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Otud4 (NM_001081164) Mouse Tagged ORF Clone
CAT＃: MR222438
- TrueORF®
Otud4 (Myc-DDK-tagged) - Mouse OTU domain containing 4 (Otud4)
ORF Plasmid: tGFP
"NM_001081164" in other vectors (4)
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CNY 7,712.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930431L18Rik; AI449692; D8Ertd69e; mKIAA1046 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222438 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCAGCCGTCGGCGCGCCCGACGGCGTGGACCAGGGCGGCGTGGGGCCGCTGGAGGATGAGACGC CCATGGACGCCTATCTGCGCAAACTGGGCTTGTATCGGAAATTGGTCGCCAAGGACGGGTCGTGCTTGTT CCGGGCTGTGGCCGAGCAGGTGTTGCACTCTCAGTCTCGGCATGTGGAGGTTAGGATGGCCTGTATCCGC TACCTTCGAGAGAACAGAGAGAAATTTGAAGCGTTTATAGAAGGGTCATTTGAAGAATATTTAAAACGTT TGGAAAATCCACAGGAATGGGTAGGACAAGTGGAAATAAGTGCCCTTTCACTTATGTACAGGAAAGATTT TGTAATATATCAGGAGCCAAATGTTTCTCCTTCACATGTAACTGAAAATAATTTTCCTGAGAAGGTGTTA CTGTGTTTTTCAAATGGAAATCATTATGACATTGTCTATCCCATAACATATAAAGATAGTTCTGCTATGT GTCAGTCTCTCCTTTATGAGTTGCTGTATGAGAAGGTATTCAAAACTGATGTTAGTAAAATCATGATGGG ACTAGAAGCCTCTGAGGTGGCTGAGGAGAGCAACAGTGAGATATCAGACTCTGAGGACGACAGCTGCAAG AGTAAAAGTACTGCTGCTACTGATGTGAATGGATTTAAACCCTCAGGCAGTGAGAACCCTAAGAACAATG GGAACTCAGCTGACCTTCCTTTGTCCAGAAAGGTTCTTAAGTCACTCAACCCAGCAGTCTATAGAAATGT GGAGTATGAAATTTGGTTGAAGTCTAAACAAGCTCAACAAAAACGTGATTATTCCATTGCTGCTGGCTTA CAGTATGAAGTTGGAGATAAATGCCACCAGGTTAGATTGGATCATAATGGGAAATTATCTAATGCAGACA TTCATGGGGTTCACTCTGAGAATGGACTGGTTTTGTCTGAAGAACTGGGGAAAAAACATACACCGAAGAA CCTCAAGCCACCTCCCCCAGAAAGCTGGAACACGGTGTCAGGAAAGAAGATGAAAAAACCTAATTCTGGG CAAAATTTCCATTCAGATACAGATTACAGAGGGCCAAAGAATCTAAACAAGCCAATCAAAGCCCCATCTG CACTACCTCCTCGACTCCAGCATCCTTCATCGGGTGTAAGACAGCATGCATTCTCCAGTCATTCTACAGG GTCCCAGTCTCAGAAATCCTCCAGTGAGCATAAGAATCTAAGTAGGATGCCCTCACAGATCACAAGAAAA CCTGACCGTGAAAGGGCTGAGGACTTTGATCACGTGAGTCGTGAATCTTACTATTTTGGCCTCTCCCCAG AAGAACGCAGAGAGAAGCAAGCTATTGAAGAGTCTCGTTTACTGTATGAGATTCAGAACCGGGATGAACA GGCTTTCCCTGCCCTTTCTAGTTCATCAGTCAGTCAGTCACCTTCTCAGAATAGCAATGCGTGTGTCCCA AGGAAGTCTTCACATGCAAGGGACAGGAAAGGAAGCATGCGGAGAGCAGACGCAGAGGAACGAAAGGACA AAGACTCTCTACGTGGCCATACTCATGTGGATAAAAAACCGGAGCCAAGCACACTGGAGATCAGCGATGA TAAATGTACAAGAGTTTCATCACCATCTAAGTCAAAGAAAGAGTGCCCATCTCCTGTAGAACAAAAGCCA GCAGAACATATACCTTTGTCAAATCCAGCTCCCCTTCTAGTTTCTCCAGAAGTACATCTCACTCCTGCGG TGCCTTCTTTACCAGCCACTGTGCCAGCCTGGCCAAGTGAACCTACAACTTTCGGACCAACAGGTGTCCC TGCTCAGATTCCCATTTTGTCAGTGACACAGACCACTGGACCTGATGCTGCCGTGTCACAAGCGCATTTA ACACCTTCTCCGGTTCCTGTGTCAATTCAGGCAGTTAACCAGCCCTTGATGCCTTTGCCTCAGACAATGA GCCTCTATCAAGACCCCCTCTATCCTGGGTTTCCTTGTAGTGAGAAGGGAGATCGAGCCATTGCACCACC TTATTCACTGTGTCAGACCGGGGAGGACCTGCCTAAAGATAAGAATATTCTTCGATTCTTCTTCAATCTC GGTGTAAAGGCATATAGTTGTCCTATGTGGGCCCCACATTCTTACCTATATCCTCTGCACCAGGCCTATA TGGCAGCCTGCAGGATGTACCCAAAGGTCCCTGTTCCCGTGTATCCTCAGAATACTTGGTTCCAAGAAGC CCCTCCTGCTCAGAGTGAAAGTGACTGTCCTTGCACCGATGCCCACTACTCTCTGCACCCCGAGGCCAGT GTTAATGGTCAGATGCCACAGGCAGAGATGGGACCGCCTGCATTTGCATCACCTCTGGTTATCCCTCCAT CTCAGGTGTCTGAAGGTCATGGACAATTGTCTTACCAACCTGAACTGGAGTCTGAGAACCCAGGGCAGCT TCTTCATGCTGAATATGAAGAGTCACTTAGTGGCAAGAACATGTACCCACAACAGTCTTTTGGGCCTAAC CCATTTTTAGGTCCTGTTCCCATTGCACCTCCTTTCTTCCCTCATGTTTGGTATGGGTATCCTTTTCAGG GATTCGTAGAAAATCCTGTAATGAGGCAAAATATTGTCCTGCCCCCTGATGATAAAGGAGAATTGGATTT GCCTTTGGAGAATCTAGATCTGTCTAAAGAATGCGATTCTGTCTCAGCAGTAGATGAGTTCCCAGACGCC AGAGTTGAAGGTGCACATTCTCTGTCTGCAGCGAGTGTGAGCAGCAAGCACGAAGGCCGAGTGGAGCAGT CGTCCCAGACCCGGAAGGCAGACATAGACTTGGCTTCAGGTTCTTCTGCAGTGGAAGGAAAGGGTCATCC TCCCACTCAGATTCTAAACAGAGAAAGAGAACCTGGGTCTGCTGAACCTGAGCCTAAGAGGACCATTCAA AGTCTGAAAGAAAAACCAGAGAAAGTAAAAGATCCCAAGACTGCTGCTGATGTGGTCAGCCCTGGGGCCA ATTCTGTGGATAGATTGCAAAGACCAAAAGAAGAGAGTTCAGAAGATGAGAATGAAGTATCTAATATTTT GAGAAGTGGCAGATCCAAGCAGTTTTATAATCAAACTTACGGAAGCAGGAAGTACAAAAGTGATTGGGGC TCTTCTGGTCGAGGTGGCTATCAACACGTGAGAGGCGAGGAGTCCTGGAAAGGGCAGCCAAATCGAAGCC GGGATGAAGGTTATCAGTACCATCGACATGTTAGAGGACGCCCATACAGGGGAGATAGGAGGAGATCAGG GATGGGAGATGGCCACAGGGGACAACACACT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222438 protein sequence
Red=Cloning site Green=Tags(s) MEAAVGAPDGVDQGGVGPLEDETPMDAYLRKLGLYRKLVAKDGSCLFRAVAEQVLHSQSRHVEVRMACIR YLRENREKFEAFIEGSFEEYLKRLENPQEWVGQVEISALSLMYRKDFVIYQEPNVSPSHVTENNFPEKVL LCFSNGNHYDIVYPITYKDSSAMCQSLLYELLYEKVFKTDVSKIMMGLEASEVAEESNSEISDSEDDSCK SKSTAATDVNGFKPSGSENPKNNGNSADLPLSRKVLKSLNPAVYRNVEYEIWLKSKQAQQKRDYSIAAGL QYEVGDKCHQVRLDHNGKLSNADIHGVHSENGLVLSEELGKKHTPKNLKPPPPESWNTVSGKKMKKPNSG QNFHSDTDYRGPKNLNKPIKAPSALPPRLQHPSSGVRQHAFSSHSTGSQSQKSSSEHKNLSRMPSQITRK PDRERAEDFDHVSRESYYFGLSPEERREKQAIEESRLLYEIQNRDEQAFPALSSSSVSQSPSQNSNACVP RKSSHARDRKGSMRRADAEERKDKDSLRGHTHVDKKPEPSTLEISDDKCTRVSSPSKSKKECPSPVEQKP AEHIPLSNPAPLLVSPEVHLTPAVPSLPATVPAWPSEPTTFGPTGVPAQIPILSVTQTTGPDAAVSQAHL TPSPVPVSIQAVNQPLMPLPQTMSLYQDPLYPGFPCSEKGDRAIAPPYSLCQTGEDLPKDKNILRFFFNL GVKAYSCPMWAPHSYLYPLHQAYMAACRMYPKVPVPVYPQNTWFQEAPPAQSESDCPCTDAHYSLHPEAS VNGQMPQAEMGPPAFASPLVIPPSQVSEGHGQLSYQPELESENPGQLLHAEYEESLSGKNMYPQQSFGPN PFLGPVPIAPPFFPHVWYGYPFQGFVENPVMRQNIVLPPDDKGELDLPLENLDLSKECDSVSAVDEFPDA RVEGAHSLSAASVSSKHEGRVEQSSQTRKADIDLASGSSAVEGKGHPPTQILNREREPGSAEPEPKRTIQ SLKEKPEKVKDPKTAADVVSPGANSVDRLQRPKEESSEDENEVSNILRSGRSKQFYNQTYGSRKYKSDWG SSGRGGYQHVRGEESWKGQPNRSRDEGYQYHRHVRGRPYRGDRRRSGMGDGHRGQHT TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081164 |
| ORF Size | 3324 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081164.1, NP_001074633.1 |
| RefSeq Size | 7324 bp |
| RefSeq ORF | 3321 bp |
| Locus ID | 73945 |
| UniProt ID | B2RRE7 |
| MW | 123.1 kDa |
| Gene Summary | Deubiquitinase which hydrolyzes the isopeptide bond between the ubiquitin C-terminus and the lysine epsilon-amino group of the target protein. May negatively regulate inflammatory and pathogen recognition signaling in innate immune response. Upon phosphorylation at Ser-202 and Ser-204 residues, via IL-1 receptor and Toll-like receptor signaling pathway, specifically deubiquitinates 'Lys-63'-polyubiquitinated MYD88 adapter protein triggering down-regulation of NF-kappa-B-dependent transcription of inflammatory mediators (PubMed:29395066). Independently of the catalytic activity, acts as a scaffold for alternative deubiquitinases to assemble specific deubiquitinase-substrate complexes. Associates with USP7 and USP9X deubiquitinases to stabilize alkylation repair enzyme ALKBH3, thereby promoting the repair of alkylated DNA lesions (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
OTUD1 Regulates Antifungal Innate Immunity through Deubiquitination of CARD9
,Chen, X;Zhang, H;Wang, X;Shao, Z;Li, Y;Zhao, G;Liu, F;Liu, B;Zheng, Y;Chen, T;Zheng, H;Zhang, L;Gao, C;,
Journal of immunology (Baltimore, Md. : 1950) 2021
,PubMed ID 33789983
[Otud4]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223591 | Otud4 (untagged) - Mouse OTU domain containing 4 (Otud4), (10ug) |
CNY 14,160.00 |
|
| MG222438 | Otud4 (tGFP-tagged) - Mouse OTU domain containing 4 (Otud4), (10ug) |
CNY 10,640.00 |
|
| MR222438L3 | Lenti ORF clone of Otud4 (Myc-DDK-tagged) - Mouse OTU domain containing 4 (Otud4) |
CNY 11,590.00 |
|
| MR222438L4 | Lenti ORF clone of Otud4 (mGFP-tagged) - Mouse OTU domain containing 4 (Otud4) |
CNY 11,590.00 |
