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Rnf7 (NM_011279) Mouse Tagged Lenti ORF Clone
CAT＃: MR220386L3
- LentiORF®
Lenti ORF clone of Rnf7 (Myc-DDK-tagged) - Mouse ring finger protein 7 (Rnf7)
"NM_011279" in other vectors (4)
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CNY 4,750.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Rbx2; SAG |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR220386).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011279 |
| ORF Size | 342 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011279.2, NP_035409.1 |
| RefSeq Size | 1132 bp |
| RefSeq ORF | 342 bp |
| Locus ID | 19823 |
| UniProt ID | Q9WTZ1 |
| Gene Summary | Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (By similarity). CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity). Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209319 | Rnf7 (untagged) - Mouse ring finger protein 7 (Rnf7), (10ug) |
CNY 3,990.00 |
|
| MG220386 | Rnf7 (tGFP-tagged) - Mouse ring finger protein 7 (Rnf7), (10ug) |
CNY 2,850.00 |
|
| MR220386 | Rnf7 (Myc-DDK-tagged) - Mouse ring finger protein 7 (Rnf7) |
CNY 1,200.00 |
|
| MR220386L4 | Lenti ORF clone of Rnf7 (mGFP-tagged) - Mouse ring finger protein 7 (Rnf7) |
CNY 4,750.00 |
