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Cdk12 (NM_001109628) Mouse Tagged ORF Clone
CAT＃: MR215624
- TrueORF®
Cdk12 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 2
ORF Plasmid: tGFP
"NM_001109628" in other vectors (4)
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CNY 14,730.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810022J16Rik; AI646528; Crk7; Crkrs; D11Ertd752e; Pksc |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR215624 representing NM_001109628
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGCCCAATTCCGAGAGACATGGGGGCAAGAAGGACGGGAGCGGAGGAGCTTCTGGAACTTCGCAGCCGT CATCGGGAGGTGGCAGCTCCAACAGCAGGGAGCGTCACCGCTTGGTGTCGAAGCACAAGCGGCATAAGTC CAAGCACTCCAAAGACGTGGGGCTGGTGACCCCCGAAGCGGCATCTTTGGGTACCATAATCAAACCACTG GTGGAGTACGATGACATCAGCTCTGATTCAGACACCTTCTCCGATGACACGGCCTTCAAATCAGACCGGA GGGAGAACGAGGAACGTCGGGGAACGGATCGGAGCGATCGCCTGCACCGACATCGTCACCACCAGCACCG GCGGTCCCGAGACTTGCTAAAAACTAAACAGACGGAAAAGGAAAAAAATCAGGAAGTCTCCAAATCTGGA TCTATGAAGGACCGGGTATCGGGCAGTTCCAAACGGTCCGTGGAGGGGAGTGATGATTATGGGAAGGCCC AGCTATCCAAAAGCGGCAGCAAGGAATCCAGGTCGTCCAAAATGCACAAGGAGAAGACCCGCAAAGAGCG AGAGTTAAAGTCTGGATACAAGGACCGGAGTAAAAGTCATCGGAAAAGGGAAACACCCAAAAGTTACAAA ACCGTGGCTAGCCCTAAACGGAGATCCAGGAGTCCCCATAGGAAATGGTCTGACAGTTCCAAGCAAGATG ACAGCCCTTCCGGAGCTTCTTATGGCCAAGACTACGATCTTAGCCCCCCAAGGTCTCACACTTCTAGCAA CTATGACTCCTACAAGAAGAGTCCTGGAAGTACCTCAAGAAGGCAGTCAATCAGCCCACCTTACAAAGAG CCTTCTGCTTACCAGTCCAGCACTCGGTCACCCAGTCCTTACAGCCGACGACAGAGGTCTGTGAGTCCCT ATAGCCGGAGACGGTCCTCCAGCTATGAAAGGAGCGGCTCTTACAGCGGGAGATCACCCAGCCCCTATGG CCGAAGGCGATCAAGCAGCCCTTTCCTGAGCAAGAGGTCTCTGAGTCGGAGTCCACTCCCCAGTAGGAAA TCCATGAAGTCCAGAAGTAGAAGTCCTGCATATTCAAGACACTCATCTTCTCATAGTAAAAAGAAGCGAT CCGGGTCACGCAGTCGTCATTCCAGTATCTCACCTGTCAGGCTTCCATTGAATTCCAGCCTGGGAGCTGA ACTCAGTAGAAAAAAGAAGGAAAGAGCAGCAGCTGCTGCAGCAGCAAAAATGGATGGAAAAGAGTCCAAG AGTTCACCTATAATTTTGCCTAAAAAAGAGAAACTTGAGGTGAAGGAGTCAGGGTTAGAGTCTAAAAAGT TACCCAGAAGTATAAAATCAGAAAAATCGACCCCAGATACTGAACTGGTTACTGTAGCACATTCAAACCC AGAGGTAAAACATTGTTTAGACACAGGGAAGGTTAGGTTGGATGAGAACTTGCAGAAGCATCCTGCTAAG GATTTGAAAGCACAGGGAACAAAGGACGTTAAACCTGTAGCACCGAAAGAGGTGATTGTTACTTCAAAGG AGACAGAGACATCAGAAAAGGAGACCCTTCCACCTCTTCCCACAATTACTTCTCCACCCCCTTTACCAGC TACTACCCCTCCACCTCAGACACCCCCTTTGCCACCTTTGCCTCCACTACCAGCTATTCCGCTGCAGCCA CCTCTGCCTCCTCCCCAACCACCATTTAGTCAAGTTCCTGTTTCAAGTACTTCAATTTTACCCTCTTCTC CTCACCCAAGGACATCTACTCTATCCTCTCAGACAAATTCTCAGCCCCCTGTACAGGTTTCTATGAAGAC TCAAGTATCTATAACAGCTGCTATTCCACATCTGAAGACTTCAACATTGCCTCCTCTGCCCCTCCCTCCC CTATTACCTGGAGATGATGACATGGATAGTCCAAAAGAAACACTTCCTTCAAAGCCTGCAAAGAAAGAGA AGGAACAGAGGACTCGCCACTTGCTTACAGACTTGCCTCTTCCTCCTGAGCTACCAGGAGGAGATCCATC GCCTCCAGATTCTCCAGAGCCAAAGGCAATTACACCACCTCAACAACCATATAAAAAGAGACCAAAAATT TGTTGTCCACGTTATGGAGAAAGAAGACAAACAGAAAGTGATTGGGGGAAGCGCTGTGTGGACAAGTTTG ACATTATTGGGATTATTGGAGAGGGGACCTATGGCCAAGTATATAAAGCCAAGGACAAAGACACAGGAGA ACTAGTAGCTCTGAAGAAGGTTCGGCTGGACAATGAGAAAGAGGGCTTCCCAATCACAGCCATCCGGGAG ATCAAGATTCTTCGTCAATTAGTTCACCAGAGTGTTGTAAACATGAAGGAAATTGTCACAGACAAACAAG ATGCACTGGATTTCAAGAAGGACAAAGGTGCCTTTTACCTTGTATTTGAATATATGGACCATGACTTAAT GGGACTGCTTGAATCAGGTTTGGTGCACTTTTCTGAGGACCATATCAAGTCATTTATGAAACAGCTAATG GAAGGACTGGATTACTGTCACAAAAAGAATTTCCTCCATCGGGATATTAAATGTTCTAACATTTTGCTGA ATAACAGCGGGCAAATCAAACTGGCAGATTTTGGACTTGCTCGGCTCTATAACTCTGAAGAGAGTCGCCC TTACACAAACAAAGTCATCACTCTTTGGTATCGACCTCCAGAGCTTCTTCTTGGAGAGGAAAGATACACA CCAGCCATTGATGTTTGGAGCTGTGGGTGCATCCTTGGAGAACTGTTCACAAAGAAACCTATTTTTCAAG CCAATTTAGAACTGGCTCAGCTAGAACTTATCAGTCGTCTCTGTGGTAGTCCTTGTCCAGCAGTGTGGCC TGATGTTATCAAGCTGCCCTACTTCAACACCATGAAACCGAAGAAGCAATACAGGAGACGCCTAAGAGAA GAATTCTCTTTCATTCCTTCAGCGGCACTTGATCTATTGGACCACATGCTGACACTGGATCCTAGCAAGA GGTGCACAGCTGAACAGACCCTACAGAGTGACTTTCTTAAAGATGTGGAACTCAGCAAAATGGCACCTCC AGACCTACCTCACTGGCAGGATTGCCATGAATTGTGGAGTAAGAAACGTCGACGGCAGCGACAGAGTGGT ATTGTGATAGAAGATCCACCTCCGTCCAAAGCTTCTAGAAAAGAAACTACCTCAGGGACAACAGCTGAGC CTGTGAAAAACAATAGCCCAGCACCACCTCAGCCTGCTCCTGTCAAGGCAGAGCCTGGTCCAGGGGATGC AGTAGGCCTTGGTGACATCACACAGCAGTTGAATCAAAGTGAATTGGCAGTGTTATTAAACCTGCTTCAG AGCCAAACTGACCTGAGCATCCCTCAGATGGCACAGCTGCTTAATATCCACTCCAATCCAGAGATGCAAC AGCAGCTTGAAGCCTTGAATCAGTCTATTAGTGCACTGACTGAAGCCAGTTCCCAGCAGCAGGACTCAGA ATCCATAGCCCCTGAAGAATCATTGAAGGAGGTACCTTCTGTACCTGTGGTCCTGCCTCCTGCTGAACAG ACAACTCCTGAAGCTTCAAACACACCAGCTGACATGCAGAATGTGTTGGCAGTTCTCTTGAGTCAGCTGA TGAAAACCCAAGAGCCAGCAGGTAACCTGGAGGAAAACACCAATGACAAGAATAGTGGGCCACAGGGGCC CCGAAGAACTCCTACAATGCCACAGGAGGAGGCAGCAGAGAAGAGGCCCCCTGAGCCCCCTGGACCTCCA CCGCCGCCACCTCCACCCCCTCTGGTTGAAGGCGATCTTTCCAGCGCCCCCCAGGAGTTGAATCCCGCCG TGACAGCCGCCTTGCTGCAACTTTTATCCCAGCCTGAAGCAGAGCCTCCTGGCCACCTGCCACATGAGCA CCAGGCCTTGAGACCAATGGAATACTCCACCCGATCCCATCCAAACAGGACTTACGGAAATACTGATGGG CCTGAGACAGGGTTCAGTTCCGCTGACACTGATGAACGCAGTTCTGGTCCAGCCTTGACAGAATCTTTGG TTCAGACCCCGGTGAAGAACAGGACCTTCTCAGGCTCTGTGAGCCACCTTGGGGAGTCCAACAGCTACCA GGGCACAGGGTCAGTGCAGTTCCCAGGGGACCAGGACCTCCGTTTTACCAGGGTTCCCTTAGCATTACAC TCAGTGGTTGGGCAACCATTCCTCAAGTCTGAGGGAAATAGCAACTCTGTGGTACATGCAGAGACCAAAT TGCAAAACTATGGGGAGCTGGGACCGGGAACCACTGGGGCCAACAGCTCAGGAACAACGCTTCAGTGGGG GGGCCCAGCTCAGTCTTATGGAAAACCCTACAGGGGGGCTGCAAGAGTCCTACCACGAGGGGGAAGAGGG AGAGGAGTTCCTTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR215624 representing NM_001109628
Red=Cloning site Green=Tags(s) MPNSERHGGKKDGSGGASGTSQPSSGGGSSNSRERHRLVSKHKRHKSKHSKDVGLVTPEAASLGTIIKPL VEYDDISSDSDTFSDDTAFKSDRRENEERRGTDRSDRLHRHRHHQHRRSRDLLKTKQTEKEKNQEVSKSG SMKDRVSGSSKRSVEGSDDYGKAQLSKSGSKESRSSKMHKEKTRKERELKSGYKDRSKSHRKRETPKSYK TVASPKRRSRSPHRKWSDSSKQDDSPSGASYGQDYDLSPPRSHTSSNYDSYKKSPGSTSRRQSISPPYKE PSAYQSSTRSPSPYSRRQRSVSPYSRRRSSSYERSGSYSGRSPSPYGRRRSSSPFLSKRSLSRSPLPSRK SMKSRSRSPAYSRHSSSHSKKKRSGSRSRHSSISPVRLPLNSSLGAELSRKKKERAAAAAAAKMDGKESK SSPIILPKKEKLEVKESGLESKKLPRSIKSEKSTPDTELVTVAHSNPEVKHCLDTGKVRLDENLQKHPAK DLKAQGTKDVKPVAPKEVIVTSKETETSEKETLPPLPTITSPPPLPATTPPPQTPPLPPLPPLPAIPLQP PLPPPQPPFSQVPVSSTSILPSSPHPRTSTLSSQTNSQPPVQVSMKTQVSITAAIPHLKTSTLPPLPLPP LLPGDDDMDSPKETLPSKPAKKEKEQRTRHLLTDLPLPPELPGGDPSPPDSPEPKAITPPQQPYKKRPKI CCPRYGERRQTESDWGKRCVDKFDIIGIIGEGTYGQVYKAKDKDTGELVALKKVRLDNEKEGFPITAIRE IKILRQLVHQSVVNMKEIVTDKQDALDFKKDKGAFYLVFEYMDHDLMGLLESGLVHFSEDHIKSFMKQLM EGLDYCHKKNFLHRDIKCSNILLNNSGQIKLADFGLARLYNSEESRPYTNKVITLWYRPPELLLGEERYT PAIDVWSCGCILGELFTKKPIFQANLELAQLELISRLCGSPCPAVWPDVIKLPYFNTMKPKKQYRRRLRE EFSFIPSAALDLLDHMLTLDPSKRCTAEQTLQSDFLKDVELSKMAPPDLPHWQDCHELWSKKRRRQRQSG IVIEDPPPSKASRKETTSGTTAEPVKNNSPAPPQPAPVKAEPGPGDAVGLGDITQQLNQSELAVLLNLLQ SQTDLSIPQMAQLLNIHSNPEMQQQLEALNQSISALTEASSQQQDSESIAPEESLKEVPSVPVVLPPAEQ TTPEASNTPADMQNVLAVLLSQLMKTQEPAGNLEENTNDKNSGPQGPRRTPTMPQEEAAEKRPPEPPGPP PPPPPPPLVEGDLSSAPQELNPAVTAALLQLLSQPEAEPPGHLPHEHQALRPMEYSTRSHPNRTYGNTDG PETGFSSADTDERSSGPALTESLVQTPVKNRTFSGSVSHLGESNSYQGTGSVQFPGDQDLRFTRVPLALH SVVGQPFLKSEGNSNSVVHAETKLQNYGELGPGTTGANSSGTTLQWGGPAQSYGKPYRGAARVLPRGGRG RGVPY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001109628 |
| ORF Size | 4425 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001109628.1, NP_001103098.1 |
| RefSeq Size | 7642 bp |
| RefSeq ORF | 4428 bp |
| Locus ID | 69131 |
| UniProt ID | Q14AX6 |
| MW | 163.2 kDa |
| Gene Summary | Cyclin-dependent kinase that phosphorylates the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A), thereby acting as a key regulator of transcription elongation. Regulates the expression of genes involved in DNA repair and is required for the maintenance of genomic stability. Preferentially phosphorylates 'Ser-5' in CTD repeats that are already phosphorylated at 'Ser-7', but can also phosphorylate 'Ser-2'. Required for RNA splicing, possibly by phosphorylating SRSF1/SF2. Involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogen inhibitors.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224531 | Cdk12 (untagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 2, (10ug) |
CNY 18,910.00 |
|
| MG215624 | Cdk12 (tGFP-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12) transcript variant 2, (10ug) |
CNY 16,250.00 |
|
| MR215624L3 | Lenti ORF clone of Cdk12 (Myc-DDK-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 2 |
CNY 16,630.00 |
|
| MR215624L4 | Lenti ORF clone of Cdk12 (mGFP-tagged) - Mouse cyclin-dependent kinase 12 (Cdk12), transcript variant 2 |
CNY 16,630.00 |
