Clcn4 (NM_001302386) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	clC-4; Clc4-2; Clcn4-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230885 representing NM_001302386 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTACTCATTGGGCTGCTGGCAGGTACCTTGGCTGGAGTCATCGATCTCGCTGTGGATTGGATGACGG ACCTCAAGGAGGGGGTCTGTCTGTCCGCATTCTGGTACAGCCATGAACAGTGCTGTTGGACCTCCAACGA GACCACTTTTGAGGACAGGGACAAGTGTCCCCTGTGGCAGAAGTGGTCAGAGCTTCTTCTGAGCCAGTCA GAGGGCGCCAGCGCTTACATTCTGAATTACTTAATGTACATTCTATGGGCGTTGCTGTTTGCATTTCTGG CTGTCTCCCTGGTACGTGTGTTCGCACCGTATGCCTGTGGCTCTGGCATACCCGAGATAAAGACTATTTT GAGTGGCTTTATCATCAGGGGATACTTGGGGAAATGGACTCTTCTAATCAAGACTGTCACCCTCGTGCTC GTCGTATCCTCTGGCCTGAGCCTTGGCAAAGAGGGCCCACTGGTGCATGTGGCATGTTGCTGTGGCAACT TCTTCAGCAGCCTTTTCTCCAAGTATAGCAAGAATGAAGGCAAGAGGCGTGAGGTGCTTTCAGCTGCAGC TGCTGCTGGTGTCTCTGTGGCCTTTGGTGCTCCGATAGGAGGTGTGCTCTTCAGTCTAGAGGAGGTCAGT TACTACTTTCCCTTGAAAACCTTGTGGAGGTCATTCTTTGCAGCCCTGGTGGCTGCCTTCACACTGCGCT CCATCAACCCCTTTGGAAATAGCCGCCTGGTTCTCTTTTATGTGGAGTATCATACACCCTGGTACATGGC TGAACTCTTCCCTTTCATCCTGCTTGGAGTCTTTGGGGGTTTATGGGGAACCCTCTTCACACGCTGCAAC ATTGCTTGGTGCAGGAGGCGTAAGACCACCAGGCTGGGCAGGTACCCAGTGTTGGAGGTTATTGCGGTGA CAGCCGTCACCGCCATCGTGGCCTACCCCAATCCCTACACTCGCCAGAGCACCAGTGAGCTCATCTCTGA GCTCTTCAACGATTGTGGGGCTCTCGAGTCTTCTCAGCTCTGTGACTACATCAACGACCCCAACATGACT CGGCCTGTGGATGACATTCCGGACCGGCCGGCTGGGGTTGGAGTTTACACAGCCATGTGGCAGCTGGCCT TGGCACTGATCTTCAAAATAGTCATTACTATATTTACCTTTGGCATGAAGATTCCCTCAGGTCTCTTCAT CCCCAGTATGGCTGTCGGAGCCATGGCAGGCCGGATGGTGGGAATCGGTGTGGAGCAGCTGGCCTACCAT CACCATGACTGGATCATCTTCAGGAACTGGTGCAGGCCTGGAGCGGACTGTGTCACACCAGGGCTTTATG CGATGGTGGGAGCTGCAGCCTGTCTAGGTGGGGTGACTAGGATGACAGTGTCTCTAGTGGTCATTATGTT TGAACTGACTGGAGGTCTGGAGTATATTGTACCCCTGATGGCAGCTGCTGTCACCAGCAAGTGGGTGGCT GATGCCTTTGGGAAAGAAGGGATTTATGAAGCCCACATCCATCTGAATGGGTACCCATTTCTTGATGTGA AGGATGAGTTCACCCACCGTACGCTGGCCACTGATGTGATGCGGCCCCGGAGGGGAGAACCGCCATTATC GGTACTAACCCAGGACAGCATGACTGTGGAGGACGTGGAGACTCTCATCAAGGAGACAGACTACAACGGC TTTCCTGTGCTCGTCTCCAGAGACTCGGAGCGTCTCATCGGGTTTGCCCAGAGGCGGGAGCTAATCTTGG CTATAAAAAATGCCAGGCAGAGGCAAGAGGGCATTGTGAGCAATTCCATCATGTACTTCACAGAGGAGCC TCCTGAGCTGCCTGCCAACAGCCCACATCCACTGAAGCTGAGGCGCATTTTGAACCTGAGCCCTTTCACG GTCACAGATCACACCCCCATGGAGACGGTGGTGGACATTTTCCGGAAACTGGGGCTCCGACAATGCCTGG TGACACGGAGTGGGAGACTTCTTGGGATCATCACAAAAAAGGATGTTCTGAGACACATGGCCCAGATGGC AAACCAGGACCCTGAATCCATCATGTTTAAT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGA TTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230885 representing NM_001302386 Red=Cloning site Green=Tags(s) MLLIGLLAGTLAGVIDLAVDWMTDLKEGVCLSAFWYSHEQCCWTSNETTFEDRDKCPLWQKWSELLLSQS EGASAYILNYLMYILWALLFAFLAVSLVRVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLLIKTVTLVL VVSSGLSLGKEGPLVHVACCCGNFFSSLFSKYSKNEGKRREVLSAAAAAGVSVAFGAPIGGVLFSLEEVS YYFPLKTLWRSFFAALVAAFTLRSINPFGNSRLVLFYVEYHTPWYMAELFPFILLGVFGGLWGTLFTRCN IAWCRRRKTTRLGRYPVLEVIAVTAVTAIVAYPNPYTRQSTSELISELFNDCGALESSQLCDYINDPNMT RPVDDIPDRPAGVGVYTAMWQLALALIFKIVITIFTFGMKIPSGLFIPSMAVGAMAGRMVGIGVEQLAYH HHDWIIFRNWCRPGADCVTPGLYAMVGAAACLGGVTRMTVSLVVIMFELTGGLEYIVPLMAAAVTSKWVA DAFGKEGIYEAHIHLNGYPFLDVKDEFTHRTLATDVMRPRRGEPPLSVLTQDSMTVEDVETLIKETDYNG FPVLVSRDSERLIGFAQRRELILAIKNARQRQEGIVSNSIMYFTEEPPELPANSPHPLKLRRILNLSPFT VTDHTPMETVVDIFRKLGLRQCLVTRSGRLLGIITKKDVLRHMAQMANQDPESIMFN TRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-NotI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001302386
ORF Size	2061 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001302386.1, NP_001289315.1
RefSeq Size	4169 bp
RefSeq ORF	2064 bp
Locus ID	12727
MW	76.9 kDa
Gene Summary	Proton-coupled chloride transporter. Functions as antiport system and exchanges chloride ions against protons (By similarity).[UniProtKB/Swiss-Prot Function]

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