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Epha7 (NM_010141) Mouse Tagged ORF Clone
CAT＃: MR226270
- TrueORF®
Epha7 (Myc-DDK-tagged) - Mouse Eph receptor A7 (Epha7), transcript variant 1
ORF Plasmid: tGFP
"NM_010141" in other vectors (4)
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CNY 7,312.00
CNY 8,740.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cek11; Ebk; Ehk3; Hek11; Mdk1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226270 representing NM_010141
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTGTTCAAACTCGGTTCCCTTCGTGGATTATTTTGTGTTACATCTGGCTGCTTGGCTTTGCACACA CGGGGGAGGCGCAGGCTGCGAAGGAAGTACTATTACTGGACTCGAAAGCACAACAAACAGAATTGGAATG GATTTCCTCTCCACCCAGTGGGTGGGAAGAAATTAGTGGTTTGGATGAGAACTACACTCCGATAAGAACA TACCAGGTGTGCCAGGTCATGGAGCCCAACCAGAACAACTGGCTGCGGACTAACTGGATTTCTAAAGGCA ACGCACAAAGGATTTTTGTAGAATTGAAATTCACCTTGAGGGATTGTAATAGTCTTCCCGGAGTCCTGGG AACTTGCAAGGAAACGTTTAATTTGTACTATTATGAAACAGACTACGACACCGGCAGGAATATACGAGAA AACCTTTATGTTAAAATAGACACCATTGCTGCAGATGAAAGTTTCACACAAGGTGACCTTGGTGAAAGAA AGATGAAGCTGAACACTGAGGTGAGAGAGATTGGACCTTTGTCCAAAAAGGGATTCTATCTTGCCTTTCA GGATGTAGGGGCTTGCATAGCATTGGTTTCTGTCAAAGTGTACTACAAGAAGTGCTGGTCCATTGTTGAG AACTTAGCTGTCTTTCCAGATACAGTGACTGGTTCGGAATTTTCCTCCTTAGTCGAGGTCCGTGGGACAT GTGTCAGCAGTGCCGAGGAAGAGGCAGAAAATTCCCCCAGAATGCATTGCAGTGCAGAAGGAGAGTGGCT AGTACCCATTGGAAAATGCATCTGCAAAGCAGGCTATCAGCAAAAAGGGGACACTTGCGAACCCTGTGGC CGCAGGTTCTACAAATCTTCCTCTCAGGATCTCCAGTGTTCTCGTTGTCCAACCCACAGCTTCTCTGACC GAGAAGGATCATCCAGGTGTGAATGTGAAGATGGGTACTACAGAGCTCCTTCTGATCCACCATACGTTGC ATGCACGAGGCCTCCCTCTGCACCACAGAACCTTATTTTCAATATCAATCAAACGACTGTAAGTTTGGAA TGGAGTCCTCCGGCTGACAACGGGGGAAGAAACGATGTCACCTACAGAATACTGTGTAAGCGGTGCAGTT GGGAACAGGGAGAATGTGTGCCATGCGGAAGTAACATTGGATACATGCCCCAGCAGACGGGATTAGAGGA TAACTATGTCACTGTCATGGACCTACTTGCCCATGCAAATTACACTTTCGAAGTTGAAGCTGTAAATGGA GTTTCGGACTTAAGCAGATCCCAGAGGCTCTTCGCTGCTGTTAGCATCACCACCGGTCAAGCAGCTCCCT CGCAAGTGAGTGGAGTCATGAAGGAGCGAGTACTGCAGCGGAGTGTGCAGCTTTCCTGGCAGGAGCCGGA GCATCCCAATGGAGTCATCACGGAATATGAAATCAAGTATTATGAGAAAGATCAACGGGAAAGGACGTAC TCAACACTCAAAACCAAGTCCACCTCCGCCTCCATTAATAATCTGAAACCGGGAACAGTGTACGTCTTTC AGATCCGGGCGGTCACTGCTGCCGGTTATGGAAACTACAGCCCTAGGCTTGATGTTGCCACACTTGAGGA AGCTTCAGGTAAAATGTTTGAAGCGACAGCAGTCTCCAGTGAACAGAATCCTGTCATCATAATTGCTGTA GTGGCTGTAGCAGGGACCATCATCTTGGTGTTCATGGTGTTCGGCTTCATCATTGGAAGAAGGCACTGTG GTTATAGCAAGGCTGACCAAGAAGGGGATGAAGAACTCTACTTTCATTTTAAATTTCCAGGCACCAAAAC CTACATTGACCCTGAAACCTATGAGGACCCAAATAGAGCTGTCCATCAATTCGCCAAGGAGCTAGATGCC TCCTGTATTAAAATTGAGCGTGTGATTGGTGCAGGAGAATTTGGAGAAGTTTGCAGTGGTCGTTTGAAAC TTCCGGGGAAGAGAGATGTTGCAGTGGCCATAAAAACCCTGAAAGTTGGTTACACAGAAAAGCAAAGGAG GGACTTTTTATGCGAAGCAAGCATCATGGGGCAATTTGACCACCCAAATGTCGTCCATTTGGAAGGGGTT GTTACAAGAGGGAAGCCTGTCATGATTGTGATAGAGTTCATGGAGAATGGAGCCCTGGATGCATTTCTCA GGAAACACGATGGGCAGTTTACAGTCATTCAGTTGGTAGGAATGTTGAGAGGTATTGCCGCTGGGATGCG ATACTTGGCTGATATGGGATACGTTCACAGGGACCTTGCAGCGCGCAACATCCTTGTCAACAGCAATCTT GTTTGTAAAGTGTCAGATTTTGGCCTTTCCCGGGTTATAGAGGATGATCCCGAAGCTGTCTACACCACGA CTGGTGGAAAAATTCCAGTAAGGTGGACTGCACCGGAAGCCATTCAATACCGGAAGTTCACCTCAGCCAG CGATGTGTGGAGCTATGGGATTGTCATGTGGGAAGTGATGTCTTATGGAGAAAGACCTTACTGGGACATG TCAAATCAAGATGTCATTAAAGCGATAGAAGAAGGTTATCGTTTGCCGGCGCCCATGGATTGCCCAGCTG GTCTTCACCAGCTAATGCTGGATTGTTGGCAGAAAGATCGGGCGGAAAGGCCAAAGTTTGAGCAGATAGT CGGAATTCTAGACAAAATGATTCGAAACCCAAGTAGTCTGAAAACACCCCTGGGAACTTGTAGTAGACCC TTAAGCCCTCTTCTGGACCAGAGCACTCCTGACTTCACTGCCTTCTGTTCAGTTGGAGAATGGTTGCAAG CTATTAAAATGGAAAGGTATAAGGACAACTTCACAGCAGCGGGTTACAACTCACTCGAGTCAGTGGCCAG GATGACTATCGATGATGTGATGAGTTTAGGGATCACACTGGTTGGCCATCAAAAGAAGATCATGAGCAGC ATCCAGACTATGCGGGCACAAATGTTGCATTTACACGGAACAGGCATCCAAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226270 representing NM_010141
Red=Cloning site Green=Tags(s) MVVQTRFPSWIILCYIWLLGFAHTGEAQAAKEVLLLDSKAQQTELEWISSPPSGWEEISGLDENYTPIRT YQVCQVMEPNQNNWLRTNWISKGNAQRIFVELKFTLRDCNSLPGVLGTCKETFNLYYYETDYDTGRNIRE NLYVKIDTIAADESFTQGDLGERKMKLNTEVREIGPLSKKGFYLAFQDVGACIALVSVKVYYKKCWSIVE NLAVFPDTVTGSEFSSLVEVRGTCVSSAEEEAENSPRMHCSAEGEWLVPIGKCICKAGYQQKGDTCEPCG RRFYKSSSQDLQCSRCPTHSFSDREGSSRCECEDGYYRAPSDPPYVACTRPPSAPQNLIFNINQTTVSLE WSPPADNGGRNDVTYRILCKRCSWEQGECVPCGSNIGYMPQQTGLEDNYVTVMDLLAHANYTFEVEAVNG VSDLSRSQRLFAAVSITTGQAAPSQVSGVMKERVLQRSVQLSWQEPEHPNGVITEYEIKYYEKDQRERTY STLKTKSTSASINNLKPGTVYVFQIRAVTAAGYGNYSPRLDVATLEEASGKMFEATAVSSEQNPVIIIAV VAVAGTIILVFMVFGFIIGRRHCGYSKADQEGDEELYFHFKFPGTKTYIDPETYEDPNRAVHQFAKELDA SCIKIERVIGAGEFGEVCSGRLKLPGKRDVAVAIKTLKVGYTEKQRRDFLCEASIMGQFDHPNVVHLEGV VTRGKPVMIVIEFMENGALDAFLRKHDGQFTVIQLVGMLRGIAAGMRYLADMGYVHRDLAARNILVNSNL VCKVSDFGLSRVIEDDPEAVYTTTGGKIPVRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDM SNQDVIKAIEEGYRLPAPMDCPAGLHQLMLDCWQKDRAERPKFEQIVGILDKMIRNPSSLKTPLGTCSRP LSPLLDQSTPDFTAFCSVGEWLQAIKMERYKDNFTAAGYNSLESVARMTIDDVMSLGITLVGHQKKIMSS IQTMRAQMLHLHGTGIQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010141 |
| ORF Size | 2994 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010141.4, NP_034271.3 |
| RefSeq Size | 6732 bp |
| RefSeq ORF | 2997 bp |
| Locus ID | 13841 |
| UniProt ID | Q61772 |
| MW | 112.3 kDa |
| Gene Summary | Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 is a cognate/functional ligand for EPHA7 and their interaction regulates brain development modulating cell-cell adhesion and repulsion. Has a repellent activity on axons and is for instance involved in the guidance of corticothalamic axons and in the proper topographic mapping of retinal axons to the colliculus. May also regulate brain development through a caspase(CASP3)-dependent proapoptotic activity. Forward signaling may result in activation of components of the ERK signaling pathway including MAP2K1, MAP2K2, MAPK1 AND MAPK3 which are phosphorylated upon activation of EPHA7. Isoform 4 which lacks the kinase domain may regulate isoform 1 adhesive properties.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223037 | Epha7 (untagged) - Mouse Eph receptor A7 (Epha7), transcript variant 1, (10ug) |
CNY 12,920.00 |
|
| MG226270 | Epha7 (tGFP-tagged) - Mouse Eph receptor A7 (Epha7) transcript variant 1, (10ug) |
CNY 9,600.00 |
|
| MR226270L3 | Lenti ORF clone of Epha7 (Myc-DDK-tagged) - Mouse Eph receptor A7 (Epha7), transcript variant 1 |
CNY 10,640.00 |
|
| MR226270L4 | Lenti ORF clone of Epha7 (mGFP-tagged) - Mouse Eph receptor A7 (Epha7), transcript variant 1 |
CNY 10,640.00 |
