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Nphp4 (NM_153424) Mouse Tagged ORF Clone
CAT＃: MR216552
- TrueORF®
Nphp4 (Myc-DDK-tagged) - Mouse nephronophthisis 4 (juvenile) homolog (human) (Nphp4)
ORF Plasmid: tGFP
"NM_153424" in other vectors (4)
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CNY 14,250.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930564O18Rik; nmf192 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216552 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTGACTGGCACAGAGCCTTCACCCAGAACACACTGGTCCCTCCCCACCCACAGAGAGCGAGGCAGC TTGGGAAGGAGTCCACGGCATTCCAGTGCATCCTGAAGTGGCTTGACGGGCCACTCATTAAGCAGGGAAT CCTAGACATGCTGTCGGAGCTTGAATGCCATCTGCGAGTGACCCTCTTCGATGTCACCTACAAACATTTC TTTGGGAGGACGTGGAAAACCACAGTGAAGCCCACCAATCAACCCTCTAAGCAGCCACCTAGGATCACCT TCAATGAGCCCTTGTACTTCCACACGACCTTGAGCCACCCCAGCATCGTGGCTGTGGTAGAAGTGGTCAC CGAAGGCAGGAAGAGGGACGGCACCCTGCAGCTGCTGTCCTGTGGGTTTGGAATTCTTCGCATCTTTGGC AATAAGCCAGAATCTCCCACCTCTGCAGCCCAGGACAAACGGTTGAGGCTGTATCATGGCACCCCAAGAG CCCTCCTGCACCCACTTCTCCAGGATCCCATAGAACAGAACAAGTTCATGAGGCTGATGGAGAATTGTAG CCTGCAGTACACCCTGAAGCCACACCCACCCCTGGAGCCCGCCTTCCACCTGCTCCCTGAGAACCTCCTG GTGTCTGGTTTCCAGCAGATCCCTGGCCTTCTCCCGCCTCATGGGGACACGGGTGATGCCCTGCGGAAGC CTCGCTTCCAGAAGCCCACCACATGGCACTTAGATGACCTCTTCTTCACCCTGTACCCTTCCCTGGAGAA GTTTGAGGAGGAACTTGTGCAGCTGCTCATCAGCGACCGGGAGGGGGTCGGCCTGCTGGACAGCGGCACC CTCGAGGTCCTGGAGCGGCGCCTGCACGTCTGCGTCCACAACGGGCTGGGCTTCGTGCAGAGGCCACAGG TGGTTGTGCTTGTGCCTGAGATGGACGTGGCCTTGACCCGCTCGGCCAGCTTCAGCAGGAAAATCAGTGC CTCTTCCAAGAACAGCTCTGGAAATCAGGCTCTAGTTTTGAGAAGCCACCTGCGACTCCCGGAGATGGTC AGTCACCCAGCCTTCGCCATCGTCTTCCAGCTGGAGTACGTGTTCAACAGCCCCTCTGGAGCGGATGGCG GTGCCAGCTCGCCCACCTCAATATCCAGCGTGGCGTGCATGCACATGGTCCGATGGGCTGTTTGGAACCC CGATCTGGAAGTGGGCCCAGGAAAGGTGACGCTGCCTCTTCAGGGTGGGGTTCAGCAAAACCCCTCACGC TGTCTGGTCTATAAGGTGCCTTCAGCTAGCATGAGCTCCGAGGAGGTGAAGCAGGTGGAGTCCGGAACCA TCCAGTTCCAGTTCTCACTGAGCTCAGACGGCCCCACAGAGCATGCCAACGGCCCCAGGGTGGGGCGTCG GTCCTCCCGGAAGATGCCGGCATCCCCTTCAGGCACCCCAGCACCAGCTGCCCGGGATCTTGCTGCAACA CAGGACTCTCCTGTGGGCCCAGGGCTCTCGCTCTCCCAGCTGACGGCTTCCCCGCTGTCCCCGGCTCTGC AAAGCTCTTCCAAGCCCCCTCTTCAGCCCCCGGACAGCTCTCAGTCCCCAGAGGGTCCCCAGCTCCAGGC CGAATCTGTATTAGAGAGCAGGGTCTCTCACCTGGAAGCCGACCTGAGCCAGCCGGCCTCGCTCCAGGGA ACGCCTGCTGTCGAGCATCTACAGGAACTTCCCTTCACTCCTCTGCATGCCCCCATCGTCGTGGGAGCAC AGACCAGGAGCTCTAGAAGCCAGCTCTCCAGAGCTGCGATGGTGCTTCTGCAGTCTTCTGGCTTCCCCGA GATCCTTGATGCCAGCCAGCAGCCCGTGGAGGCCGTCAACCCCATAGACCCCGTGAGGTTCAACCCTCAG AAGGAGGAGTCGGACTGTCTTCGAGGGAATGAGATTGTGCTGCAGTTTCTTGCCTTCAGCAGAGCCGCCC AGGACTGCCCAGGAACGCCGTGGCCACAGACTGTGTATTTCACCTTCCAGTTTTACCGTTTCCCACCCGA GACCACGCCACGCCTGCAGCTGGTCAAGCTGGATGGGACGGGCAAGAGCGGCTCTGGCTCCCTGTCCCAC ATCCTCGTCCCCATTAATAAAGATGGCTCCTTTGATGCTGGCTCCCCCGGCCTCCAGCTGAGGTACATGG TGGATCCCGGGTTCCTGAAGCCAGGAGAGCAGCGCTGGTTCGCCCACTACCTGGCTGCTCAGACGCTGCA GGTCGACGTCTGGGATGGAGACTCCTTGCTCCTCATTGGGTCTGCTGGCGTCCAGATGAAGCACCTCCTC CGCCAAGGGCGGCCAGCTGTTCAGGTCTCACACGAACTGGAGGTCGTGGCCACTGAGTACGAACAGGAAA TGATGGCCGTGAGCGGCGATGTGGCTGGCTTCGGCAGCGTCAAGCCCATCGGAGTCCACACGGTAGTGAA GGGCCGCCTGCACCTGACCTTGGCCAACGTGGGTCACGCTTGTGAGCCGAGAGCAAGAGGTTCCAACTTG CTGCCGCCATCCAGGTCTCGGGTCATCTCAAATGATGGAGCCAGCTTCTTCTCTGGGGGAAGCCTCCTCA TACCCGGAGGCCCCAAGCGAAAGCGTGTGGTACAGGCCCAGAGGCTGGCTGATGTGGACAGCGAGCTGGC CGCCATGCTGCTGACACACACTCGAGCTGGCCAGGGGCCCCAGGCTGCAGGCCAGGAGGCAGACGCTGTG CACAAGCGGAAGCTGGAGAGGATGCGGCTGGTGCGCCTGCAGGAGGCTGGAGGGGACTCTGACAGTCGCA GGATCAGCTTGCTGGCCCAACACAGTGTCCGTGCACAGCACTCACGGGACTTGCAGGTCATTGATGCCTA CCGGGAACGTACCAAGGCCGAGAGCATTGCCGGAGTGCTAAGCCAGGCCATCACCACCCACCACACGCTG TACGCTACACTGGGCACCGCTGAGTTCTTCGAGTTTGCACTTAAAAACCCACACAACACTCAGCACACGG TGGCCATCGAGATCGACAGCCCTGAGCTCAGTATCATCCTGGACAGCCAGGAGTGGAGGTACTTCAAGGA GGCCACCGGCCTGCACACGCCCCTGGAGGAGGATATGTTCCACCTGCGTGGGAGCCTGGCACCCCAGCTC TACCTGCGACCCCGGGAGACTGCCCACATCCCCCTTAAGTTCCAGAGCTTCTCCGTGGGCCCTCTTGCTC CGACACAGGCCCCTGCTGAGGTGATCACAGAGAAGGACGCAGAGTCTGGACCCCTTTGGAAGTGCAGCGC CATGCCTACGAAACACGCCAAGGTCCTTTTCCGGGTGGAGACTGGCCAGCTCATTGCTGTGCTCTGCCTG ACCGTGGAGCCCCAGCCCCACGTGGTGGACCAGGTTTTCCGCTTCTACCACCCAGAGCTCACGTTCCTGA AGAAGGCCATCCGCCTGCCTCCCTGGCACACGCTTCCAGGTGCTCCTGTGGGGATGCCTGGCGAGGATCC CCCAGTGCATGTTCGATGCAGTGATCCAAATGTCATCTGTGAGGCCCAGAACGTGGGCCCCGGTGAGCCG AGAGATGTGTTCCTTAAGGTGGCCAGTGGTCCAAGCCCGGAGATCAAAGACTTCTTTGTCGTCATCTATG CGGATCGCTGGCTGGCGGTGCCCGTGCAGACGTGGCAGGTCTGCCTCCACTCCCTGCAGCGCGTGGATGT TTCCTGTGTCGCAGGACAGCTGACCCGTCTGTCCCTCGTCCTTCGGGGGACACAGACCGTTAGGAAAGTG AGAGCTTTCACCTCCCACCCCCAGGAGCTGAAGACAGACCCTGCCGGTGTCTTTGTGTTGCCACCTCATG GGGTACAGGACCTGCATGTGGGTGTGAGGCCAAGGAGGGCTGGCAGCCGCTTTGTCCATCTCAACCTAGT GGACATAGACTACCATCAGCTGGTTGCGTCATGGCTTGTGTGCCTCTCCTGCCGCCAGCCACTCATCTCC AAGGCCTTCGAGATCACCATGGCAGCAGGGGATGAGAAGGGCACCAACAAGCGCATCACCTACACCAACC CCTATCCTTCTCGGAGGACCTACCGCCTGCACAGTGACCGTCCCGAGCTGTTGCGCTTCAAGGAGGACTC CTTCCAGGTGGCGGGAGGAGAGACCTACACCATCGGCCTGCGGTTCCTGCCAAGTGGCAGCGCCGGGCAG GAGGAGATCCTGATCTACATCAATGACCACGAGGACAAGAATGAGGAGACATTCTGTGTGAAGGTCCTCT ACCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216552 protein sequence
Red=Cloning site Green=Tags(s) MGDWHRAFTQNTLVPPHPQRARQLGKESTAFQCILKWLDGPLIKQGILDMLSELECHLRVTLFDVTYKHF FGRTWKTTVKPTNQPSKQPPRITFNEPLYFHTTLSHPSIVAVVEVVTEGRKRDGTLQLLSCGFGILRIFG NKPESPTSAAQDKRLRLYHGTPRALLHPLLQDPIEQNKFMRLMENCSLQYTLKPHPPLEPAFHLLPENLL VSGFQQIPGLLPPHGDTGDALRKPRFQKPTTWHLDDLFFTLYPSLEKFEEELVQLLISDREGVGLLDSGT LEVLERRLHVCVHNGLGFVQRPQVVVLVPEMDVALTRSASFSRKISASSKNSSGNQALVLRSHLRLPEMV SHPAFAIVFQLEYVFNSPSGADGGASSPTSISSVACMHMVRWAVWNPDLEVGPGKVTLPLQGGVQQNPSR CLVYKVPSASMSSEEVKQVESGTIQFQFSLSSDGPTEHANGPRVGRRSSRKMPASPSGTPAPAARDLAAT QDSPVGPGLSLSQLTASPLSPALQSSSKPPLQPPDSSQSPEGPQLQAESVLESRVSHLEADLSQPASLQG TPAVEHLQELPFTPLHAPIVVGAQTRSSRSQLSRAAMVLLQSSGFPEILDASQQPVEAVNPIDPVRFNPQ KEESDCLRGNEIVLQFLAFSRAAQDCPGTPWPQTVYFTFQFYRFPPETTPRLQLVKLDGTGKSGSGSLSH ILVPINKDGSFDAGSPGLQLRYMVDPGFLKPGEQRWFAHYLAAQTLQVDVWDGDSLLLIGSAGVQMKHLL RQGRPAVQVSHELEVVATEYEQEMMAVSGDVAGFGSVKPIGVHTVVKGRLHLTLANVGHACEPRARGSNL LPPSRSRVISNDGASFFSGGSLLIPGGPKRKRVVQAQRLADVDSELAAMLLTHTRAGQGPQAAGQEADAV HKRKLERMRLVRLQEAGGDSDSRRISLLAQHSVRAQHSRDLQVIDAYRERTKAESIAGVLSQAITTHHTL YATLGTAEFFEFALKNPHNTQHTVAIEIDSPELSIILDSQEWRYFKEATGLHTPLEEDMFHLRGSLAPQL YLRPRETAHIPLKFQSFSVGPLAPTQAPAEVITEKDAESGPLWKCSAMPTKHAKVLFRVETGQLIAVLCL TVEPQPHVVDQVFRFYHPELTFLKKAIRLPPWHTLPGAPVGMPGEDPPVHVRCSDPNVICEAQNVGPGEP RDVFLKVASGPSPEIKDFFVVIYADRWLAVPVQTWQVCLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRKV RAFTSHPQELKTDPAGVFVLPPHGVQDLHVGVRPRRAGSRFVHLNLVDIDYHQLVASWLVCLSCRQPLIS KAFEITMAAGDEKGTNKRITYTNPYPSRRTYRLHSDRPELLRFKEDSFQVAGGETYTIGLRFLPSGSAGQ EEILIYINDHEDKNEETFCVKVLYQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153424 |
| ORF Size | 4278 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153424.2, NP_700473.2 |
| RefSeq Size | 5047 bp |
| RefSeq ORF | 4278 bp |
| Locus ID | 260305 |
| UniProt ID | P59240 |
| MW | 157.3 kDa |
| Gene Summary | Involved in the organization of apical junctions; the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis (By similarity). Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1 (By similarity). In cooperation with INVS may downregulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling (By similarity). Acts as negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224441 | Nphp4 (untagged) - Mouse nephronophthisis 4 (juvenile) homolog (human) (Nphp4), (10ug) |
CNY 14,632.00 |
|
| MG216552 | Nphp4 (tGFP-tagged) - Mouse nephronophthisis 4 (juvenile) homolog (human) (Nphp4), (10ug) |
CNY 15,680.00 |
|
| MR216552L3 | Lenti ORF clone of Nphp4 (Myc-DDK-tagged) - Mouse nephronophthisis 4 (juvenile) homolog (human) (Nphp4) |
CNY 16,150.00 |
|
| MR216552L4 | Lenti ORF clone of Nphp4 (mGFP-tagged) - Mouse nephronophthisis 4 (juvenile) homolog (human) (Nphp4) |
CNY 16,150.00 |
