394; AA410007; Sec61a |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR207628 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATCAAATTTCTGGAAGTCATCAAGCCATTCTGTGTCATCTTGCCGGAAATTCAGAAGCCAGAGA GAAAGATTCAGTTTAAGGAGAAAGTTCTGTGGACAGCCATCACCCTCTTCATCTTCTTAGTATGCTGTCA GATCCCCCTGTTTGGCATCATGTCTTCAGACTCAGCTGACCCGTTCTACTGGATGCGAGTGATTCTGGCC TCTAACAGAGGTACCTTAATGGAGCTGGGCATCTCTCCTATTGTCACCTCCGGTCTTATCATGCAGCTCT TAGCTGGTGCCAAGATAATTGAAGTTGGGGACACCCCGAAAGACCGAGCTCTCTTCAATGGAGCCCAGAA GTTGTTTGGAATGATCATTACTATCGGTCAGTCCATTGTGTATGTGATGACGGGCATGTACGGGGACCCT TCGGAGATGGGTGCTGGAATCTGCCTGCTGATCACCATTCAGCTCTTTGTTGCTGGCTTAATTGTCCTAC TTTTGGATGAACTTCTGCAAAAAGGGTACGGCTTGGGCTCTGGCATTTCTCTCTTCATTGCGACTAACAT CTGTGAGACCATTGTGTGGAAGGCATTCAGCCCCACCACTGTCAACACTGGCCGAGGGATGGAATTCGAA GGCGCCATCATAGCGCTATTCCACCTGCTGGCCACCCGCACAGACAAGGTCCGAGCCCTTCGAGAGGCAT TCTACCGCCAGAACCTCCCCAACCTCATGAACCTGATTGCCACCATCTTCGTCTTTGCCGTTGTCATCTA TTTCCAGGGCTTCCGAGTGGACCTTCCAATCAAGTCGGCGCGTTACCGAGGCCAGTACAACACCTATCCC ATCAAGCTCTTCTACACCTCCAACATCCCCATCATCTTGCAGTCTGCTCTGGTGTCCAACCTGTATGTCA TCTCCCAGATGCTGTCAGCACGCTTCAGTGGCAACCTGCTGGTTAGCCTGCTGGGCACCTGGTCGGATAC ATCATCTGGGGGCCCAGCCCGAGCTTACCCCGTTGGTGGCCTGTGTTACTACCTGTCTCCTCCGGAATCG TTCGGCTCAGTGCTGGAGGACCCTGTCCATGCGGTCGTGTACATCGTGTTCATGCTGGGCTCCTGTGCAT TCTTCTCCAAAACATGGATCGAAGTCTCAGGTTCTTCTGCCAAAGATGTTGCAAAGCAGCTGAAGGAGCA GCAGATGGTGATGAGGGGCCACAGGGAGACCTCCATGGTGCATGAGCTCAACCGGTACATCCCCACAGCC GCAGCCTTTGGTGGGCTGTGCATTGGGGCCCTCTCTGTCCTGGCTGACTTCCTGGGTGCTATCGGGTCTG GAACTGGGATCCTGCTTGCGGTCACCATCATCTACCAGTACTTTGAGATCTTCGTCAAGGAACAGAGCGA GGTGGGCAGCATGGGAGCCCTTCTCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR207628 protein sequence
Red=Cloning site Green=Tags(s) MAIKFLEVIKPFCVILPEIQKPERKIQFKEKVLWTAITLFIFLVCCQIPLFGIMSSDSADPFYWMRVILA SNRGTLMELGISPIVTSGLIMQLLAGAKIIEVGDTPKDRALFNGAQKLFGMIITIGQSIVYVMTGMYGDP SEMGAGICLLITIQLFVAGLIVLLLDELLQKGYGLGSGISLFIATNICETIVWKAFSPTTVNTGRGMEFE GAIIALFHLLATRTDKVRALREAFYRQNLPNLMNLIATIFVFAVVIYFQGFRVDLPIKSARYRGQYNTYP IKLFYTSNIPIILQSALVSNLYVISQMLSARFSGNLLVSLLGTWSDTSSGGPARAYPVGGLCYYLSPPES FGSVLEDPVHAVVYIVFMLGSCAFFSKTWIEVSGSSAKDVAKQLKEQQMVMRGHRETSMVHELNRYIPTA AAFGGLCIGALSVLADFLGAIGSGTGILLAVTIIYQYFEIFVKEQSEVGSMGALLF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_016906 |
| ORF Size | 1431 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_016906.4, NP_058602.1 |
| RefSeq Size | 3039 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 53421 |
| UniProt ID | P61620 |
| MW | 52.3 kDa |
| Gene Summary | Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins. Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis (By similarity). Plays a critical role in nephrogenesis, specifically at pronephros stage (PubMed:27392076).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216417 | Sec61a1 (untagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1), (10ug) |
CNY 6,180.00 |
|
| MG207628 | Sec61a1 (tGFP-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) |
CNY 3,710.00 |
|
| MR207628L3 | Lenti ORF clone of Sec61a1 (Myc-DDK-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) |
CNY 5,230.00 |
|
| MR207628L4 | Lenti ORF clone of Sec61a1 (mGFP-tagged) - Mouse Sec61 alpha 1 subunit (S. cerevisiae) (Sec61a1) |
CNY 5,230.00 |
其它Sec61a1产品
