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Cyp46a1 (NM_010010) Mouse Tagged ORF Clone
CAT＃: MR208027
- TrueORF®
Cyp46a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1)
ORF Plasmid: tGFP
"NM_010010" in other vectors (4)
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CNY 5,040.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cyp46 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR208027 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCCCGGGCTGCTGCTGCTCGGCAGCGCCGTCCTGCTCGCCTTCGGCCTCTGCTGCACCTTCGTGC ATCGCGCTCGCAGCCGCTATGAGCACATCCCCGGGCCGCCGCGGCCCAGCTTCCTTCTTGGACATCTCCC CTACTTTTGGAAAAAGGACGAAGATTGTGGCCGTGTGCTCCAAGATGTGTTTCTGGATTGGGCTAAGAAG TATGGTCCTGTTGTAAGAGTCAATGTCTTCTACAAGACCTCAGTCATTGTCACGAGTCCGGAGTCAGTCA AGAAGTTCCTGATGTCCACCAAGTACAACAAGGACTCCAAGATGTACCGCGCGCTTCAGACTGTGTTTGG GGAGAGACTGTTTGGCCAGGGCTTGGTGTCTGAATGTGACTATGGGCGCTGGTACAAGCAGAGGAAAGTC ATGGACTTGGCCTTCAGCCGCAGCTCCTTGGTCAGCCTGATGGAGACGTTCAACGAGAAGGCGGAGCAGC TGGTGGAAATCCTAGAAGCTAAGGCAGATGGACAGACACCCGTGTCCATGCAGGACATGCTGACCTGTGC CACCATCGACATCCTGGCCAAGGCAGCTTTTGGGATGGAGACCAGTATGTTACTGGGTGCCCAGAAACCG CTGTCCCAGGCAGTGAAGGTCATGCTGGAGGGCATCAGTGCATCCCGTAACACCCTGGCAAAGTTCATGC CAGGGAAGAGAAAGCAGCTTCGGGAGATCCGCGAGAGCATCCGTCTGCTGCGCCAGGTGGGGAAGGATTG GGTGCAGCGCCGCCGCGAAGCCCTGAAGAGGGGCGAGGACATGCCGGCTGACATCCTCACGCAGATCCTC AAAGCTGAAGAGGGAGCTCAGGACGATGAGGTTCTGCTGGACAACTTTGTCACCTTCTTCATTGCGGGTC ATGAGACTTCTGCCAACCATCTGGCATTCACAGTGATGGAGTTGTCTCGCCAGCCAGAGATCGTGGCAAG GCTGCAGGCCGAGGTGGATGAGGTTGTCGGTTCCAAGAGGCACCTGGACTACGAGGATCTGGGGAGACTG CAGTATCTGTCGCAGGTCCTCAAAGAGTCTCTGAGGCTGTACCCGCCAGCGTGGGGCACCTTTCGCCTGC TGGAGGAGGAGACCTTGATTGATGGGGTCAGAGTCCCTGGCAATACTCCTCTCCTGTTCAGCACCTACGT CATGGGGAGAATGGACACCTACTTTGAAGACCCATTGACTTTCAACCCTGACCGCTTCGGCCCTGGAGCG CCCAAGCCACGGTTCACTTACTTTCCCTTTTCCCTGGGCCACCGGTCCTGCATCGGCCAACAGTTTGCTC AGATGGAGGTGAAAGTTGTCATGGCCAAGCTGCTTCAGAGGATCGAGTTCCGGCTAGTGCCTGGGCAGCG CTTTGGGCTGCAAGAGCAGGCTACGCTCAAGCCATTGGACCCCGTGCTGTGCACCCTGAGGCCCCGGGGC TGGCAGCCTGCACCCCCACCCCCACCCTGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR208027 protein sequence
Red=Cloning site Green=Tags(s) MSPGLLLLGSAVLLAFGLCCTFVHRARSRYEHIPGPPRPSFLLGHLPYFWKKDEDCGRVLQDVFLDWAKK YGPVVRVNVFYKTSVIVTSPESVKKFLMSTKYNKDSKMYRALQTVFGERLFGQGLVSECDYGRWYKQRKV MDLAFSRSSLVSLMETFNEKAEQLVEILEAKADGQTPVSMQDMLTCATIDILAKAAFGMETSMLLGAQKP LSQAVKVMLEGISASRNTLAKFMPGKRKQLREIRESIRLLRQVGKDWVQRRREALKRGEDMPADILTQIL KAEEGAQDDEVLLDNFVTFFIAGHETSANHLAFTVMELSRQPEIVARLQAEVDEVVGSKRHLDYEDLGRL QYLSQVLKESLRLYPPAWGTFRLLEEETLIDGVRVPGNTPLLFSTYVMGRMDTYFEDPLTFNPDRFGPGA PKPRFTYFPFSLGHRSCIGQQFAQMEVKVVMAKLLQRIEFRLVPGQRFGLQEQATLKPLDPVLCTLRPRG WQPAPPPPPC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010010 |
| ORF Size | 1503 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010010.1, NP_034140.1 |
| RefSeq Size | 2146 bp |
| RefSeq ORF | 1503 bp |
| Locus ID | 13116 |
| UniProt ID | Q9WVK8 |
| MW | 56.8 kDa |
| Gene Summary | P450 monooxygenase that plays a major role in cholesterol homeostasis in the brain. Primarily catalyzes the hydroxylation (with S stereochemistry) at C-24 of cholesterol side chain, triggering cholesterol diffusion out of neurons and its further degradation (PubMed:10377398, PubMed:16505352, PubMed:28190002). By promoting constant cholesterol elimination in neurons, may activate the mevalonate pathway and coordinate the synthesis of new cholesterol and nonsterol isoprenoids involved in synaptic activity and learning (PubMed:16505352). Further hydroxylates cholesterol derivatives and hormone steroids on both the ring and side chain of these molecules, converting them into active oxysterols involved in lipid signaling and biosynthesis (By similarity). Acts as an epoxidase converting cholesta-5,24-dien-3beta-ol/desmosterol into (24S),25-epoxycholesterol, an abundant lipid ligand of nuclear NR1H2 and NR1H3 receptors shown to promote neurogenesis in developing brain (By similarity). May also catalyze the oxidative metabolism of xenobiotics, such as clotrimazole (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC201022 | Cyp46a1 (untagged) - Mouse cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1), (10ug) |
CNY 5,592.00 |
|
| MG208027 | Cyp46a1 (tGFP-tagged) - Mouse cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1) |
CNY 7,192.00 |
|
| MR208027L3 | Lenti ORF clone of Cyp46a1 (Myc-DDK-tagged) - Mouse cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1) |
CNY 6,940.00 |
|
| MR208027L4 | Lenti ORF clone of Cyp46a1 (mGFP-tagged) - Mouse cytochrome P450, family 46, subfamily a, polypeptide 1 (Cyp46a1) |
CNY 6,940.00 |
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