Bves (NM_024285) Mouse Tagged ORF Clone

CAT#: MR225843

  • TrueORF®

Bves (Myc-DDK-tagged) - Mouse blood vessel epicardial substance (Bves)

ORF Plasmid: DDK tGFP



  "NM_024285" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms mBVES; Pop1; Popdc1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR225843 representing NM_024285
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATTCCACGGAGTCCATCCCGCTGGCACAGTCAACTGTAGCAGGTTTTACATCTGAGTTAGAAAGTC
TCACACCCGTGCCTTCTAATGAGACCACTTGTGAGAACTGGCGAGAGATTCACCATCTGGTTTTTCATGT
AGCAAACGTTTGCTTTGCAGTTGGTTTGCTAATTCCCACCACTCTTCACCTCCATATGATACTGCTCAGA
GTGATGTTGTCTCTAGGATGTACCCTTTATGTGGTCTGGGCCACTCTCTACCGCTGTGCCTTGGATGTGA
TGATCTGGAACTCGGTGTTCTTGGGTATCAATATTTTGCATCTTTCATATCTTTTGTACAAGAAAAGACC
GGTCAAAATTGAAAAGGAGCTGGGTGGTGTCTACCATCGGTTGTTTGAACCACTCCGAGTCCCTCCGGAT
TTGTTTAGAAGGTTAACCGGACAGTTTTGTATGATCCAGACCTTGAAAAGGGGCCAGGTTTACGCCACAG
AGGACAAAACCTCAGTTGATGATCGTCTTAGTATTCTCCTAAAAGGAAGAATGAAGGTCTCCTATCGAGG
ACATTTTCTGCATAACATTTACCCGTGTGCCTTTATAGATTCTCCCGAATTCAGATCAACGCAGATGCAC
AAAGGCGAAAAATTCCAGGTCACCATTGTTGCAGATGATAACTGCAGGTTCTTATGCTGGTCAAGAGAAA
GACTGACTTACTTTCTGGAGTCAGAGCCTTTTCTGTATGAAATTTTTAGGTACCTTATAGGAAAAGACAT
TACAAATAAGCTGTACTCACTGAATGACCCTACTTTAAATGATAAGAAAGTTAAGAAGCTGGAGCCCCAG
ATGAGCCTCTGCACACAGATCTCCATGCTGGAGATGAGGAACAGTATCACCAGCTCCAGCGACGGCGAGG
ACGGCCTGCACCACTTTCTGCGGGGCTCCTCCAGCACGGCGTCTCTCCCCATGTCCTCCCCGCAGCAGCG
AGCCTCTGCCAAGATGAAGCCAATCGAGGAAGGGGTAGAAGATGACGATGAGGTCTTTGTGTCTCCAGAC
GCACTCAAAGTCCATCAGTTGCCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR225843 representing NM_024285
Red=Cloning site Green=Tags(s)

MNSTESIPLAQSTVAGFTSELESLTPVPSNETTCENWREIHHLVFHVANVCFAVGLLIPTTLHLHMILLR
VMLSLGCTLYVVWATLYRCALDVMIWNSVFLGINILHLSYLLYKKRPVKIEKELGGVYHRLFEPLRVPPD
LFRRLTGQFCMIQTLKRGQVYATEDKTSVDDRLSILLKGRMKVSYRGHFLHNIYPCAFIDSPEFRSTQMH
KGEKFQVTIVADDNCRFLCWSRERLTYFLESEPFLYEIFRYLIGKDITNKLYSLNDPTLNDKKVKKLEPQ
MSLCTQISMLEMRNSITSSSDGEDGLHHFLRGSSSTASLPMSSPQQRASAKMKPIEEGVEDDDEVFVSPD
ALKVHQLP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024285
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024285.3
RefSeq Size 1607 bp
RefSeq ORF 1077 bp
Locus ID 23828
UniProt ID Q9ES83
MW 41.5 kDa
Gene Summary Cell adhesion molecule involved in the establishment and/or maintenance of cell integrity. Involved in the formation and regulation of the tight junction (TJ) paracellular permeability barrier in epithelial cells (PubMed:16188940). Plays a role in VAMP3-mediated vesicular transport and recycling of different receptor molecules through its interaction with VAMP3 (PubMed:20057356). Plays a role in the regulation of cell shape and movement by modulating the Rho-family GTPase activity through its interaction with ARHGEF25/GEFT (PubMed:18541910). Induces primordial adhesive contact and aggregation of epithelial cells in a Ca(2+)-independent manner. Also involved in striated muscle regeneration and repair and in the regulation of cell spreading (PubMed:11839816). Important for the maintenance of cardiac function. Plays a regulatory function in heart rate dynamics mediated, at least in part, through cAMP-binding and, probably, by increasing cell surface expression of the potassium channel KCNK2 and enhancing current density (PubMed:26642364). Is a caveolae-associated protein important for the preservation of caveolae structural and functional integrity as well as for heart protection against ischemia injury (PubMed:24066022).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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